Child ; Coronary Aneurysm ; diagnostic imaging ; physiopathology ; Coronary Thrombosis ; diagnostic imaging ; physiopathology ; Coronary Vessels ; diagnostic imaging ; physiopathology ; Female ; Humans ; Mucocutaneous Lymph Node Syndrome ; complications ; diagnosis ; diagnostic imaging ; physiopathology ; Ultrasonography, Doppler

The expression of microRNAs (miRNAs) alters obviously in ovarian cancer.Through miRNA profile based on a microarray platform and further research on individual one,they are found to be closely related to pathogenesis,progression,recurrence,and drug resistance of ovarian cancer and have a good prospect in applying it in the early diagnosis,detection recurrence,prognosis and therapy of ovarian cancer.

Objective To investigate factors related to alexithymia of patients with asthma.Methods 52 patients with asthma were assessed with TAS-26,POMS-SF and EPQ-RSC.Results Compared with normal model control , patients with asthma showed alexithymia(t=2.15～5.29,P＜0.05～0.01). There was negative correlation between the total score of TAS,Ⅰ,Ⅱ, Ⅲ , Ⅳ and E(r=-0.372～-0.451,P＜0.01), between Ⅲ and N (r=-0.37,P＜0.01).There was opposite correlation between the total score of TAS,Ⅰ, Ⅱand N (r=0.312～0.415,P＜0.01),and between Ⅱ, Ⅳ and P(r=0.531～0.621,P＜0.01). There was close relation between alexithymia and mood state. There was opposite correlation between the total score of TAS,Ⅰ,Ⅱ, Ⅳ and the total score of POMS, TA,DD,AH,FI , CB(r=0.449～0.779,P＜0.01). There was negative correlation between the total score of TAS,Ⅰ,Ⅱand VA (r=-0.418～-0.544,P＜0.05～0.01),and between Ⅳ and VA(r=-0.320,P＜0.05).Conclusion Patients with asthma have alexithymia.There was close relation between alexithymia,personality traits and mood state.

Objective To investigate the morbidity rate and reason of ventricular fibrillation (Vf) undergoing select coronary angiography (SCA).Methods To analysis some correlated factors of Vf during SCA. Results 11 cases with Vf undergoing SCA, including 5 cases with double vessel disease, 6 patients with triple vessel lesion. Of 11 cases, 4 patients with left main coronary artery lesion were accompanied by more other branch lesions of coronary artery.Conclusion Vf is not infrequent in patient with multivessel lesion during SCA. It is important to observe coronary artery pressure and electrocardiogram change undergoing SCA.

Objective To evaluate the application of Snodgrass urethroplasty for failure hypospadias repairment.Methods Eighty-four cases of failure hypospadias were treated by Snodgrass urethroplasty from 2001 to 2005.The average age of patients was 3.7 years old.In the meantime,Duplay procedure was undertaken for other failure hypospadias with enough penile skin(76 cases).Results There were 64 cases cured,6 cases with urethral stricture and 14 cases with urethral fistula in 84 failure hypospadias cases.Sixty-one cases were cured in 76 cases used Duplay procedure.Conclusion Snodgrass is recommended for failed hypospadias cases with narrow urethral plate.

Objective To investigate clinical efficacy and safety of carboprost tromethamine injection combined with low B -Lynch suture for intractable postpartum hemorrhage placenta previa.Methods 125 cases of intractable postpartum hemorrhage placenta previa were divided into two groups according to treatment(n =62)with the observation group(n =63),the control group was administered oxytocin,and the line in the placental separation surface 8 interrupted suture;the observation group was used carboprost tromethamine injection combined with low B -Lynch suture.Clinical efficacy,blood loss,bleeding,postoperative bleeding 2h,24h after blood loss,transfusion rate and index sex hormone levels were compared before and after treatment.Results (1)After treatment,total effec-tive rate of the control group and observation group was 77.42%(48 /62)and 95.24%(60 /63)respectively.There was a statistically significant difference between the two groups(P <0.05);(2)Blood loss,bleeding time,2h after blood loss,blood loss and transfusion rate after 24h in the observation group were (1 608.37 ±43.01)mL,(8.89 ± 1.09)min,(101.18 ±8.73)mL,(221.17 ±20.58)mL and 4.76%,respectively,which were significantly lower than those of the control group [(1 992.28 ±55.62)mL,(12.83 ±1.77)min,(145.49 ±12.19)mL,(255.54 ± 23.30)mL and 16.13%](t =4.559,5.128,3.928,3.382,χ2 =5.559,all P <0.05);(3)before and after treatment indicator hormone(E2 ,FSH and LH)levels were not significantly different(P >0.05),and after treatment,the index level differences were not statistically significant(P >0.05);(4)the two groups during treatment,liver and kidney functions were normal,three cases of the control group and two cases of the observation group occured nausea and vomiting.Conclusion Carboprost tromethamine injection combined with low B -Lynch suture has significant effect, a small amount of bleeding,high security for intractable postpartum hemorrhage placenta previa,which should be promoted.

