Varied TCM constitutions in the patient of acute cerebral infarction were classifyed by using HLA - DQAl allehc gene to analyze hereditary susceptibility of constitution types and relation among constitution, syndrome and treatment. PCR-SSP technique was used for classification of Yin - deficiency Yang - deficiency, Qi - deficiency, phlegm - dampness and blood stasis constitution in 103 cases of acute cerebral infarction and HLA - DQAl allelic gene was used for gent classification in 99 cases of nomal constituion. Results indicated that HLA -DQA * 0501 gene type in Yin -deficiency constitution was significant higher than that of normal constitution CP

Objective:Comparing the influence of colon cancer recurrence of laparoscopic and open operation to evaluate the safety and validity of operation.Methods:The cohort study includes 62 cases of laparoscopic and 83 cases of open colon cancer radical surgery from January 2010 to October 2012.The research aimed at comparing the effects of laparoscopic and open surgery for colon cancer recurrence by analyzing postoperative follow-up ending and GCC-mRNA & CEA.Results:There have no obvious differences in age,sex,BMI,tumor size,stage,grade,pathological types and resection range between laparoscopy and open group(P＞0.05).Postoperative 3-years recurrence of two groups have no difference (P＞0.05),but 1-year recurrence rate of laparoscopic was obviously lower than open operation (3.2％ vs 14.5％,P＜0.05).There have no obvious differences of postoperative overall survival rate and mortality(P＞0.05),but 1-year disease-free survival rate of laparoscopic group was obviously higher than laparotomy group (93.5％ vs 81.3％,P＜0.05).The postoperative GCC-mRNA and CEA positive rate of laparoscopic was significantly lower than laparotomy group.Univariable and Multivariable analysis reveals that laparoscopic colorectal cancer radical can obviously reduce the cancer recurrence within 1 year (P＜0.05).Addition,stage,aspirin and other amino acid drugs are independent risk factors of postoperative recurrence (P＜0.05).Conclusion:Laparoscopic colon cancer radical surgery is a safe and effective operation and can reduce short-term recurrence of colorectal cancer compared to open surgery.

Objective To explore the relationship between the expression of VEGF and TGF-β1 in naso-pharyngeal carcinoma and the clinical characteristics and prognosis of nasopharyngeal carcinoma patients. Meth-ods The expression of VEGF and TGF-β1 in the local middle-late nasopharyngeal carcinoma tissue of 96 patients was detected using immunohistochemistry.The correlation and the prognosis analysis were analyzed in combination with their clinical characteristics and prognosis. Results Expression rate of VEGF was 66.7% and that of TGF-β 1 53.1%.The expression of VEGF and TGF-β1 were positively correlated in the advanced nasopharyngeal carcino-ma.Both VEGF and TGF-β1 had significant correlation with lymphatic metastasis.The effects of VEGF and TGF-β1 on MFS were analyzed by Cox single factor and the results showed that VEGF positive was an adverse prognostic factor(P<0.05).The effects of age,sex,lymphatic metastasis,local invasion,VEGF and TGF-β1 on MFS were analyzed with Cox multifactor analysis and the results showed that VEGF positive was an adverse prognostic factor (P < 0.01). Conclusion The detection of VEGF and TGF-β1 protein in nasopharyngeal carcinoma may help to predict the metastasis of nasopharyngeal carcinoma.