A method for partially purifying the native human gamma interferon (HuIFN-?) was presented. The rude HuIFN-?was concentrated by PEG 4 000, precipitated by (NH_4)_2SO_4 and purified by CM-Sephades C-50(more than 100 times). The titre of HuIFN-?was 1.3X10~5u/ml and its specific activity was 1. 0 X 10~5u/mg protien. Quality control tests were performed and 10 volunteers received intramuscularly the purified HuIFN-?without significant changes in body temperature, pulse rate and blood pressure. It is considered to be suitable for clinical trial.

The biochemical characteristics of protein factor(PF)purified from 3～5 month fetal liver cellsincluding pH,content of protein molecular weight,sequences of amino acid,?-fetal protein,tracelements and HBsAg,were determined.The PF was tested on the model of duck hepatic B virus anused to treated the rat acute hepatic failure,and its role in inhibiting the PLC cells to secrete HBsAwas tested.The result showed that the PF could meet with the clinical standdar for musculainjection.The PF can suppress the synthsis of hepatitis B virus,increasing the survival rate of animawith hepatic failure,improving liver function,stimulating the synthesis of DNA in liver and speed uthe recovery of liver tissue.