AIM: To investigate the expression pattern of apolipoprotein M (apoM) protein in renal cortex of a-cute renal failure ( ARF) rats with reperfusion. METHODS: Seventy - five male rats were randomly divided into sham operation group (re =25) , ARF group (n =25) and pyrrolidine dithiocarbamat (PDTC) group (n =25) , five subgroups at time points of 3 h, 6 h, 12 h, 24 h and 48 h after reperfusion were set up in each group. The expressions of apoM in cytoplasm and NF - κB p65 in nucleus of renal cortex were detected at the indicated time points. RESULTS: The expression of apoM in ARF group was obviously higher than that in sham operation group ( P <0.01 ) , and two peaks were detected, the first peak was at 6 h after reperfusion, while the second one was from 24 h to 48 h. The tendency of apoM expression in PDTC group was similar to that in ARF group, while the expression in every subgroup was prevalently lower than that in ARF group (P < 0.01). Otherwise, a significant correlation ( r = 0.852, P < 0.01) was found between the expression of apoM and NF -κB p65.CONCLUSION: The results indicate that apoM feasibly take part in the pathogenesis of ARF through the inflammatory reaction mediated by NF - κB.

Objective To investigate the relationship between the presence of circulating tumor cells (CTC) and the clinicopatho-logic characteristics of primary lung cancer patients .Methods Samples of peripheral blood(7 .5 mL) from 65 cases of patients with lung cancer(lung cancer group) and 10 cases of patients with lung benign diseases and 10 healthy volunteers (control group) ,and then those CTC were enriched by negative immunomagnetic separation with anti-CD45 antibody and then dectected by immunocyto-chemistry with anti-pan-cytokeratin antibody .The relationship between the positive rate of CTC and clinicopathologic characteristics of lung cancer patients were analyzed .Results The positive rate of CTC in lung cancer patients was 69 .23% (45/65) ,while CTC was not detected in any patients with lung benign disease or healthy volunteers .The positive rate of CTC in patients at TNM stageⅠ - Ⅱ ,Ⅲ and Ⅳ was 23 .08% (3/13) ,50 .00% (6/12) and 90 .00% (36/40) ,respectively .There was significant difference in posi-tive rate of CTC between patients at different TNM stages (P= 0 .000) .The positive rate of CTC in metastatic (stage M1 ) lung cancer patients(83 .33% ,35/42) was significantly higher than that in non-metastatic(stage M0 ) lung cancer patients(43 .48% ,10/23)(P=0 .002) .With the increasing number(0 ,1 ,2 ,3) of metastatic sites ,the positive rate of CTC increased (53 .48% ,83 .33% , 87 .50% ,75 .00% ) ,and there was significant difference in different positive rates of CTC (P=0 .024) .There was statistically sig-nificant difference in the positive rate of CTC between Cyfra 21-1 normal group(53 .33% ,16/30) and high value group(92 .00% ,23/25)(P=0 .021) ,the same with LDH normal group(55 .00% ,22/40) and high value group(82 .86% ,29/35)(P=0 .004) .Conclu-sion CTC were presented in some lung cancer patients .The presence of CTC in peripheral blood is correlated positively with the patients′clinical stage ,M stage ,the number of metastatic sites ,Cyfra21-1 value and LDH value .CTC detection is useful for more accurate risk assessment of patients with lung cancer .

Objective To detect the difference of the expression of HLA-DQA1 genes polymorphism in the lung cancer patients with pneumoconiosis and the non-occupational lung cancer patients.Methods Multiple polymerase chain reaction-ligase detection reaction classification method (PCR-LDR) was used to detect 6 alleles in HLA-DQA1 and the expression of HLA-DQA1 gene between the 21 cases of pneumoconiosis complicated with lung cancer and 40 cases of non-occupational lung cancer respectively.The correlation between them with pneumoconiosis complicated with lung cancer was analysed.Results HLA-DQA1 0301 gene frequencies were 19％ in pneumoconiosis complicated with lung cancer group and 2.5％ in non-occupational lung cancer group respectively,with a statistically significant difference (x2 =10.022,P =0.002).There was no statistically significant difference in the other allele between the two groups.Conclusion HLA-DQA1 gene polymorphism may be related to the susceptibility of the pneumoconiosis complicated with lung cancer.DQA1 0301 is one of the important genes of pneumoconiosis complicated with lung cancer,which maybe the risk factor for the susceptibility of the pneumoconiosis complicated with lung cancer.

Objective To investigate the mechanism of radiosensitizing effects of endostatin on H-520 human lung squamous cancer cells.Methods H-520 cells was treated with endostatin and/or radiation.Colony-forming assays were used to indicate the radiosensitising effects.Cell cycle distribution and expression of phosphor-p38-MAPK were assayed by FCM,and cyclin D1,cdk2,cdk4 and survivin mRNA leveh were assayed by RT-PCR.Phosphor-Akt was evaluated by Western-blotting.Results Combination of endostatin and irradiation inhibited the proliferation of H-520 cells.According to the colony-forming assays,the D0,Dq,D10 and SF2 values of the combination groups were much lower than those of irradiation groups.The sensitization enhancement ratio(SER)was 1.51.G2/M arrest occurred after 4 Gy irradiation.The gene expression of cyclin D1,cdk2,ckd4 and survivin and phosphor-Akt protein were down-regulated after treatment.The expression of phosphor-p38-MAPK protein was also down-regulated after treatment with 200 μg/ml endostar.Conclusions Endostatin inhibits the growth of H-520 cells and radiosensitizes the cells by induction of G0/G1 arrest,cell apoptosis and down-regulation of gene expression of cyelin D1,cdk2,cdk4 and reduces the phosphorylation of Akt and p38-MAPK.