1.A case of Second Malignant Neoplasm Complicating Hodgkin's Disease in Remission.
Hong Hoe KOO ; Jong Woon CHOI ; Sang Oh NA ; Il Soo HA ; Hee Young SHIN ; Hyo Seop AHN ; Yeon Lim SUH ; Chul Woo KIM ; Je Geun CHI
Journal of the Korean Pediatric Society 1990;33(4):564-572
No abstract available.
Hodgkin Disease*
2.Intramedullary Clear Cell Ependymoma in the Thoracic Spinal Cord: A Case with Its Crush Smear and Ultrastructural Findings.
Na Rae KIM ; Dong Hae CHUNG ; Sang Koo LEE ; Seung Yeon HA
Journal of Korean Medical Science 2007;22(Suppl):S149-S153
Clear cell ependymoma was included in the World Health Organization classification of the nervous system in 1993, and all the reported cases, except for two in the spinal cord, were located in the brain, mainly in the supratentorial compartment. Astrocytomas outnumber ependymomas in the spinal cord, and the two entities partly share cytologic findings such as long, bipolar glial processes and oval to round nuclei resembling those seen in pilocytic astrocytoma. Here, we report the first Korean case of intramedullary clear cell ependymoma of the spinal cord, which is the third case situated in the spinal cord in the literature. The crush smear revealed round-to-oval nuclei with occasional nuclear eosinophilic inclusion and rare nuclear grooves. Cytoplasm had fluffy eosinophilic glial processes, and acellular fibrillary zone. On hematoxylin-eosin stain, oval to round tumor cells had large central nuclei with indistinct nucleoli and a moderate amount of clear cytoplasm, i.e. perinuclear halo, mimicking oligodendroglioma. Perivascular pseudorosettes and ependymal clefts were rarely found. In retrospect, perinuclear halo was absent on crush smears. Ultrastructurally, they had extensive surface microvilli and edematous cytoplasm filled with abundant glial filaments and microlumens with or without microvilli. Intercellular long cell junctions of the zipper-like zonula adherens type were found.
Aged
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Ependymoma/metabolism/*pathology/ultrastructure
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Female
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Humans
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Immunohistochemistry
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Microscopy, Electron, Transmission
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Spinal Cord Neoplasms/metabolism/*pathology/ultrastructure
3.Permeability Parameters Measured with Dynamic Contrast-Enhanced MRI: Correlation with the Extravasation of Evans Blue in a Rat Model of Transient Cerebral Ischemia.
Hyun Seok CHOI ; Sung Soo AHN ; Na Young SHIN ; Jinna KIM ; Jae Hyung KIM ; Jong Eun LEE ; Hye Yeon LEE ; Ji Hoe HEO ; Seung Koo LEE
Korean Journal of Radiology 2015;16(4):791-797
OBJECTIVE: The purpose of this study was to correlate permeability parameters measured with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) using a clinical 3-tesla scanner with extravasation of Evans blue in a rat model with transient cerebral ischemia. MATERIALS AND METHODS: Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil. DCE-MRI was performed 12 hours, 18 hours, and 36 hours after reperfusion. Permeability parameters (K(trans), v(e), and v(p)) from DCE-MRI were calculated. Evans blue was injected after DCE-MRI and extravasation of Evans blue was correlated as a reference with the integrity of the blood-brain barrier. Correlation analysis was performed between permeability parameters and the extravasation of Evans blue. RESULTS: All permeability parameters (K(trans), v(e), and v(p)) showed a linear correlation with extravasation of Evans blue. Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001). CONCLUSION: Permeability parameters obtained by DCE-MRI at 3-T are well-correlated with Evans blue extravasation, and K(trans) shows the strongest correlation among the tested parameters.
Animals
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Blood-Brain Barrier/pathology
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Capillary Permeability
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Contrast Media
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Disease Models, Animal
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Evans Blue/analysis
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Ischemic Attack, Transient/*diagnosis
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Magnetic Resonance Imaging/instrumentation/*methods
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Male
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Rats
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Rats, Sprague-Dawley
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Stroke/diagnosis
4.The Present Status and Perspective of Nationwide Occupational Diseases Surveillance System.
Seong Kyu KANG ; Young Koo JEE ; Yeon Soon AHN ; Hyoung Ok KIM ; Mi Na HA ; Ho Jang KWON ; Nam Jong PAIK ; Seong Ah KIM ; Yun Chul HONG ; Jai Yong KIM ; Dae Hee KANG ; Hye Sun JUNG ; Eun Hee HA
Korean Journal of Occupational and Environmental Medicine 2001;13(2):116-126
No abstract available.
