Objective The physiological and biochemical changes were studied during the Panax notoginseng′s fruit development.Methods Dynamic changes of fruits size,fresh and dry weight,moisture content and soluble sugar,starch and protein contents were investigated.Results The moisture content was increased first and then dropped to 73.37% at maturity.The fresh and dry weight,starch and protein contents of P.notoginseng′s fruits were increased with the fruit development.The soluble sugar content was raised first,then decreased,and increased slightly at the lateral stage of fruit development.Conclusion The P.notoginseng′s fruits are mature at 80 d after peak anthesis;the decrease of moisture content is companied with the gradual increasing of fruit fresh and dry weight during the fruit development.The storage and utilization of nutrients in P.notoginseng′s fruits are closely related with the fruit development.

The impact of the pandemic, caused by SARS-CoV-2, on the management of malignancies has posed several challenges. Gestational trophoblastic diseases, being considered highly curative, are categorized as emergent cases which need immediate intervention with a level I priority for chemotherapy for its malignant spectrum. These guidelines have been drawn up to assist in decision-making and the implementation of such are dependent on the capabilities of each institution combined with their local experience.

Objective To develop and verify a plaque method for detection of infectious titer of tick-borne encephalitis virus(TBEV)strain(PHKT strain for short)adapted to primary hamster kidney(PHK)cells.Methods PHK cells were infected with TBEV,a primary mouse brain adaption strain,and passed consecutively for 12 passages.The titer of PHKT was detected by plaque method(Monolayer BHK-21 cells were infected with PHKT of various passages at different dilution ratios,and the plaque number was calculated by neutral red staining)and challenge titration in mouse brain(Mice were challenged with PHKT of various passages at different dilution ratios through brain cavity,0.03 mL for each,observed continuously for 14 days,and calculated for the median lethal dose(LD50)by Reed-Muench method)respectively,and the correlation between the results of two methods was analyzed.The developed plaque method for the detection of TBEV titer was verified for specificity,repeatability and intermediate precision.Results The plaque titer of PHKT virus was up to8.9 lgPFU/mL;The correlation between the results of plaque method and mouse brain challenge titration method was good(r = 0.92);The specificity of plaque method for detecting infectious titer of PHKT virus was good,and the coefficients of variation(CVs)of repeatability and intermediate precision were both less than 5%.Conclusion A plaque method for detecting infectious titer of PHKT virus was developed,which may be used as an alternative method for challenge titration in mouse brain.

Objective Seek countermeasures against the debt risk in universities. Methods Taking the debts of Kunming Chenggong universities construction as an example, analyze the reasons and countermeasures for the debts of universities in Yunnan province. Results Due to the enrollment expansion policy, the higher education in Yunnan province has been transformed from the elite education into mass education. The universities in Kunming have gained unprecedented development opportunities,especially with the completion of the Kunming Chenggong University Town. However, loan funds for the new campus construction mostly came from banks. So,the huge debt stress from banks has been brought with the development of the universities. Conclusion The sustainable development of universities requires the moderate debt. The government, universities and society need to work together to control the scale of debt effectively and avoid the debt crisis.

Autophagy is a lysosomal degradation of intracellular material composition,and plays an important role in nerve cell survival and clear senescent cells and misfolded proteins.Autophagy can protect nerve cells,but also can be used as one of the ways about nerve death.Repeated seizures can cause brain damage,but the specific mechanism is not clear.Recent studies have shown that,convulsion can activate apoptosis pathway and start autophagy,which can mediate the adaptation,injury or apoptosis of neurons.Convulsion brain injury is closely related to cell autophagy.Autophagy as a new important signaling pathway can provide a new therapeutic direction of convulsion brain damage.

The internet addiction disorder is an important problem under the internet circumstance.Improving self-regulated ability is a good method to avoid the problem.The article uses the selfregulated circle theory to gain the self-regulating strategy in three phases:forethought,performance or volitional control,self-reflection.These strategies include making goal,planning strategy,self-observation and self-record,attribution.

Since from 1995, holistic nursing has over 20 years of development in China. And now as the nursing models spread to national wide, holistic nursing aim to the National Twelfth Five-year Plan. At the guidance of the National Twelfth Five-year Plan, the nursing model in China has been began to turn to holistic nursing model. But because of the influence from many factors from the implementation, the way of thinking about to carry out the nursing care using by most of the nurses in clinic is also from the illness and the medical orders. The holistic nursing philosophy and methods, for example the care program, have been not implemented well. Nurse staffing has been in the national plan. And after years of effort the situation has been improved. But it also can not fit the needs in clinic. So as the method to solve the contradiction between the staffing and the needs, the nursing schedule has began to change. The APN model and some mathematic model have been used to the innovation for the nursing schedule. The ability level of the nurses is known as the important fact on the implementation of holistic nursing. So the nurse training is in the attention. But the standardization for the training is the problem we face now. As the chief method and factor to the continual improvement for holistic nursing, quality evaluation system in our country has been improved gradually. But the scientific management methods have not well combine to the fact, and became a factor to restrict the improvement. So the necessary steps for the improvement of holistic nursing in China involve the investigation about the fact and the theory, the innovation and the standardization for the current situation, and the chinization of the theory.

Objective To study the clinical effect of nerve stimulator combined with low back piexus sciatic nerve block for the arthroscopic surgery anesthesia. Methods 86 patients who required the arthroscopic surgery from March 2010 to March 2013 in our hospital were randomly divided into observation group (43 cases) and control group (43 cases). The observation group accepted nerve stimulator combined with low back plexus sciatic nerve block under the arthroscopic surgery;the control group adopted waist hard joint anesthesia during the arthroscopic surgery. Data of hemodynamics, effects of anesthesia, anesthesias maintaining time, rehydration fluids and adverse effect in both groups were recorded and compared. Results The time for preparation and control of anesthesia operation (Tr), time for feeling completely block (T1), time for recovery (T2), time for completely blocking (T3), time for movement recovery (T4) in observation group were significantly shorter than that of the observation group (P<0.05); the first time of urination (Tu) after operation was significantly longer in control group than that in the observation group (P < 0.05); MAP in the control group were lower than that in the observation group 20 minutes after anesthesia (T20,P < 0.05); complications in the control group were more frequent than that in the observation group. Conclusion The clinical effect of nerve stimulator combined with low back plexus sciatic nerve block for the arthroscopic surgery anesthesia is more obvious , especially for patients with relatively long operating time.

Sepsis is systemic inflammatory response syndrome by infection.It always leads to sepsis shock and multiple organ dysfunction syndrome.Sepsis is the most common cause of children death,it is the hotspot and difficulty of the research field on the modem children's critical care medicine.Acute respiratory distress syndrome,is the significant cause of deterioration and death.This paper maked a review on the pathogenesis of acute respiratory distress syndrome induced by sepsis and the treatment function of Ulinastafin.

Objective:To detect the expression and significance of Dicer in gastric cell lines and gastric cancer tissue.Methods:Western-Blot and reverse transcriptase polymerase chain reaction(RT-PCR) were used to detect the expression of Dicer in normal gastric mucosa cell line GES-1,human gastric cancer cell line SGC-7901,25 surgical removed gastric cancer tissue and paired para-tumor tissue.Results:1.The expression of Dicer in human gastric cancer cell line SGC-7901 was lower than that in normal gastric mucosa cell line GES-1(P