OBJECTIVE:To study the inhibitory effects of minocycline (MC) on TNF-α induced monocyte-endothelial adhe-sion and the relative mechanism. METHODS:Primary human umbilical vein endothelial cells (HUVECs) were isolated from hu-man umbilical veins with enzyme digestion. HUVECs were divided into blank control group,model group,MC low-dose,medi-um-dose and high-dose groups(1,10,100 μmol/L). After treated for 2 h,10 ng/ml TNF-α was employed to stimulate monocytes THP-1 adhesion with HUVECs except for blank control group,in order to induce monocyte-endothelial adhesion model. The num-ber of adherent cell was observed by fluorescence microscope,and fluorescence intensity was detected by microplate reader. Flow cytometry was adopted to detect the expression of intercellular adhesion molecule (ICAM)-1. The expressions of NF-κB p65 pro-tein in cell nucleus and cytoplasm were detected by Western blot. RESULTS:Compared with blank control group,the number and fluorescence intensity of adherent cell,the expression of ICAM-1 and the protein expression of NF-κB p65 in nucleus were all in-creased in model group,while the protein expression of NF-κB p65 in cytoplasm was weakened,with statistical significance(P<0.01). Compared with model group,the number and fluorescence intensity of adherent cell,the expression of ICAM-1 were all de-creased in MC low-dose,medium-dose and high-dose groups;the protein expression of NF-κB p65 in nucleus was weakened in MC medium-dose and high-dose groups,while the protein expression of NF-κB p65 in cytoplasm was heightened,with statistical significance (P<0.01 or P<0.05). CONCLUSIONS:MC can inhibit TNF-α induced monocyte-endothelial adhesion by a likely mechanism of reducing the expression of ICAM-1 in HUVECs and inhibiting the expression of NF-κB p65 protein.

Objective:To establish a method for simultaneous determination of 7 elemental impurities(V,Co,Ni,As,Cd,Hg Pb)in amlodipine besylate tablets based on inductively coupled plasma mass spectrometry(ICP-MS).Methods:After the samples were treated by microwave digestion,the solution was analyzed by ICP-MS.Ge,In,Bi were selected as the internal standards.The established method was validated.The contents of 7 elemental impurities in amlodipine besylate tablets from 54 enterprises were determined by this method.Results:The content of(V,Co,Ni,As,Cd,Hg,Pb had good linear relationship in the ranges of 1-100,1-100,1-100,1-100,1-100,0.5-4,1-100 ng·mL-1,respectively.The correlation coefficients(r)all above 0.999 4.The detec-tion limits and quantification limits were in the range of 0.000 4-0.018 4 ng·mL-1 and 0.001 4-0.061 2 ng·mL-1.The RSD of precision was less then 1.8％.The RSD of repeatability was less then 6.1％.The average re-coveries(n=9)were between 85.4％-106.5％,while their RSD was less then 4.7％.The content of 7 elemental impurities in 54 sample batches were in accordance with the limit value.Conclusion:The method is simple,rapid,accurate,reliable and highly sensitive,and can be used for the quality control of elemental impurities in amlodipine besylate tablets.