Objective:To explore any effect of 8 weeks of high-intensity interval training (HIIT) on glycemia and pancreatic β-cell function among persons with type 2 diabetes to provide data for optimizing their exercise protocol.Methods:Sixty patients with type 2 diabetes and without a habit of regular exercise were randomly divided into an exercise group ( n=30) and a control group ( n=30). Both groups maintained their daily living habits, except that the exercise group practiced HIIT on a power vehicle ergometer 3 times a week for 8 weeks. Before and after the intervention, the 2-hour oral glucose tolerance test (OGTT) was conducted to evaluate glycemia and pancreatic β-cell function. Body composition was also detected using dual-energy X-ray absorptiometry. Results:After the intervention a significant decrease was observed in the fasting blood glucose, mean blood glucose, glycosylated hemoglobin, blood glucose levels at the end of a 2h OGTT, blood glucose area under the curve and homeostatic model assessment of insulin resistance, as well as waist circumference and abdominal fat content of the exercise group. And there was a significant increase in the homeostatic model assessment of pancreatic β-cell function and disposition index among the exercise group. In the control group no significant differences were observed.Conclusion:Eight weeks of HIIT can improve glycemia and pancreatic β-cell function and reduce abdominal fat among persons with type 2 diabetes. It can be used as an effective rehabilitation protocol.

[Abstract] Objective: To explore the mechanism of miR-145-5p on malignant biological behaviors, such as pro-liferation, invasion, migration and epithelial-mesenchymal transition (EMT), of esophageal squamous cell carcinoma (ESCC) TE-10 cells. Methods: The expression of miR-145-5p in ESCC cell lines and normal cells was detected by PCR. Dual luciferase reporter gene assay was used to detect the targeted regulation between miR-145-5p and insulin-like growth factor 1 receptor (IGF1R). The expres-sions of IGF1R protein and EMT related proteins were detected by Western blotting. Transwell assay and CCK-8 assay were carried out to detect the effects of miR-145-5p/IGF1R axis on the proliferation, migration andinvasionofTE-10 cells. Results: miR-145-5p was down-regulated in ESCC cell lines with the lowest expression in TE-10 cells (P<0.01orP<0.05).Over-expression of miR-145-5p significantly inhibited proliferation, invasion, migration and EMT of TE-10 cells (P<0.01 or P<0.05). Dual luciferase reporter gene assay con-firmed that miR-145-5p targetedly down-regulated IGF1R expression (P<0.01). The restora-tion experiments further confirmed that simultaneous over-expression of miR-145-5p and IGF1R significantly attenuated the promotion effect of IGF1R on proliferation, invasion, migration and EMT of TE-10 cells (P<0.01 or P<0.05). Conclusions: Over-expression of miR-145-5p inhibits proliferation, invasion, migration and EMT of ESCC TE-10 cells by down-regulating IGF1R.

With the broad application of high-resolution computed tomography (CT) and high rates of early lung cancer screening, the number of patients diagnosed with synchronous multiple primary lung cancer (sMPLC) has been increasing. It becomes of great prominence to distinct sMPLC from intrapulmonary metastases in clinical practice. An increasing number of studies have developed high-throughput sequencing based genetic approaches to specify the molecular characteristics of sMPLC, which contributes to a better understanding of its tumorigenesis. The genetic profile of sMPLC also benefits its diagnosis, which mainly relies on its clinicopathological criteria. Here, we summarize the progresses on the diagnostic criteria for sMPLC, and also molecular features of sMPLC from the perspective of clonality analysis.