Objective To explore the effects of cannabinoid receptor agonist WIN55,212-2 preconditioning on spinal cord ischemia reperfusion injury in rats.Methods A total of 32 male Sprague-Dawley rats was randomly divided into four groups (n =8):sham group,control group,dimethyl sulfoxide(DMSO) group which was given intraperitoneally DMSO 0.3 ml 30 min before ischemia reperfusion,and WIN group which was given intraperitoneally WIN55,212-2 1 mg/kg 30 min before ischemia reperfusion.Each rat was neurologically assessed at 24 h and 48 h after reperfusion by Tarlov scale,and the number of normal motor neurons at anterior horn of the spinal cord was recorded.Res uits The Tarlov scale of WIN group was significantly higher than that control and DMSO groups (P ＜ 0.05).There were more normal motor neurons at anterior horn of the spinal cord in WIN group than those in control and DMSO groups (P ＜ 0.05).Conclusions Cannabinoid receptor agonist WIN55,212-2 preconditioning might attenuate spinal cord ischemia reperfusion injury.

Objective To explore the features of gallbladder adenomyoma in sonogram, and improve the abilities of diagnosis and differential diagnosis. Method Sonograms of 8 patients with proven gallbladder adenomyoma were retrospectively analyzed and correlated with pathologic findings. Results Eight patients had sonographic evidence of localized intramural nodular,which were round-like and hypoechoic,with anechoic space and tiny bright echos, confined almost exclusively to the fundus of gallbladder. Five patients were also evident with associated gallstones and chronic cholecytitis. Conclusion Sonograms of gallbladder adenomyoma have marked features, which can be differentially diagnosed from other projective lesions of gallbladder.

OBJECTIVE:To study the effect of different administration time of ibuprofen on the changing characteristics of circadian rhythm about body temperature and plasma concentration in rats.METHODS:60 rats were given single oral administration of ibuprofen at the dose of 50 mg per kilogram at different time(2∶00,6∶00,10∶00,14∶00,18∶00,22∶00) according to a randomized block design with another 10 rats serving as blank control.The effects of ibuprofen on circadian rhythm variations of body temperature and plasma concentration in rats were observed and analyzed using chronogram and cosine method.RESULTS:The body temperature of rats prior to and after administration both showed a significant circadian rhythm,with mean declined only,yet acrophase and amplitude showed no significant differences.The best cosinor curve equation were Yt i=38.7+0.52 Cos(15??ti—86.80?) and Yt i=37.8+0.55 Cos(15??ti—117?) respectively.The plasma concentration also showed a significant circadian change in rats at the different time points of administration.The plasma concentration in light phase was higher than that in dark phase,and the best cosinor curve equation was Yt i=41.95+5.30 Cos(15??ti—272.83?).CONCLUSION:The differences of circadian rhythm were both shown about body temperature and plasma concentration in rats when ibuprofen was orally administered at the different time points.

Animals ; Female ; Hippocampus ; physiology ; Learning ; Long-Term Potentiation ; Male ; Memory ; Mice ; Mice, Inbred Strains ; Pregnancy ; Prenatal Exposure Delayed Effects ; psychology ; Tobacco Smoke Pollution

Autoimmune Diseases ; chemically induced ; Female ; Humans ; Interferons ; adverse effects ; Purpura, Thrombocytopenic, Idiopathic ; chemically induced ; Young Adult

Animals ; Hippocampus ; metabolism ; Long-Term Potentiation ; Male ; Neurogranin ; biosynthesis ; Rats ; Rats, Wistar ; Sleep Deprivation ; metabolism

