The internet addiction disorder is an important problem under the internet circumstance.Improving self-regulated ability is a good method to avoid the problem.The article uses the selfregulated circle theory to gain the self-regulating strategy in three phases:forethought,performance or volitional control,self-reflection.These strategies include making goal,planning strategy,self-observation and self-record,attribution.

Objective To screen enterohemorrhagic Escherichia coli(EHEC) strain and declare it as a standard strain of China Medical Bacterial Species Conservation and Management Center(CMCC).On the base,to prepare strain reference and genomic DNA reference of EHECand declare them as national drug reference with independent intellectual property rights in our country.Methods According to GB4789.6-2016 National Food Safety Standards-Food Microbiology TestDiarrheagenic Escherichia coil Test,the EHEC strain was screened from 160 Escherichia coli strains from patients with diarrhea and declared as a standard strain of CMCC according to management regulations.EHEC bacterial solution and genomic DNA solution were prepared,and freeze-drying technology was used to prepare 600 strain(10~3 CFU/sample) and genomic DNA(20 ng/sample) samples respectively.20 strain and 20 genomic DNA samples were randomly selected for uniformity test.Samples storing at 25 ℃ and 37 ℃ for 1,3,5 and 7 d were taken respectively to test the transportation stability.Then the samples were tested for the short-term storage stability by storing at 4 ℃ for 7,14 and 28 d,and for the long-term storage stability by storing at 20 ℃ for 14,28 and 60 d.Three laboratories were organized for collaborative calibration.20 food products were chosen as the substrate to evaluate the application effect of strain samples.Results The one EHEC strain selected from 160 Escherichia coli strains from patients was finally declared as the CMCC(B) 43207standard strain.In the uniformity test,F_(strainsample)=0.662 0.05,and eae,stxl and stx2 of 20genomic samples were all positive.After storage at 25 and 37 ℃ for 7 d,-20 ℃ for 60 d and 4 ℃ for 28 d,the viable bacteria content of the strain samples was still 103 CFU/sample,and eae,stxl and stx2 of the genomic samples were positive.EHEC strains and genomic DNA samples selected randomly were identified as EHEC by three laboratories,the viable bacteria content was 10~3 CFU/sample,and eae, stxl and stx2 were all positive.It was detected in 20 kinds of food substrates after adding samples,but not in the background control.EHEC strain and the genomic DNA sample were included in drug standard materials in our country,numbered 80024 and 80056,respectively.Conclusion The prepared EHEC strain and genomic DNA standard materials with independent intellectual property rights can improve the timeliness of EHEC testing and make up for the gap in our country.

Anoikis is a special programmed cell death and plays important roles in both physiological regulation and pathogenesis of many diseases, including atherosclerosis, acute coronary syndrome, cardiac failure, aneurysm and tumors. This review focuses on the association of the anoikis with instability and rupture of atherosclerotic plaque.

Central nervous system(CNS) disease induces functional deficits in persons.Simultaneously,there appears axonal destruction and the formation of scar tissue,as well as cavity and physical gaps in the lesions.Olfactory ensheathing cells(OECs),acting as prime candidates for autologous transplantation,secrete neurotrophic factors for neuroprotection,angiogenesis and outgrowth of both intact and lesioned axons to different degrees,change the response status of endogenous glia after lesion,as well as remyelinate axons after a range of demyelinating insults.OECs transplantation has become an ongoing clinical therapeutic approach for human CNS disease in recent years.Thus,OECs transplantation can be used for cell-mediated repair following a variety of CNS lesions.Here,the paper reviews the current status of foundational research of OECs transplantation in treating CNS disease underlying the wide variation of results obtained in the field.

Objective To study the frequency of lymphocyte cells in hypertrophic adenoid in children with otitis media with effusion. Methods The study group consisted of 45 children suffering from hypertrophic adenoid coexisting with otitis media with effusion (HA/OME). The control group consisted of 34 children with adenoid hypertrophy(HA),but without otitis media. Adenoids excision was implemented. Cells were stained with fluorescently labeled protein-specific antibody. The lymphocyte of adenoids was determined by flow cytometry. Results Lymphocyte cells(CD3+CD4+CD69+T cells(1.29%),CD3+CD4+HLA-DR+T cells(0.61%),CD3+CD8+CD69+ T cells (1.39%),CD3+CD8+HLA-DR+ T cells (0.09%),CD19+CD69+ B cells (2.07%) and CD19+CD62L+B cells(0.21%)in HA/OME group were higher than in HA group:(CD3+CD4+CD69+T cells(1.94%), CD3+CD4+HLA-DR+T cells(0.84%),CD3+CD8+CD69+T cells(2.08%),CD3+CD8+HLA-DR+T cells(0.18%), CD19+CD69+ B cells (3.98%),CD19+CD62L+ B cells (0.46%). In addition ,there are the higher percentage lymphocyte cells(CD3+CD4+ T cells(8.97%),CD3+CD8+ T cells(4.27%),CD3+CD8+ Fas+ T cells(1.24%) and CD19+Fas+ B cells (3.23%) in HA/OME group than those in HA group:(CD3+CD4+ T cells (6.37%), CD3+CD4+CD69+ T cells (1.29%),CD3+CD4+HLA-DR+ T cells (0.61%) and CD3+CD8+ T cells(3.61%). Conclusions The local immune dysfunction resulting from hypertrophic adenoids may have a relationship with otitis media.

