Objective To investigate the expression of COX-2 in multiple myeloma(MM)and the relationship between myeloma cells proliferation and apoptosis.To provide a new prognosis factor and therapeutic target.Methods COX-2 from the 22 newly diagnosed MM,14 relapsed MM and PCNA,HSP70 of the newly diagnosed patients were detected by immunohistochemistry method.Results All the newly diagnosed MM exhibited positive COX-2 immunoreactivity.50% had strong COX-2 and 50% showed weak COX-2.Relapsed MM exhibited strong COX-2.COX-2 was related with serum β2 microglobulin,marrow plasma cells,hemoglobin,PCNA,HSP70(P=0.019,0.003,0.048,0.006,0.034).Conclusion COX-2 was overexpressed in MM.Prognosis of patients with strong COX-2 is poorer than those with weak COX-2.COX-2 may promote the proliferation and inhibit the apoptosis of myeloma cells.

Acute Disease ; Adult ; Carbon Tetrachloride Poisoning ; diagnosis ; drug therapy ; Humans ; Male

Purpose To explore the ultrasonic characteristics of idiopathic retroperitoneal fibrosis (IRPF) and assess the diagnostic value of ultrasound. Materials and Methods Ultrasonic images of 11 cases of IRPF were retrospectively analyzed.Results The hypoechogenic masses encasing the abdominal aorta were detected in all cases, among which the encasement of inferior vena cava was found in 4 cases, the involvement of iliac artery in 3 cases and hydronephrosis in 9 cases.Conclusion IRPF demonstrated ultrasonic characteristics that would facilitate its detection and diagnosis.

This study was designed to discover filovirus entry inhibitors in a drug library of commercial medicines. One thousand and six hundred drugs were screened using the ZEBOV-GP/HIV model, a pseudovirus formed by an HIV-core packed with the Zaire Ebola virus glycoprotein. We identified 12 gonadal hormone drugs with inhibitory activities in ZEBOV-GP/HIV entry at final concentration of 10 μmol x L(-1). Among them, three drugs exhibited strong activities with IC50 < 1 μmol x L(-1), such as toremifene citrate (IC50: 0.19 ± 0.02 μmol x L(-1)), tamoxifen citrate (IC50: 0.32 ± 0.01 μmol x L(-1)) and clomiphene citrate (IC50: 0.53 ± 0.02 μmol x L(-1)); seven drugs had moderate activities with IC50 between 1 and 10 μmol x L(-1), such as estradiol benzoate (IC50: 1.83 ± 5.69 μmol x L(-1)), raloxifene hydrochloride (IC50: 3.48 ± 0.07 μmol x L(-1)), equilin (IC50: 4.00 ± 9.94 μmol x L(-1)), estradiol (IC50: 5.26 ± 9.92 μmol x L(-1)), quinestrol (IC50: 6.36?5.37 gmol-L1), estrone (IC50: 6.87 ± 0.03 μmol-L1) and finasteride (IC50: 9.94 ± 0.45 μmol x L(-1)); two drugs, hexestrol (IC50: 14.20 ± 0.55 μmol x L(-1)) and chlormadinone acetate (IC50: 24.60 ± 0.36 μmol x L(-1)), had weak activities against ZEBOV. Further, toremifene citrate, tamoxifen citrate, clomiphene citrate, raloxifene hydrochloride and quinestrol could block both pseudovirus type Sudan ebola virus (SEBOV-GP/HIV) and Marburg virus (MARV-GP/HIV) entries.
Antiviral Agents ; pharmacology ; Drug Evaluation, Preclinical ; Ebolavirus ; drug effects ; physiology ; Hemorrhagic Fever, Ebola ; Humans ; Marburgvirus ; drug effects ; physiology ; Selective Estrogen Receptor Modulators ; pharmacology ; Virus Internalization ; drug effects

Objective To study the clinical effect of tension-free hernia repair in inguinal emergency hernia. Methods The tension-free hernia repair was administered in 83 patients with inguinal emergency hernia.All the cases were analyzed retrospectively.Results There were two postoperative deaths in 83 cases within three days after operation.Wound infection occurred in one case.The rest recovered soon.The average stay in hospital was 5.1 days. Conclusion The tension-free hernia repair can be applied to inguinal emergency hernia with satisfactory results.

Objective:To investigate the relationship between dopamine D1 receptor ( DRD1 ) gene polymorphisms and clozapine efficacy in male schizophrenic patients. Methods:Totally 46 male schizophrenic patients were treated by clozapine for 6-8 weeks. The clinical response was determined by the Positive and Negative Symptom Scale (PANSS). DRD1 gene rs265981, rs5326, rs4532, rs1799914, rs686 and rs4867798 polymorphisms were detected by the gene sequencing, while plasma clozapine levels were monitored during the treatment. Results:The total clinical efficacy of clozapine response group and the non-response group was compared, the distribution of rs265981 genotype TT, TC and CC and allele T and C had statistically significant differences (P=0. 025;P=0. 005), and the distribution of rs686 genotype CC, CT and TC and allele C and T had statistically significant differences ( P=0. 044;P=0. 010). The negative symptom of the response group was compared with that of the non-response group, the distribution of rs4532 gen-otype GG, GA and AA and allele G and A had statistically significant differences (P=0. 034; P=0. 013). Conclusion: The poly-morphisms of DRD1 gene rs265981 and rs686 may have influence on the clinical efficacy of clozapine, and rs4532 may have influence on the negative symptom.