Objective To investigate the effects of the combination of tripterygium wilfordii Hook F (TwHF) and irbesartan on urinary podocyte in diabetic kidney disease (DKD) patients,and to discuss the mechanism of protective effect of TwHF on DKD.Methods A total of 45 type 2 diabetic kidney disease patients were enrolled into this prospective study,and were randomly divided into 3 groups:TwHF treatment group (DT,n =15),irbesartan treatment group (DI,n =15),and TwHF combined with irbesartan treatment group (DTI,n =15).After 6 weeks washout,the 3 groups were given TwHF (1-2 mg · kg-1 ·d-1),irbesartan (150-300 mg/d),and TwHF (1-2 mg · kg-1 · d-1) combined with irbesartan (150-300 mg/d) for 12 weeks respectively.Fifteen healthy volunteers served as controls.Urinary podocytes were identified and quantitated by immunofluorescence staining of urinary sediments labeled by monoclonal antibody podocalyxin.In addition,we studied urinary connective tissue growth factor (CTGF),osteopontin (OPN) and transforming growth factor β1 (TGFβ1) concentrations in DKD patients by enzyme-linked immunosorbent assay.Results Urinary detached podocytes were obviously higher in the urine of DKD patients than in healthy controls (P ＜0.01).Podocyte detection rate was 86.6％ in the urine of DKD patients.The protein expressions of CTGF,OPN and TGFβ1 in patients with urinary podocyte were significantly increased than those without urinary podocyte (P ＜ 0.05 or 0.01).Correlation analysis showed that there was positive correlation between urinary protein excretion and urinary podocytes (r =0.79,P ＜ 0.01) and there were positive correlations between the number of urinary podocytes and urinary protein expressions of CTGF,OPN and TGFβ1 (r =0.56,0.41,0.44,respectively,all P values ＜ 0.01).Urinary albumin excretion and urinary podocytes were significantly decreased in all treatment groups (P ＜ 0.01),simultaneously,urinary concentrations of CTGF,OPN and TGFβ1 were reduced in all groups at week 12 after intervention of TwHF,irbesartan and TwHF combined with irbesartan (P ＜ 0.01),and these changes were more distinguished in combined treatment group (P ＜ 0.05).Conclusion Urinary podocyte in the urine may be suggested to be an early effective marker of disease activity in DKD.TwHF may be effective to prevent podocyte injury in DKD,which may be mediated,at least partly,by down-regulating the expression of CTGF,OPN and TGFβ1.There is a synergistic protective effect of TwHF combined with irbesartan on podocyte injury in DKD patients.

Objective To investigate clinical efficacy and safety of carboprost tromethamine injection combined with low B -Lynch suture for intractable postpartum hemorrhage placenta previa.Methods 125 cases of intractable postpartum hemorrhage placenta previa were divided into two groups according to treatment(n =62)with the observation group(n =63),the control group was administered oxytocin,and the line in the placental separation surface 8 interrupted suture;the observation group was used carboprost tromethamine injection combined with low B -Lynch suture.Clinical efficacy,blood loss,bleeding,postoperative bleeding 2h,24h after blood loss,transfusion rate and index sex hormone levels were compared before and after treatment.Results (1)After treatment,total effec-tive rate of the control group and observation group was 77.42%(48 /62)and 95.24%(60 /63)respectively.There was a statistically significant difference between the two groups(P <0.05);(2)Blood loss,bleeding time,2h after blood loss,blood loss and transfusion rate after 24h in the observation group were (1 608.37 ±43.01)mL,(8.89 ± 1.09)min,(101.18 ±8.73)mL,(221.17 ±20.58)mL and 4.76%,respectively,which were significantly lower than those of the control group [(1 992.28 ±55.62)mL,(12.83 ±1.77)min,(145.49 ±12.19)mL,(255.54 ± 23.30)mL and 16.13%](t =4.559,5.128,3.928,3.382,χ2 =5.559,all P <0.05);(3)before and after treatment indicator hormone(E2 ,FSH and LH)levels were not significantly different(P >0.05),and after treatment,the index level differences were not statistically significant(P >0.05);(4)the two groups during treatment,liver and kidney functions were normal,three cases of the control group and two cases of the observation group occured nausea and vomiting.Conclusion Carboprost tromethamine injection combined with low B -Lynch suture has significant effect, a small amount of bleeding,high security for intractable postpartum hemorrhage placenta previa,which should be promoted.