Occupational Diseases*
5.Development of In Vitro Co-Culture Model to Mimic the Cell to Cell Communication in Response to Urban PM2.5
Yoon Jin ROH ; Hyun Ha NOH ; Na Yeon KOO ; Sun Hye SHIN ; Mi-Kyung LEE ; Kui Young PARK ; Seong Jun SEO
Annals of Dermatology 2022;34(2):110-117
Background:
Airborne particulate matter (PM), a widespread air contaminant, is a complex mixture of solids and aerosols composed of particles suspended in the air. PM is associated with inflammatory responses and may worsen inflammatory skin diseases. However, the mechanisms through which PM affects atopic dermatitis (AD) remain unclear.
Objective:
To establish an In Vitro model that more accurately mimics AD using human keratinocyte (HaCaT), dermal fibroblast (HDF), and mast cell (HMC-1) and using this model to investigate the mechanism through which PMs affect AD.
Methods:
An AD-like In Vitro model was established by seeding HaCaT, HDF, and HMC-1 cells with recombinant human interleukin (IL)-1α and polyinosinic:polycytidylic acid.We confirmed the effect of PM on the inflammatory cytokine expression of a triple-cell culture model. SRM 1649b Urban Dust, which is mainly composed of polycyclic aromatic hydrocarbons, was used as the reference PM. The effects of PM on the expression levels of proinflammatory cytokines and skin barrier markers were assessed using quantitative real-time polymerase chain reaction and western blotting. Inflammatory cytokine levels were measured using an enzyme-linked immunosorbent assay.
Results:
Interactions between various skin cell types were evaluated using a co-culture system. PM treatment increased mRNA and protein levels of the inflammatory cytokines IL-6, IL-1α, tumor necrosis factor-α, IL-4, and IL-1β and decreased the expression of the skin barrier markers filaggrin and loricrin.
Conclusion
Our results suggest that an In Vitro triple-cell culture model using HaCaT, HDF, and HMC-1 cells may be reliable for obtaining more physiological, functional, and reproducible data on AD and skin barriers.
6.Neurologic complications in patients with carotid artery stenting
Na Young KIM ; Jong Wook CHOI ; Kum WHANG ; Sung Min CHO ; Youn Moo KOO ; Jong Yeon KIM
Journal of Cerebrovascular and Endovascular Neurosurgery 2019;21(2):86-93
OBJECTIVE: Carotid artery stenting is helpful in patients with carotid artery stenosis and is a common method of treatment. However, data on the neurological consequences that might arise from, especially Asian patients after CAS is not enough. The purpose of this study was to investigate the outcome and prognostic factors affecting CAS patients.METHODS: From January 2013 to June 2018 it was enrolled 97 patients who underwent CAS with severe carotid artery stenosis in a single institution. We retrospectively reviewed neurologic complications such as restenosis, ipsilateral or contralateral stroke, and hyperperfusion during the 6-month follow-up period.RESULTS: There were no complication occured during the procedure in all 97 patients. Neurologic complications occurred in 30 patients (30.9%) after the procedure, and ipsilateral stroke 6 (6.2%), contralateral stroke 9 (9.4%), restenosis 2 (2.1%) and hyperperfusion 13 respectively. One of them had died (1.0%), of which the rest were discharged after symptoms improve. On univariate analysis, DM and pre-op NIHSS score was associated with the risk of CAS complication, exclusively. On the binary logistic regression for risk factors, DM (OR 0.144, 95% CI [0.029–0.718]), history of radiotheraphy (OR 36.103, 95% CI [1.009–1291.789]) and preoperative NIHSS (OR 1.266, 95% CI [1.059–1.513]) showed independent risk factors associated with post procedural neurological complications, statistically.CONCLUSION: Carotid artery stenting is a relatively safe and reliable long-term outcome for patients with carotid artery stenosis. However, careful observation should be taken after procedure immediately for any possible complications.
Asian Continental Ancestry Group
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Carotid Arteries
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Carotid Stenosis
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Endovascular Procedures
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Follow-Up Studies
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Humans
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Logistic Models
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Methods
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Postoperative Complications
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Retrospective Studies
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Risk Factors
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Stents
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Stroke
7.Two-signal blockade with anti-CD45RB and anti-CD154 monoclonal antibodies inhibits graft rejection via CD4-dependent mechanisms in allogeneic skin transplantation.
