Zonulolytic effects of alpha-chymotrypsin and trypsin were examined on rabbits' eyes using an apparatus which enables one to press the enucleated eyeballs. The time interval between the applications of enzymes and the protrusion of the lens was measured. The results showed that the trypsin was 4 to 5 times more effective than alpha-chymotrypsin.
Trypsin*

The effects of antihypertensive drugs on the choroidal blood flow in rabbits were studied by an apparatus based on the principle of internal calorimetry of Grayson. Thermistors, as the sensing elements, were fastened on the scleral surface of the eye, and determinations were performed up to 60 minutes after intravenous administrations of drugs. The drugs studied were: ganglion blocking agents (pentholinium tartarate, 4 mg; hexamethonium bromide, 1 mg; and mecamylamine chloride, 0.15mg), alpha-methyldopa, 4 mg; guanethidine, 0.5 mg; reserpine, 0.2 mg; hydralazine, 5 mg; and diuretics (dichlorothiazide, 2.5 mg; frusemide, 2.5 mg). Except the diuretics, all the drugs employed produced considerable increase in the choroidal blood flow. The relationships between blood pressure, intraocular pressure and the choroidal blood flow were discussed and the clinical applications were suggested.
Administration, Intravenous ; Antihypertensive Agents* ; Blood Pressure ; Calorimetry ; Choroid* ; Dental Calculus ; Diuretics ; Furosemide ; Ganglion Cysts ; Guanethidine ; Hexamethonium ; Hydralazine ; Intraocular Pressure ; Mecamylamine ; Methyldopa ; Rabbits* ; Reserpine

The posterior surface of the rabbit's cornea was scraped by a spatula or incised by a cystotome, followed by injection of alpha-chymotrypsin or trypsin into the anterior chamber. The concentration of enzymes used were 5mg/cc and O.5mg/cc of alpha-chymotrypsin and 5.000 Anson unit/cc and 500unit/ cc of trypsin. Three minutes after the introduction of enzymes into the anterior chamber, irrigation of the anterior chamber with normal saline was performed. The cornea was examined macroscopically and biomicroscopica11y for about 3 weeks. Histological examination was done on the 3rd and the 11th post-operative days. The cornea was affected more intensively and extensively when the stroma was injured than when only the endothelium was injured. Alpha-chymotrypsin was more toxic to the cornea than trypsin and led sometimes to a corneal perforation.
Anterior Chamber ; Cornea* ; Corneal Perforation ; Endothelium ; Trypsin*