Fracture healing is regulated by a complex array of factors,of which calcitonin gene-related peptide(CGRP) has been shown to play an anabolic role in bone metabolism.It has been reported that CGRP-containing sensory nerve fibers increased apparently at bone fracture site.CGRP is an important sensory neuropeptides expressed in bone tissue,although the primary function of this neuropeptides is to induce vasodilatation and blood flow,substantial evidence has shown that targeted expression of CGRP to mouse osteoblasts increased bone density,stimulated cancellous bone formation in vivo,especially the presence of CGRP receptors on rat osteoblast provides further evidence for a direct role for CGRP in modulating the function and growth of bone.CGRP is also proved to be a neuropeptide with proinflammatory activities,it may play a direct role in initiating the local inflammatory cell infiltration in the fracture site.Although it has been recognized that CGRP plays an important role in bone repair and reconstruction,the mechanism of this activation still remains to be elucidated.

Objective To investigate the effects of siRNA silencing of inhibitor of apoptosis protein gene on the proliferation of airway smooth muscle in bronchial asthma mice .Methods Tweenty female BALB/c mice were randomly divided into experimen‐tal group and control group .Mice in the experimental group were constructed asthma models .The trachea and bronchi were collect‐ed for cell culture .Respectively ,the cultured airway smooth muscle cells in the experimental group and the control group were grouped:PBS control group (added to PBS solution) ,missense chain control group (transfected with missense chain) and siRNA transfection group (transfected with siRNA Survivin) .The expressions of Survivin mRNA ,Survivin protein and caspase‐9 protein in airway smooth muscle cells were detected .The levels of IL‐6 and CCL5 in cell supernatant were detected .Results RT‐PCR re‐sults showed that ,the relative expression levels of Survivin RNA of airway smooth muscle cells in the siRNA transfection group of the experimental group were lower than the PBS control group and missense chain group ,the differences were statistically signifi‐cant (P<0 .05) .Western blot results showed that ,the expressions of Survivin proteins in the siRNA transfection group of the ex‐perimental group were lower than the PBS control group and missense chain control group ,while the caspase‐9 protein were higher , In the experimental group ,the synthesis and secretion levels of IL‐6 and CCL5 of airway smooth muscle cells in the siRNA transfec‐tion group were lower than the PBS control group and missense chain control group ,the cell proliferations at 2-4 d in the siRNA transfection group were lower than the PBS control group and missense chain control group ,the proportion of G0/G1 phase in the siRNA transfection group were higher than the PBS control group and missense chain control group ,but the proportion of S/G2 phase were lower than the PBS control group and missense chain control group ,cells apoptosis rates of the siRNA transfection group were lower than the PBS control group and missense chain control group ,the differences were statistically significant (P<0 .05) .Conclusion siRNA specific silencing Survivin gene could accelerate the airway smooth muscle cell apoptosis ,and inhibit cell abnormal proliferation and secretion .

Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality.Cases are reported in several countries in Africa,Europe,the Middle East,and Asia.Phylogenetic analyses based on the virus S (nucleocapsid),M (glycoprotein),and L (polymerase) genome segments sequences indicate distinct geographic lineages exist but their specific genetic characteristics require elucidation.In this work we collected all full length S segment sequences and generated a phylogenetic tree based on the alignment of these 62 samples.We then analyzed the alignment using entries from AAIndex,the Amino Acid Index database,to identify amino acid mutations that performed significant changes in charge,pka,hydropathy and side chain volume.Finally,we mapped these changes back to the tree and alignment to identify correlated mutations or sites that characterized a specific lineage.Based on this analysis we are able to propose a number of sites that appear to be important for virus function and which would be good candidates for experimental mutational analysis studies.

Objective:To detect periodontopathic bacterial DNA in atherosclerotic vascular lesions in a group of Chinese patients by using polymerase chain reaction(PCR) techniques.Methods: Ten human specimens of atherosclerotic plaque were obtained sterilely.The sclerotic lesions were blade into fine pieces and DNA was isolated from the samples.To detect Porphyromonas gingivalis(Pg),Tannerella forsythia(Tf),Aggregatibacter actinomycetemcomitans(Aa),Prevotella intermedia(Pi),Prevotella nigre-scens(Pn),Treponema denticola(Td),Campylobacter rectus(Cr),PCR amplification of bacterial 16S ribosomal DNA(rDNA) was carried out.Results: PCR assays for bacterial 16S rDNA indicated the presence of periodontal pathogens in 3 out of 10 surgical specimens.DNA of Pg were found in 3 samples,Tf was found only in one among the 3 samples and Pn was found in another sample among the 3 samples.Conclusion: The data confirm that DNA of periodontal pathogens present in atherosclerotic plaques.Pg,Tf,Pn may play a role in the development and progression of atherosclerosis in these Chinese patients.Further studies with large size samples are needed.

