OBJECTIVETo evaluate the sensitization effect of polysorbate 80 from different factories on Beagle dogs.

METHODBeagles dogs were randomly divided into 5 groups, 3 in each group, received respectively the intravenous infusion of polysorbate 80 made by four factories in the concentration of 0.5%, with the constant infusing speed of 5 mL x min(-1) and volume of 10 mL x kg(-1). Changes were observed before infusion and in the 24 h after infusion, time of symptom appearance and disappearance was recorded, and the grade of response was determined. Moreover, blood pressure and heart rates were tested by the machine of Bp-98E, blood samples of animals were collected before infusion and at 10 min after ending infusion for measuring histamine content in plasma using ELISA. Then the sensitization effect was comprehensively estimated by combined consideration of the responding grade and histamine level.

RESULTTypical symptoms of anaphylactoid reactions were found in sample 3 group, atypical symptoms were found in other polysorbate 80 groups. Different degrees of heart rate speeding and blood pressure downing were found in polysorbate 80 groups. No over 1-fold increase of plasma histamine level was found in all groups. The atypical anaphylactoid reactions and blood pressure of sample 2 group was lighter than other polysorbate 80 groups. Estimation showed that the sample 3 induced the suspicious anaphylactoid reactions, other test solutions did not induce the typical anaphylactoid reactions on Beagle dogs.

CONCLUSIONFor allergies and other special populations, there is still a certain risk to applicate polysorbate 80 in the concentration of 0.5%. Production process of polysorbate 80 have a certain influence on allergenicity, refined polysorbate 80 increase the security, but further reasearchs are needed to confirmed.

Anaphylaxis ; blood ; chemically induced ; physiopathology ; Animals ; Blood Pressure ; Dogs ; Dose-Response Relationship, Drug ; Drug Hypersensitivity ; blood ; physiopathology ; Histamine ; blood ; Male ; Polysorbates ; adverse effects

The development of living cell drugs and their successful application in clinical treatments require full clarification of the fate of cells after transplantation, which is critical to the safety and efficacy of living cell drugs.In order to solve this problem, cell imaging technology has come into our sight, and the use of visualization technology for non-invasive tracing of living cell drugs could reveal the distribution, homing and activity of living cell drugs in the body, which helps to determine the best number of transplanted cells, optimize the administration scheme, improve the transplantation efficiency, enhance the targeting of transplanted cells, and reduce the potential off-target accumulation risk.This paper summarizes the research advances of non-invasive visual tracing in vivo for living cell drugs from the perspectives of radionuclide imaging, magnetic resonance imaging, magnetic particle imaging, computed tomography imaging, fluorescence imaging and multimodal imaging.The aim is to obtain the biological behavior of living cell drugs in vivo with the application of appropriate contrast agent and tracing technology, and provide a more reasonable scientific basis for the research and development of living cell drugs and their transplantation therapy.

AIM: To investigate the regulatory effects of silybin on hepatic lipid metabolism in mice with non -alcoholic steatohepatitis (NASH) induced by high - fat and high-cholesterol (HFHD) diet. METHODS: Mice were fed a HFHD diet to construct a NASH model, and serum levels of triacylglycerol (TAG), total cholesterol (T-CHO), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were measured using biochemical kits. H&E staining and oil red O staining were used to detect histopathological changes in the liver. Lipidomics was used to detect the alterations of hepatic lipid metabolism in NASH mice. RESULTS: Silybin significantly inhibited the increase of body weight, liver weight and abdominal fat, decreased serum T-CHO, TAG and LDL-C levels, improved hepatic lipid droplet accumulation and ballooning degeneration, and back-regulated hepatic palmitoleic acid (C16: 1) and polyunsaturated long-chain fatty acids (PUFAs) in NASH mice. CONCLUSION: Silybin possibly reduced hepatic lipid accumulation and lipotoxicity by modulating abnormal hepatic lipid metabolism in mice induced by HFHC diet.