1.Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat.
Hend Shafik BASIOUNY ; Nagla Mohamed SALAMA ; Zeinab Mohamed EL MAADAWI ; Eman Abas FARAG
International Journal of Stem Cells 2013;6(1):12-25
BACKGROUND AND OBJECTIVES: Mesenchymal stem cells have delivered new approaches to the management of wound healing in severe skin injuries. This work was planned to evaluate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on healing of induced full thickness skin wounds in albino rats using topical & systemic injections. METHODS AND RESULTS: Forty adult male albino rats were classified into 2 groups after induction of full thickness skin wound; untreated group and stem cell-treated group. The latter was further subdivided into topically and systemically treated ones. BMSCs were isolated & labeled by PKH26 before injection. Healing of wounds was evaluated grossly. Skin biopsies were obtained one & three weeks after wound induction. Sections were stained with Hematoxylin & Eosin, Masson's trichrome and immunohistochemichal stain for vascular endothelial growth factor (VEGF). Epidermal thicknesses and mean area percent of both collagen fibers & VEGF immunopositive cells were measured using image analyzer & results were subjected to statistical analysis. PKH26 fluorescent-labeled cells were found in the regenerated epidermis, hair follicles and dermis in BMSCs-treated groups. By the end of the third week, the wounds of BMSCs-treated groups showed full regeneration of epidermis, re-organization of collagen and decrease in VEGF immunopositive cells. Delayed wound healing was seen in 20% of systemically treated rats. Significant increase in the mean area percent of collagen fibers was detected in topically treated group. CONCLUSIONS: Both methods of BMSCs injection were effective in healing of full thickness skin wound but topical method was more effective.
Adult
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Animals
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Biopsy
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Bone Marrow
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Collagen
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Dermis
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Eosine Yellowish-(YS)
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Epidermis
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Hair Follicle
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Hematoxylin
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Humans
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Male
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Mesenchymal Stromal Cells
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Organic Chemicals
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Rats
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Regeneration
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Skin
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Vascular Endothelial Growth Factor A
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Wound Healing