By using immunocolloidal gold-silver technique and ABC technique, an antihuman von Willebrand factor (vWF) monoclonal antibody, SZ-29, was applied to study the distribution of vWF in vessels and platelets. The results demonstrated that vWF distributed in the endothelium, basal membrane, subendothelium and media near internal elastic membrane of abdominal aorta, umbilical artery and umbilical vein. And the densest vWF-specific staining was found in the endothelium and its basal membrane. At the microscopical level, we found that vWF-specific staining was granule--like and scattered in most platelets, but about 3-5% of the platelets were found entirely silver-stained. At the ultrastructural level, we found that vWF was stored in ?-granules and there was little platelet membrane-bound vWF in resting platelets. However, in stimulated platelets, plenty of membrane-bound vWF was found on the platelet surface and open canalicular system membrane besides the vWF-specific staining in ?-granules. And vWF-specific staining might be found in platelet pseudopodia, especially at their tips,

Objective To study and contruct competency characteristic evaluation models of nursing supervisors in secondly hospitals.Methods Using the matron semi-structured interviews,literature review,questionnaire survey and expert questionnaires to discuss the competency of nursing supervisors in secodary hospitals.Results We found that the competency model of nursing supervisors in secondary hospitals could better reflect the competency of nursing supervisors.The model had 16 competencies which included knewleage skills.management and individual characters.Conclusions Through the study of the model of competency nursing supervisors in secondary hospitals,we form a set of standard for nursing supervisor selection based on their competency.The standard can help to select the most suited nursing supervisors who have great potentials.

Neck dissection for oral squamous-cell carcinoma (OSCC) is a clinically controversial issue and has therefore been the subject of abundant research. However, no one has performed a bibliometric study on this topic to date. The aim of this study was to assess the development of research on neck dissection for OSCC in terms of the historical evolution, current hotspots and future directions, particularly including research trends and frontiers from 2010 to 2019. Literature records related to research on neck dissection for OSCC were retrieved from the Web of Science Core Collection (WoSCC). CiteSpace was used as a tool to perform a bibliometric analysis of this topic. The survey included 2 096 papers. "Otorhinolaryngology" was the most popular research area. The most active institutions and countries were Memorial Sloan Kettering Cancer Center and the USA, respectively. Shah J.P. was the most cited author. Among the six identified "core journals", Head & Neck ranked first. The top three trending keywords were 'invasion', 'upper aerodigestive' and 'negative neck'. 'D'Cruz AK (2015)' was the most cited and the strongest burst reference in the last decade. The study evaluated the effect on survival of elective versus therapeutic neck dissection in patients with lateralized early-stage OSCC. The depth of invasion and the management of N0 OSCC were research frontiers in this field. The present study provides a comprehensive bibliometric analysis of research on neck dissection for OSCC, which will assist investigators in exploring potential research directions.
Bibliometrics ; Carcinoma, Squamous Cell/surgery* ; Head and Neck Neoplasms ; Humans ; Mouth Neoplasms/surgery* ; Neck Dissection