At present,research of genes and signal transduction pathway for etiology and pathogenesy of diseases is a hot topic.STAT3 signal transduction plays an important role in regulating cell proliferation,differentiation,survival and apoptosis.As a point of convergence for numerous oneogenie signaling pathways,STAT3 is constitutively activated in diverse human cancer cells.Current study suggest that activation of STAT3 signal transduction is closely related to the currence,development and malignant biological behaviors of tumor.STAT3 has been identified as a promising target for cancer treatment.

Books ; Medicine, Chinese Traditional ; Translations

Objective To detect supernatant of SD rat bone marrow mesenchymal stem cells(BMSC)and liver cells,and explore the role of cytokines,and provide a theoretical basis for the biological artificial liver and clinical treatment of liver failure.Methods BMSCs and primary hepatocytes of SD rat were isolated.BMSCs with its third generation and liver cells by the ratio of 1∶10 were co-cultured.Hepatocytes and BMSC were treated as control group.Observation the cells′survival and morphology and analysis of cytokine after 48 h were made by RayBiotech.Results BMSC-hepatocyte in the co-culture group grow and proliferated rapidly,and hepatocyte could survive up to 14 days;while hepatocyte cultured alone,it grow slowly and survived only 9 days.Expression of BM-SC-liver cells supernatant changed obviously:interleukin 1,interleukin 6,interleukin 10 were higher than those in the control group,above 2 times,which showed significant difference (P<0.01).Conclusion Co-culture of BMSC-liver cell could induce BM-SC secrete paracrine and autocrine:IL-1 and IL-6 and IL-10,which could promote the growth of hepatocyte and extend the liver cell survival time.In-vitro BMSC-liver cells could provide biological artificial liver cell sources,as well as provide theory basis for using cytokine treatment for liver failure.

Purpose]The purpose of this thesis is to briefly study how to translate different types of anadiplosis in Yellow Emperor's Canon of Internal Medicine, so as to evoke other translators'attention in this field. [Methods] There are different types of anadiplosis in Yellow Emperor's Canon of Internal Medicine, thus this thesis first summarizes the categories of anadiplosis structure in Yellow Emperor's Canon of Internal Medicine, then studies the respective use and mixed use of domestication and foreignization strategy in translation of the three types of anadiplosis structures. Furthermore, it also analyzes the advantage and disadvantage of domestication and foreignization strategy and the scope of their application. [Results] Through research, the thesis discovered that both domestication and foreignization strategy have their own advantage and disadvantage in translating anadiplosis in Yellow Emperor's Canon of Internal Medicine. On one aspect, translators should spread as much traditional medical culture as possible; on the other hand, translators should also take readers' response into accounts. Although domestication is a major trend nowadays, with the deepening of culture exchange, foreignization will play a more important role in TCM classics translation. [Conclusion] Domestication and foreignization strategy complement each other in the translation of anadiplosis in the Yellow Emperor's Canon of Internal Medicine. However, because of the requirement to spread traditional Chinese medicine, foreignization strategy will be used more frequently than before.

Objective To investigate the effect of conditional YY1 knockout in vascular endothelial cells on ovarian function in mice and its mechanism, so as to provide a new target and strategy for the diagnosis and treatment of infertility caused by angiogenesis disorder. Methods YY1~(flox/flox)(WT) and Cdh5-CreER~(T2)mice were constructed by CreER~(T2)/LoxP system, and then mated to obtain the YY1 vascular endothelial conditional knockout mice(Cdh5-CreER~(T2); YY1~(flox/flox), referred to as YY1~(i△EC)). The genotypes were identified by PCR. By administering tamoxifen to YY1~(iΔEC)mice to induce Cre enzyme activity,temporal-specific knockout of YY1 in vascular endothelial cells was achieved. After knockout for one month, the mouse pulmonary vascular endothelial cells were isolated, and the knockout efficiency was verified by immunofluorescence and Western blot. Using WT female mice as control, the YY1~(i△EC)female mice after knockout were detected for the fertility, estrous cycle and ovary angiogenesis, and observed for the ovary and uterus. Results After knockout of YY1 for one month, the number of vascular endothelial cells in YY1~(i△EC)mice decreased, and YY1 in vascular endothelial cells was knocked out efficiently and specifically. Compared with WT female mice, the ability of female YY1~(i△EC)mice to conceive and give birth to cubs was greatly reduced; the female YY1~(iΔEC)mice exhibited disrupted estrous cycles, and had spontaneous ovulation and impaired formation of corpus luteum; the ovarian angiogenesis was significantly inhibited(F = 1. 288, P < 0. 01); the ovary and uterus were small in size, and the ovary was pale in color and lacked corpus luteum on the surface. Conclusion Conditional YY1knockout in vascular endothelial cells can cause reproductive dysfunction in mice, which may be due to the inhibition of ovarian angiogenesis by vascular endothelial cell YY1 deficiency, resulting in impaired ovulation and luteinization function.