Objective To discuss the effect of psychological and behavioral intervention on sleep quality and self efficacy in patients with type 2 diabetes mellitus complicated with sleep disorder.Methods 80 patients with type 2 diabetes mellitus complicated with sleep disorder in our hospital were divided into control group (with 40 cases) and intervention group (with 40 cases)according to the random digital table method.The control group were given routine nursing,while the intervention group were given psychological and behavioral intervention based on the control group.The changes of sleep quality and self efficacy,scores of anxiety and depression and fasting blood glucose before and after intervention were compared between the two groups.Results The differences in the scores of PSQI and GESE were not statistically significant between the control group and the intervention group before intervention (P >0.05),which were significantly improved after intervention[(8.26 ±1.32)points,(29.65 ±3.01)points;(5.62 ± 1.03)points,(34.24 ±4.15)points;t =15.386,10.502,28.087,14.751,all P <0.05],and the scores in the inter-vention group were better than those in the control group (t =9.972,5.663,both P <0.05).There were not obvious differences in the scores of SAS and SDS between the control group and the intervention group before intervention(all P >0.05),which were significantly decreased after intervention[(41.24 ±3.21)points,(42.52 ±3.01 )points;(32.36 ±2.12)points,(34.24 ±4.15)points;t =19.674,12.550,27.589,19.546,all P <0.05)],and the scores in the intervention group were significantly lower than those in the control group (t =14.599,10.215,both P <0.05).There was no obvious difference in fasting blood glucose between the two groups before intervention (P >0.05),which were significantly decreased after intervention[(8.76 ±1.06)mmol/L,(6.25 ±1.32)mmol/L,t =3.646,8.955,all P <0.05],and the level in the intervention group was significantly lower than that in the control group (t =9.377,P <0.05).Conclusion The psychological and behavioral intervention can effectively improve the
sleep quality of patients with type 2 diabetes mellitus complicated with sleep disorder,improve the self efficacy,and relieve anxiety and other negative emotions,then decrease the fasting blood glucose level,which has promotion value in the clinical nursing.

Objective To evaluate the role of postoperative electron beam radiotherapy in the management of ke-loids. Methods Between January 2004 and December 2006,116 cases of keloids were treated with 6 MeV electron beam radiotherapy within 24 h after surgical resection of the keloids. All patients had received a total of 21 Gy in seven daily 3-Gy fractions. Treatment was started at the 24th h after surgery. Patients were followed up and the information regarding treatment results, early and late radiation toxicity,and the satisfaction level by self-assessments was recorded. Results Recurrence occurred in 17 cases of keloids(14. 7%),and was correlated with site of the lesions(χ~2 =29. 91,P<0. 01). Most recurrences were observed at site of sternum (10/43) and shoulder (5/13 ). Keloid associated symptoms, e. g. itching and pain, were improved in 88. %%. For early toxicity outcomes, 100% had grade 1～2 skin erythema,7. 8% had wound delayed union,and 4. 3% had infection. For late toxicity outcomes, 30. 2% reported grade 1～2 hyperpigmentation, 11. 2% grade 1～2 hypopigmentation, and 5. 2% grade 1 telangiectasia. No severe complications or secondary malignancies were observed. 72. 4% patients described the results of treatment as excellent or good,and 15. 5% patients were not satisfied with the treatment results. Conclusion Postoperative electron radiotherapy is well tolerated and effective in preventing keloid recurrence.

BACKGROUND:There are usualy removable appliances and fixed appliances in the mouth of orthodontic patients, resulting in periodontitis. Because of its high security, good heat resistance, long action time, not easy to produce resistance and wide antimicrobial spectrum, inorganic antibacterial agents have become a research hotspot. OBJECTIVE:To review the application and research progress of inorganic nano-antibacterial materials in orthodontic treatment. METHODS:A computer-based search of PubMed and CNKI databases was performed for articles about applications of inorganic nano-antibacterial materials in orthodontic treatment published from January 2001 to December 2014 using the keywords of “orthodontic, antibacterial agent” in English and Chinese, respectively. RESULTS AND CONCLUSION:Inorganic nano-antibacterial materials for oral bacteria have good antibacterial properties, and are a kind of ideal biological material. Bracket enamel adhesive, removable appliance resin material and bracket can play correct and antibacterial roles by modification of inorganic nano-antibacterial materials, so as to reduce complications such as dental caries. However, the application of nano-antibacterial materials is stil in its infancy, the modified materials need to be studied further in terms of color problems, physical and chemical properties and biological security.