Objective To explore the effect of early drinking on keeping safety and improving comfort of mouth cavity in postoperative patients with transsphenoidal resection of pituitary adenoma. Methods A total of 70 postoperative patients with transsphenoidal resection of pituitary adenoma were divided into the experimental group and the control group with 35 cases each by random number table method. The control group were allowed to drink water after postoperative 6 hours, the experimental group were allowed to drink a small amount of water after 1 h when they recovered from anesthesia. The symptoms of heart rate, respiration, blood pressure, pulse oxygen saturation, nausea, vomiting, mis- aspiration, dry mouth,thirst, sore throat, oral pH value, oral cavity peculiar smell and the mood were observed 6 h after operation. Results After 6 hours, the heart rate, respiration, systolic blood pressure, and pulse oxygen saturation in the two groups were within normal range, which were (79.14±7.82) times per minute vs. (82.29±13.48) times per minute, (17.71± 1.10) times per minute vs. (18.31±1.92) times per minute, (121.66±10.61) mmHg vs.(121.55±12.86) mmHg (1 mmHg=0.133 kPa), (17.71±1.10) times per minute, (0.99±0.01) % vs. (0.99±0.01) %,there was no significant differences( t=-1.603-0.673, P > 0.05). The value of diastolic blood pressure in the control group were lower than that of the experimental group,(70.14±6.87) mmHg vs.( 74.34±7.29) mmHg, there was significant difference ( t=2.497, P < 0.05) .The incidence of mis- aspiration was zero in two groups. The rate of nausea, vomiting were 11.43%(4/35) and 5.71% (2/35) in the experimental group, which were significantly lower than 17.14%(6/35) and 11.43%(4/35) in the control group, there was no significant differences (χ2=0.467,0.182, P > 0.05). The rate of dry mouth,thirst in the experimental group were lower than those of the control group, 2.71±1.74 vs. 7.03± 1.98, 1.77±1.68 vs. 5.06±2.35, the difference was statistically significant (t=-9.685, -6.725, P < 0.01). T he value of sore throat in the experimental group was lower than that in the control group, 3.40±1.72 vs. 4.11± 2.23, there was no significant differences (t=-1.500, P > 0.05). There was difference in oral pH value(6.82±0.17 vs. 6.43±0.25, t=0.815, P < 0.01) between the experimental group and the control group. The rates of oral cavity peculiar smell and the mood in the experimental group were 8.57% (3/35) and 28.57% (10/35), which were better than those of the control group, 82.85% (29/35), 45.71%(16/35), the difference were significant (χ2=4.629, 10.516, P < 0.05). Conclusions Early drinking in postoperative patients with transsphenoidal resection of pituitary adenoma is secure and it can relieve uncomfortable symptoms, maintain normal oral pH value, improve comfort of mouth cavity and keep good mood of patients.

Objective:To study the efficacy of prostaglandin E1 in the patients with diabetic nephropathy and its inhibition on inflammatory factors. Methods:Totally 86 cases of diabetic nephropathy from June 2013 to June 2015 in our hospital were selected,and according to the random number,they were randomly divided into the observation group and the control group. The control group was treated with the conventional dietary restriction and therapy regimen including lowering blood pressure and blood sugar. The patients in the observation group were given prostaglandin E1 additionally. After the treatment,24h urine protein quantity,serum creatinine and blood urea nitrogen and the other basic indicators of renal function and the contents of sICAM-1 and hs-CRP were detected. Results:After the treatment,the contents of 24h urine protein,serum creatinine,blood urea nitrogen, sICAM-1 and hs-CRP in the two groups were notably decreased when compared with those before the treatment,and the decrease in the observation groups was more significant than that in the control group,and the difference was statistically significant(P <0. 05). During the course of treatment,no obvious adverse reactions appeared in the two groups. Conclusion:Prostaglandin E1 in the adjuvant treatment of diabetic nephropathy patients shows better therapeutic efficacy and inhibition on inflammatory factors with higher security,which should be promoted in clinics.