OBJECTIVE: Controversy over the presence of neuronal apoptosis in acute brain injuries has not been resolved due to the lack of direct evidence. In this article, the authors briefly review the current research progress of apoptosis and discuss the relationship between acute brain injuries and neuronal apoptosis.DATA SOURCES: A computer-based retrieval for relevant papers with abstracts indexed in Medline database published in English full text between January 1995 and February 2005 was conducted using the key words of "acute brain injuries" and "Neuronal apoptosis". Related articles in Chinese full text published between January 2000 and December 2004 were also searched in Chinese periodical full text database, Wanfang databases with key words of "acute traumatic brain injury, neuronal apoptosis".STUDY SELECTION: The retrieved articles were first examined to exclude review articles and repetitions, and only randomized and non-randomized controlled experiments or clinical trials on acute traumatic brain injury and neuronal apoptosis were included.DATA EXTRACTION: Altogether 136 articles directly related to acute traumatic brain injury and neuronal apoptosis were collected, among which 19 met the inclusion criteria with the other 117 removed for repetition or irrelevancy.DATA SYNTHESIS: The valid articles were reviewed to identify the factors related to neuronal apoptosis, apoptotic network theory, detection methods of apoptosis, and the relationship between acute traumatic brain injury and neuronal apoptosis as well as the possible mechanisms.CONCLUSION: Neuronal apoptosis occurs after acute traumatic brain injury, which induces intrinsic and extrinsic neuronal environment changes including increased extracellular excitatory amino acid content, massive free radical production and abnormal gene expression. These factors interact with each other and modulate neuronal apoptosis through multiple pathways.

OBJECTIVE: To probe into the progression of biochemical study on caspase-3 and its functions in disorders of center nerve system.DATA SOURCES:The relevant papers were looked up on Medline database from January 1995 to February 2005 with the retrieved words as"caspase-3", "apoptosis", "center nerve system" and "emergency diseases" in combination, limited in English version.STUDY SELECTION: By initial checking,the papers on caspase-3 in molecular biology and randomized control experiments on caspase-3 and the disorders in center nerve system were collected.DATA EXTRACTION: Totally 156 papers were collected directly related to caspase-3 and center nerve system, of which, 25 papers were in conformity with the criteria and 131 papers were exuded because of repeated contents and indirect relation with this paper.DATA SYNTHESIS: To synthesize the relevant information from the retrieved papers and sum up that the activity of caspase-3 is increased after acute cerebral injury, acute spinal injury, cerebral ischemia and cerebral hemorrhage and broadspectrum caspase-3 inhibitor, Z-VAD-fmk can reduce significantly the activity of caspase-3.CONCLUSION: Caspase-3 is the most important protease in cell apoptosis, it is the component in downstream effect of coordination of various apoptotic approaches and is the only way in protease cascade of cell apoptosis. The activated caspase-3 can split the essential and protective enzymes in cell and result in the alternations of apeptosis in morphology and biochemistry. DNA segmentation is the important biochemical property of nerve cell apoptosis at early stage in acute cerebral injury and the formation of it is necessary.

Objective To investigate the perfusion characteristics of normal pancreas and pancreatic carcinoma on multi-slice spiral CT(MSCT).Methods Perfusion CT for pancreatic cancer was performed with sixteen-slice spiral CT in 26 patients(all of them were then confirmed by pathology).Eighteen patients without pancreatic disorders served as controls.Contrast medium-enhanced dynamic CT was performed,and the data were analyzed by using commercial software to calculate tissue blood flow(BF),blood volume(BV),mean transit time(MTT),and vascular permeability-surface(PS)area product.Perfusion parameters of normal pancreas and tumors tissues were compared.Results Technical failures were experienced in 1(3.85%)subjects of pancreatic carcinoma.The mean BF,BV,MTT and PS in pancreatic carcinoma were(68.89?73.42)mL/(100 mg? min),(10.02?3.59)mL/100 mg,(8.23?3.26)s,(28.63?9.35)mL/(100 mg?min),respectively.The mean BF,BV,MTT and PS in normal pancreas tissue were(173.53?71.33)mL/(100 mg?min),(14.43?3.15)mL/100 mg,(7.15?5.45)s,(13.98?8.53)mL/(100 mg?min),respectively.The mean BF,BV and PS between pancreatic carcinoma and normal pancreas were statistically significant(BF,PS:P0.05).Conclusion CT perfusion imaging can be used to identify patients with pancreatic carcinomas,because individual differences exist in the results of CT perfusion.

AIM:To study the effects of different duration of sleep deprivation (SD) on neurogranin (Ng),protein kinase C (PKC) and Ca2+-calmodulin dependent protein kinase II (CaMK II) mRNA expressions in hippocampus and forebrain of rats. METHODS:Male Wistar rats were divided into three groups:24 h,48 h and 72 h experimental groups. Each group was divided into sleep deprivation group (SD group) and control group (C group). SD model was established by sleep deprivation box. One step methods by Trizol was used to extract hippocampus and forebrain neuronal total RNA. The changes of Ng,PKC and CaMK II mRNA expressions were detected by SYBRA green I RT-PCR. RESULTS:(1) The expression of Ng mRNA in hippocampus of SD group was significantly lower than that in C group after 24 h,48 h and 72 h SD (P