ObjectiveTo explore the relationship between β2-adrenergic receptor (β2-AR) gene Arg16Gly polymorphism and essential hypertension (EH) in elder (≥60 years old).Methods150 old healthy controls and 115 old patients with EH (all of them without kin relation) were selected to test genotype of β2-AR by the technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP).ResultsThe frequencies of three genotypes (Arg/Arg, Arg/Gly and Gly/Gly) were 22.81%, 53.51% and 23.68% in the EH group and 27.33%, 67.33% and 5.33% in the controls respectively. The frequencies of Arg and Gly allele were 49.56 % and 50.44% in the EH group, and 61.00% and 39.00% in the controls. There was a significant difference in distribution of alleles/genotypes between EH group and controls ( P<0.05).Conclusionβ2-AR Arg16Gly gene polymorphism is possibly associated with hypertension in this study population.

Objective To research the effect of GANT61 on epithelial‐mesenchymal transition (EMT) in human lung cancer H1703 and A549 cells lines ,and to preliminarily investigate its action mechanism .Methods DMSO was used as the control(DMSO group) .After treating H1703 and A549 cells with GANT61 for 24 h ,the gene changes of Gli‐1 ,Gli‐2 ,E‐cadherin and Vimentin were detected by using the real time fluorescence quantitative PCR method .The influence of GANT61 on the expression of E‐cad‐herin and Vimentin protein after acting on H1703 and A549 cells was observed by using the Western blotting assay .The scratch healing test was performed to evaluate the effect of GANT 61 on the tumor cell invasion ability after acting on H1703 and A549 cells .Results The real time fluorescence quantitative PCR showed that ,compared with the DMSO group ,GANT61 down‐regulated the mRNA expression of Gli‐1 ,Gli‐2 and Vimentin mRNA of H1703 and A549 cell lines and elevated the expression of E‐cadherin protein(P<0 .01);the Western blotting showed that GANT61 down‐regulated the expression of Vimentin of H1703 and A549 cell lines ,and elevated the expression of E‐cadherin(P<0 .01);the scratch healing test revealed the invasion ability of H 1703 and A549 cells in the GANT61 treatment group was significantly decreased (P<0 .01) .Conclusion EMT in lung cancer is related with aber‐rant activations of Gli1 and Gli2 in Hedgehog signal transduction pathway ,GANT61 could influence the EMT ability in lung cancer cells by down‐regulating the expression of Gli‐1 and Gli‐2 .Gli could become a new molecular target for inhibiting the lung cancer cell metastasis.

Neonatal necrotizing enterocolitis is a common gastrointestinal disease in neonate.Recently,with the establishment of the NICU and the all-round development of perinatal medicine and pediatrics,survival rate of premature especially very low birth weight neonates have obviously increased and the incidence of the disease is rising year by year.It is generally considered that multiple risk factors such as premature birth,low birth weight,infections,asphyxia,irrational use of drugs and so on,acting on the immature intestine,but the pathogenesis is still unknown.Therefore,it is significant to clearify the pathogenesis of the risk factors of NEC for studying the pathogenesis of the disease and providing prevention and control strategy.

AIM:To observe the effects of cornus iridoid glycosides(CIG)on inflammatory reaction especially the inflammatory cytokines in the brain after traumatic brain injury,and to explore the possible mechanisms of its neuroprotective effect.METHODS:SD rats were intragastrically administered with different doses of CIG(30,60 and 120 mg?kg-1?d-1)for 7 d.The traumatic brain injury rat model was induced by improved Feeney's fall weight method,and the brains were taken out 24 h and 72 h after brain injury,respectively.The morphological changes were observed by HE staining in the cerebral cortex.The expressions of inflammatory cytokine tumor necrosis factor-?(TNF-?)and interleukin-1?(IL-1?)were detected by immunohistochemical method.The image processing and statistical analysis were used to measure the number and the area of immunoreactive cells.RESULTS:HE staining showed the pathological changes were serious in the cerebral cortex of model group,and compared with the model group,the pathological changes were obviously reduced in CIG group.The positive immunoreactive cells of TNF-? and IL-1? were mainly distributed around the foci of contusion,the expressions of TNF-? and IL-1? in the model group were significantly higher than those in sham operated group,and the high expressions were sustained from 24 h to 72 h after brain injury.Compared with the model group,the levels of TNF-? and IL-1? in the brain of CIG treatment groups were obviously decreased in a dose-dependent manner and the inhibitory effects of TNF-? and IL-1? were more significant at 72 h after brain injury.CONCLUSION:CIG may have neuroprotective effect on traumatic brain injury through inhibiting the expression of inflammatory cytokines and reducing the inflammatory reaction.

At present, diagnostic standards and treatment guidelines for mental stress-induced myocardial ischemia have not been established.Clinical symptoms are often seen in patients, but clinical evidence of myocardial ischemia is lacking.With increasing research closely related to mental stress-induced ischemia and adverse cardiovascular outcomes, more and more attention has been paid to coronary microcirculation in stenosis or coronary microvasospasm.With mental stress as a starting point, this article analyzes the incidence and characteristics of mental stress-induced myocardial ischemia and reviews its pathological mechanisms from the perspectives of myocardial inflammatory reaction, vasoconstriction, endothelial dysfunction, sympathetic nerve conduction and genetic factors.