Eun Young KIM ; Eun Na LEE ; Jienny LEE ; Hae Jung PARK ; Chi Young CHANG ; Da Yeon JUNG ; Su Young CHOI ; Suk Koo LEE ; Jae Won JOH ; Sung Joo KIM
Experimental & Molecular Medicine 2006;38(3):284-294
Blockade of signal 1 or 2 for T-cell activation by the use of anti-CD45RB and anti-CD154 monoclonal antibodies (mAb) (two-signal blockade) has been proven effective in preventing or delaying graft rejection. However, the mechanisms of its immunomodulatory effects are clearly unknown and the present studies were performed to determine how the two-signal blockade modulate allogeneic immune responses, especially T-cell mediated cellular immunity, in a murine skin allograft model. We now report on the profound inhibition of alloreactive T cells by two-signal blockade via CD4-dependent mechanisms. C57BL/6 mice of BALB/c skin allograft were treated with anti-CD45RB, anti-CD154, CTLA4-Ig, or their combinations. For depletion of CD4 or CD8 T cells, the recipients received CD4-depleting or CD8-depleting mAb. We confirmed that survival of skin allograft was markedly prolongated in the two-signal blockade-treated group. In depletion study, anti-CD45RB, anti-CD154 and CD4-depleting mAb-treated group showed acute rejection of skin allograft in contrast to CD8-depleting group treated with the two-signal blockade. In the group treated with the two-signal blockade, the proportions of CD4+CD45RB(low)and CD8+CTLA-4 regulatory T cells were increased while effector CD8+ T cells, including IFN-gamma-secreting and CD8+CD62L(low)T cells, were decreased when compared with non-treated group. In contrast, the CD4-depleted group treated with the two-signal blockade resulted in recovery from immunoregulatory effects of two-signal blockade. In addition, results of IL-4 and IL-10 production were also showed CD4-dependence. Therefore, the two-signal blockade is accompanied by CD4-dependent mechanisms in allogeneic skin transplantation.
Transplantation, Homologous
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T-Lymphocytes, Regulatory/cytology/immunology
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Skin Transplantation/*immunology
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Signal Transduction/drug effects/immunology
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Mice, Inbred C57BL
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Mice, Inbred BALB C
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Mice
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Male
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Lymphocyte Depletion
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Lymphocyte Activation/immunology
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Interleukin-4/biosynthesis
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Interleukin-10/biosynthesis
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Graft Rejection/*immunology/prevention & control
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Flow Cytometry
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Cytotoxicity, Immunologic/immunology
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CD8-Positive T-Lymphocytes/cytology/immunology/metabolism
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CD40 Ligand/*immunology
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CD4-Positive T-Lymphocytes/cytology/immunology/metabolism
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Antigens, CD45/*immunology
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Antigens, CD4/*immunology
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Antibodies, Monoclonal/administration & dosage/*pharmacology
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Antibodies, Blocking/administration & dosage/pharmacology
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Animals
8.The Survival Rate of Korean Patients with COPD with or without Acute Exacerbations.
Soo Taek UH ; Ji Yeon LEE ; So Mi KOO ; Yang Ki KIM ; Ki Up KIM ; Jong Sook PARK ; Sung Woo PARK ; An Soo JANG ; Do Jin KIM ; Jae Sung CHOI ; Joo Ok NA ; Ki Hyun SUH ; Yong Hoon KIM ; Choon Sik PARK
Tuberculosis and Respiratory Diseases 2011;70(6):474-481
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by air flow limitation, which is one of the leading causes of mortality worldwide. There have been many studies on survival rates in the world literature, but there have been few reports regarding the survival rate in Korean patients with COPD. Acute exacerbation is regarded as a risk factor for mortality in patients with COPD. The purpose of this study was to investigate the survival rate and the effect of acute exacerbations on the survival rate of Korean patients with COPD. METHODS: A total of 502 COPD patients who were diagnosed on the basis of history and lung function tests were enrolled in this study. The frequency of acute exacerbations, body mass index (BMI), C-reactive protein (CRP) and pulmonary hypertension were analyzed. RESULTS: The 3- and 5-year survival rates were 98% and 83%, respectively. The median survival time was 78 months. The median survival time was 55 months in 322 patients with one or more acute exacerbations. The 3- and 5-year survival rates were significantly lower in the 322 patients with one or more acute exacerbations than in those without any. The mortality rate was significantly higher in patients with CRP >3 mg/L than in those with CRP < or =3 mg/L (p<0.005); it was significantly higher in patients with pulmonary hypertension than in those without it (p<0.01). CONCLUSION: Because the 5-year survival rate is 83% in Korean patients with COPD, the management of stable patients with COPD should focus on the prevention of acute exacerbations.