Objective:To study on the levels of CXCL8 and its receptors (CXCR1 and CXCR2) in peripheral blood neutrophils of the patients with chronic hepatitis B.Methods:The neutrophils were isolated and purified by neutrophil isolation medium,and the loads of HBV-DNA in neutrophils were detected by PCR,and the levels of HBeAg in serum were measured by ELISA.The patients were divided into different groups according to the detective results so that the expressions of CXCL8 and its receptors ( CXCR1,CXCR2) in neutrophils were detected by the methods of streptavidin-biotin complex ( SABC ) immunocytochemistry stain.Results:The data of SABC immunocytochemical stain showed that the positive color of CXCL8 was mainly located in the cytoplasm of PMNs.However,the most positive color of CXCR1and CXCR2 was mainly expressed in the cytoplasm and cell membrane.Interestingly,the deeper immune coloring of CXCL8 and CXCR1, and relatively shallow immune coloring of CXCR2 were explored in the group with positive of HBeAg.The similar detective results also had been found in the cases with positive of HBV DNA in neutrophils.Compared with the normal control group,the levels of CXCL8 and CXCR1 in the patients were significantly increased ( P<0.05) ,but the differences were too small to be statistically significant in the level of CXCR2 (P>0.05).Conclusion:After neutrophils occult infected by HBV,not only the secretion of CXCL8 can be promoted, but also the expression of CXCR1 will be further increased.The data of immunohistochemical staining have been shown that the color degree of CXCL8 and its receptors ( CXCR1, CXCR2 ) are positive correlation to the level of HBeAg and the loads of HBV DNA.More PMNs can be chemotactic attraction to lesion so as to participate in the local inflammatory injury and tissue repair via the interactive pathway of the high expression of CXCR1 on surface of neutrophils with CXCL8.

Objective To analyse CT appearances of fibroma and fibrosarcoma and to provide evidences for clinical diagnosis and treatment.Methods 8 cases with surgically-and pathologically-proved hard fibroma(nasal cavity,maxillary sinus and soft palate each and all 2 cases)and fibrosarcoma(nasal cavity and maxillary sinus each and all 1 case)were studied with retrospectively analysis.The scanning parameters were 5 mm thickness and interval for axial scanning.Results CT appearances of fibroma were single circular,semicircular or elliptical soft tissue masses which usually had homogeneous density,clear margin,thin and full envelope,wide base or a pedicel and slight or middle enhancement after contrast.The tumors grew distensibly,bones near the mass were constricted and thinned or absorbed but never destroried and the structures nearby were deviated but not involved.Fibrosarcoma showed such common CT manifestations as other malignant tumors in the same locations.Conclusion CT appearances of fibroma has some features,they are different from more common benign lesions.Fibrosarcoma can not be differeciated from other maglinant tumors,thier diagnosis must depend on pathology.

Objective To discuss the application value of the treatment protocol of combining warm needling and atorvastatin in treating female cardiac syndrome X (CSX). Method Eighty female CSX patients were randomized into a control group and an observation group by the random number table, 40 cases in each group. The control group was intervened by atorvastatin in addition to the conventional treatment protocol, while the observation group was by warm needling plus atorvastatin in addition to the conventional treatment. The treatment efficacies and adverse events rates were compared between the two groups. Result There were no significant between-group differences in comparing the component and total scores of the Cardiac Anxiety Questionnaire (CAQ), NO, plasma endothelin (ET-1) and hypersensitive C-reactive protein (hs-CRP) levels before the intervention (P>0.05);after 8-week treatment, the component and total scores of CAQ, ET-1, and hs-CRP levels were significantly lower in the observation group than in the control group, and NO level was significantly higher in the observation group than in the control group (P<0.05). Meanwhile, at the end of the 8-week treatment, the total effective rate of the observation group was significantly higher than that of the control group (P<0.05). Besides, there was no significant difference in comparing the adverse events rate between the two groups during the 8-week treatment (P>0.05). Conclusion In the treatment of female CSX, warm needling plus atorvastatin can enhance the treatment efficacy.