Objective To investigate the turnover and factors influencing amniotic membrane transplantation into corneal stroma.Methods Amniotic membrane stained with dichlorotriazinyl aminofluoresclin (DTAF)was implanted into the corneal stroma of 1 5 New Zealand white rabbits.After transplantation,we observed the clinical changes.The tissue samples from grafted area were observed with HE staining and laser confocal microscope to trace amniotic membrane at 4,8,13,1 7 and 22 week after surgery.And the tissue samples at week 22 after surgery from grafted area were also observed by transmission electron microscopy.Results After 4 weeks of operation, amniotic membrane tissue bent with visible multi-layer homogeneous red dye.The structure remained intact;basement membrane and stroma were no dissolved.After 8 weeks, amniotic tissue was a strip structure of homogeneous red dye,and the substance layer was complete.After 13 weeks,amniotic membrane morphology appeared fuzzy,but still took the shape of strips;a small number of corneal cells accumulated around the amniotic membrane.At 1 7 weeks after operation,fragmented amnion was evident.At 22 weeks after operation,small pieces of amnion tissue remained in the corneal stroma.Laser scanning confocal microscopy revealed that amniotic membrane could retain for over 22 weeks in the corneal stroma.After 22 weeks,amnion stroma collagen fibers that were integrated into the corneal stroma were irregular.Corneal stromal ultrastructure surrounding the amniotic membrane did not obviously change.Conclusion Retention time of amniotic membrane transplanted into corneal stroma can be as long as over 22 weeks and amniotic membrane can be merged into the corneal tissue as a substitute, which wins time for corneal transplantation operation.

D-D4FC (?-D-2′,3′-didehydro-2′,3′-dideoxy-5-fluorocytidine),a new anti-HIV drug,is on its PhaseⅡ clinical trials in America,France and Germany. Our lab has synthesized D-D4FC successfully using N-deoxyribosyltransferase from Lactobacillus helveticto catalyzing the ribose transfer from D4T (?-D-2′,3′-unsaturated thymidine) to 5-FC (5-fluorocytidine).The yield of D-D4FC reached 25%.We discovered the reaction could also be done by using intact cells.The yield could increase to 50% in 12.5 hours and more convenient to industrial continuous process.In this paper,the conditions including pH,buffer,substrates concentration,cells amount,reaction time and a possible catalytic mechanism were studied and discussed.

Objective: Under the background of pension combined with medical service, for the elders to reasonably, effectively and fully utilize the medical resources, it is of vital significance to establish good medical con-sumptive behavior and consumption intention, to promote healthy old age and improve elders' health and happiness to help them cultivate a fine habit in their practical life. Methods: Based on the micro-survey data of investigation in Nanjing, this paper systematically analyzed the factors influencing elders' medical consumption behavior by using the theory of planned behavior and structural equation model. Results:Different factors were classified into behavioral at-titudes, subjective norms and perceived behavior control, and significant variables were found out through behavior model as well as though measuring their relative effects. Conclusion: There is no significant casual relationship be-tween the perceived behavior control and medical consumption behavioral intention. The elders' medical consumption behavior intention is affected by various social network relationships. The belief strength, normative belief, behavioral attitudes and subject norms can affect elders' medical consumption behavior.

Humans ; Male ; Prostatic Hyperplasia ; complications ; Quality of Life ; Urinary Bladder, Overactive ; complications

Study of traditional Chinese medicine (TCM) syndromes is a key to the research of TCM modernization, and the core is the classification and diagnostic criteria of syndromes. The purpose of this article is to review the usage of classification algorithms of data mining in TCM syndrome researches, and comprehensively analyze the main features of algorithms and their applications. The appropriate classification algorithm should be chosen according to different research purposes. Rough sets and cluster analysis are suitable for exploratory research without requiring a prior knowledge. Fuzzy sets theory, neural networks and decision tree are suitable for syndrome diagnostic criteria research when the classification goal is clear, because they require a prior knowledge. Among them, fuzzy sets theory could be used in combination with other classification algorithms. Thus, some new methods such as fuzzy clustering, fuzzy rough sets or fuzzy decision tree might be more suitable for TCM algorithm classification research. It is suggested that some novel classification algorithms need to be developed to fit the condition of TCM syndrome, based on the interdisciplinary theories and technologies.

Child ; Esophagus ; pathology ; Foreign Bodies ; diagnosis ; Humans

Objective To evaluate the outcomes of endoscopic sinus surgery (ESS) for children with chronic rhinosinusitis, who had no response to medication. Methods A total of 112 children (aged 12 to 17 years) with chronic rhinosinusitis caused by abnormal anatomy of the nose cavity were treated by ESS in our hospital. Septorhinoplasty was performed on the patients under an endoscope. Conchoplasty, adenoidectomy, or nasal polypectomy was performed simultaneously if necessary. Results The patients were followed up for 6-23 months (mean 13 months). The symptoms of rhinosinusitis disappeared in 110 patients (98.2%); whereas the other 2 patients showed no obvious improvement after the operation. Conclusions By using microinvasive ESS combined with conchoplasty, children with rhinosinusitis caused by abnormal anatomy that does not respond to medication could be cured.

Adult ; Humans ; Male ; Occupational Diseases ; Pulmonary Aspergillosis

Objective To study the effects of different concentration of interleukin(IL)-17 on the expression of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprogeterin (OPG) in human periodontal ligament fibroblasts (HPDLF) and explore the relationship between IL-17 and orthodontic root resorption.Methods HPDLF cell line was established through the tissue pieces culture method in vitro.HPDLF were stimulated by IL-17 with five different concentrations(0,5,10,20,40 ng/mL) for 24 h.The expression level of mRNA and protein of RANKL and OPG in HPDLF were detected by RT-PCR and ELISA,respectively.Results HPDLF expressed RANKL and OPG in 0 ng/mL group.The expression amount of mRNA and protein of RANKL in HPDLF was positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.The expression amount of mRNA and protein of OPG was negative correlation with the concentration of IL-17 in 5 to 20 ng/mL group.The ralative RANKL/OPG ratio of mRNA and protein were positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.Conclusion HPDLF expresses RANKL and OPG in the absence of IL-17.IL-17 enhances the expression of RANKL and inhibits the expression of OPG in HPDLF.

Objective To study the relationship between the health?related quality of life and Graves′ophthalmopathy quality of life(GO?QOL)in Graves′ophthalmopathy(GO)patients. Methods A total of 44 GO patients were recruited for the study and investigated with SF?36 and GO?QOL questionnaires. In addition,the thyroid function and related antibodies were also detected. Results The scores of SF?36 and GO?QOL were higher in the patients over 45 years than those under 45 years(P<0.05). GO?QOL score was lower in the patients with thyroid stimulating hormone recep?tor antibody higher than 10 IU/mL(P<0.05). The scores of SF?36 and GO?QOL were obviously higher in the patients who had a clinical activity score above 3(P<0.01). Conclusion GO?QOL is a better and more specific questionnaire for GO patients.