Body Mass Index
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C-Reactive Protein
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Disease Progression
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Humans
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Hypertension, Pulmonary
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Lung Diseases
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Pulmonary Disease, Chronic Obstructive
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Respiratory Function Tests
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Risk Factors
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Survival Rate
9.Distribution of Yeast and Mold Species Isolated from Clinical Specimens at 12 Hospitals in Korea during 2011.
Eun Jeong WON ; Jong Hee SHIN ; Won Kil LEE ; Sun Hoe KOO ; Shine Young KIM ; Yeon Joon PARK ; Wee Gyo LEE ; Soo Hyun KIM ; Young UH ; Mi Kyung LEE ; Mi Na KIM ; Hye Soo LEE ; Kyungwon LEE
Annals of Clinical Microbiology 2013;16(2):92-100
BACKGROUND: The incidence of fungal infections varies among hospitals and between different time periods. We performed a nationwide survey in Korea to investigate the distribution of yeast and mold species recovered from clinical specimens. METHODS: The distributions of clinical isolates of yeast and mold species obtained from 12 university hospitals between January and December 2011 were evaluated relative to the hospital and specimen type. RESULTS: A total of 39,533 fungal isolates (37,847 yeast and 1,686 mold isolates) were obtained. C. albicans was the predominant species (49.4%) among the yeast isolates from all clinical specimens, followed by C. glabrata (7.2%) and C. tropicalis (6.5%). For 5,248 yeast isolates from sterile body fluids, blood was the most common source of yeasts (71.1%), followed by peritoneal fluid (9.4%). Although C. albicans was the predominant species at all but two hospitals, the rate of non-albicans Candida species varied from 71.2% to 40.1%, depending on the hospital. The yeast species recovered most frequently from the sterile body fluids was C. albicans (41.7%), followed by C. parapsilosis (17.8%) and C. glabrata (14.4%), while that from non-sterile sites was C. albicans (50.7%), followed by C. glabrata (6.0%) and C. tropicalis (5.5%). For mold-forming fungi, Aspergillus species (62.3%) were most common, followed by Trichophyton species (15.4%). Respiratory specimens were the most common source of molds (39.6%), followed by abscesses/wounds (28.4%) and tissues (17.5%). CONCLUSION: The rank order of distribution for different fungal species varied among hospitals and specimen types. Continual national surveillance programs are essential for identifying possible changes in fungal infection patterns.
Ascitic Fluid
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Aspergillus
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Body Fluids
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Candida
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Fungi
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Hospitals, University
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Incidence
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Korea
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Trichophyton
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Yeasts
10.The Korean Organ Transplantation Registry (KOTRY): an overview and summary of the kidney-transplant cohort
Hee Jung JEON ; Tai Yeon KOO ; Man Ki JU ; Dong-Wan CHAE ; Soo Jin Na CHOI ; Myoung Soo KIM ; Jung-Hwa RYU ; Jong Cheol JEON ; Curie AHN ; Jaeseok YANG ;
Kidney Research and Clinical Practice 2022;41(4):492-507
As the need for a nationwide organ-transplant registry emerged, a prospective registry, the Korean Organ Transplantation Registry (KOTRY), was initiated in 2014. Here, we present baseline characteristics and outcomes of the kidney-transplant cohort for 2014 through 2019. Methods: The KOTRY consists of five organ-transplant cohorts (kidney, liver, lung, heart, and pancreas). Data and samples were prospectively collected from transplant recipients and donors at baseline and follow-up visits; and epidemiological trends, allograft outcomes, and patient outcomes, such as posttransplant complications, comorbidities, and mortality, were analyzed. Results: From 2014 to 2019, there were a total of 6,129 registered kidney transplants (64.8% with living donors and 35.2% with deceased donors) with a mean recipient age of 49.4 ± 11.5 years, and 59.7% were male. ABO-incompatible transplants totaled 17.4% of all transplants, and 15.0% of transplants were preemptive. The overall 1- and 5-year patient survival rates were 98.4% and 95.8%, respectively, and the 1- and 5-year graft survival rates were 97.1% and 90.5%, respectively. During a mean follow-up of 3.8 years, biopsy-proven acute rejection episodes occurred in 17.0% of cases. The mean age of donors was 47.3 ± 12.9 years, and 52.6% were male. Among living donors, the largest category of donors was spouses, while, among deceased donors, 31.2% were expanded-criteria donors. The mean serum creatinine concentrations of living donors were 0.78 ± 0.62 mg/dL and 1.09 ± 0.24 mg/dL at baseline and 1 year after kidney transplantation, respectively. Conclusion: The KOTRY, a systematic Korean transplant cohort, can serve as a valuable epidemiological database of Korean kidney transplants.