Objective To investigate the clinical characteristics, prognosis and treatment of pleural effusion of multiple myeloma. Methods From Jun 2005 to March 2010, of 162 patients with multiple myeloma treated in our department were complicated with pleural effusion, whose the proportion male to female was 8:7 and the median age was 56.8 (37-68) years. The diagnosis of malignant pleural effusion was dependent on physical examination, chest X ray, pleural aspiration and pleural fluid cytology. According to internatinal system stages and Durie-Salmon stages, the patients were staged. Results The results of pleural fluid cytology showed that the myeloma cells were positive in 13 patients and was negative in the other 2 patients. All patients received the chemotherapy regimen including bortezomib, large dose of glucocorticoids and cisplatin, cyclophosphamide, etoposide or prednisone (DECP). Although pleural effusion was controlled in some degrees, but were quickly relapsed. Eleven patients died, 2 of steady disease and 2 follow-up. Conclusion Multiple myeloma with myelomatous pleural effusion was very poor in the prognosis. Myelomatous pleural effusion was taken as a late manifestation in multiple myeloma or prediction of the aggressive behavior of the disease.

Objective To study the accuracy,clinical value and limit in assessing the vessels surrounding pancreas involved by pancneutic carcinoma with low field MRI.Methods MRI scanning was performed in 55 patients with pancreatic carcinoma.We prospectively evaluated the MRI findings of vascular involvement by pancreatic carcinoma,and compared with the surgical results.Results Of total 55 cases,50(91%)cases were correctly diagnosed on MRI .MRI carries an accuracy of 86% for prediction of resectility,and accuracy of 93% for unresectility.Conclusion Low field MRI can accurately assess the peripancreative vessels ,but it has limited value in the patients with pancreatic carinomaⅠa orⅠb.

This study is to investigate the mechanism of human promyelocytic leukemia HL-60 cells proliferation induced by alteronol in vitro. Human promyelocytic leukemia HL-60 cells cultured in vitro were treated with different concentrations of alteronol. Inhibition rate was detected by SRB assay. Cellular morphological changes were observed by Hoechst and AO/EB (acridine orange/ethidium bromide dye) staining. The apoptosis rate was determined by Annexin V-FITC/PI assay. Cell cycle distribution was determined by flow cytometry. Western blotting analysis was carried out to determine the cell cycle related proteins. The proliferation of HL-60 cells treated with alteronol was inhibited in a concentration-dependent manner. Based on cell viability assay, observation on cell morphology and apoptosis rate, it confirmed that alteronol played an obvious role in proliferation inhibition of human promyelocytic leukemia HL-60 cells, but it did not induce apoptosis in human promyelocytic leukemia HL-60 cells in different concentrations groups. Alteronol could effectively inhibit the proliferation of human promyelocytic leukemia HL-60 cells inducing cell cycle arrest at G1 phase, as well as, alteration expression of cell cycle proteins level of CyclinD1 and pRb.

Objective To investigate the expression of periostin in the kidneys of rats with obstructive nephropathy and its relevance to renal interstitial fibrosis.Methods Eighteen male adult SD rats were randomly divided into sham group,model group and benazepril group (6 in each group).Unilateral ureteral obstruction (UUO) model was induced by ligating the left ureter of rats.RT-PCR was used to detect the mRNA expressions of periostin and TGF-β1,and ELISA to detect the protein expression of periostin,Ang Ⅱ,and TGF-β1 in kidney tissue.Pathological changes of renal tissue were observed by HE and Masson staining.The protein expression of collagen Ⅰ (Col Ⅰ ) in kidney tissue was examined by immunohistochemical staining.Results The mRNA expressions of periostin and TGF-β1 in model group increased markedly as compared with sham group (all P＜0.05),and benazepril could decrease these mRNA expressions (all P＜0.05).The protein expressions of periostin,Ang Ⅱ and TGF-β1 in kidney tissue were significantly increased in model group as compared with sham group (all P＜0.05),and benazepril could decrease these protein expressions (all P＜0.05).The expression of periostin in kidney tissue was positively correlated with the expressions of Ang Ⅱ,TGF-β1 and Col Ⅰ,as well as the degree of renal interstitial fibrosis (r=0.652,0.781,0.776 and 0.825 respectively,all P ＜0.05).Conclusion Periostin expression is up-regulated in kidney tissue of rats with obstructive nephropathy,which is associated to the over-deposition of extracellular matrix (ECM) in the kidneys of UUO rats.