1.Observation of the early tear film changes after trabeculectomy with Keratograph 5 M
Zhong-Qi, LAI ; Wei-Na, LI ; Hui-Na, LI
International Eye Science 2015;(7):1282-1284
AlM: To observe the early tear film changes after trabeculectomy using Keratograph 5M.METHODS:Fourty-one patients (46 eyes) of glaucoma who underwent trabeculectomy were involved. First tear break up time ( FTBUT ) , average tear break up time (ATBUT), non-invasive tear meniscus height (NlTMH), lipid layer thickness and meibomian gland scores were measured at 1d preoperatively and at 1d, 1wk, 1mo postoperatively.RESULTS:At 1d, 1wk and 1mo postoperatively, FTBUT and ATBUT decreased greatly (P<0. 05), as well as lipid layer thickness (P<0. 017). NlTMH at 1d postoperatively increased significantly, but at 1wk and 1mo postoperatively decreased greatly (P<0. 05). There was no significantly difference in meibomian gland scores between preoperation and postoperation (P>0. 05). CONCLUSlON: Keratograph 5M could be used to evaluate tear film function rapidly and accurately. Trabeculectomy significantly alters tear film stability and tear secretion in the short term after operation.
2.Application of amiodarone combined with Mei TORO M in the treatment of arrhythmia
Na LAI ; Hong RAO ; Jiao YANG ; Ge EN
Chinese Journal of Biochemical Pharmaceutics 2017;37(6):228-229,232
Objective To study the clinical efficacy of amiodarone combined with metoprolol in the treatment of arrhythmia.Methods100 cases of patients with coronary heart disease arrhythmia treated in our hospital from August 2015 to September 2016 were selected as the research object in this study.They were randomly divided into the control group and the experimental group, each group had a total of 50 cases.Patients in the control group were treated with conventional arrhythmia treatment, and the experimental group was treated with amiodarone combined with metoprolol on the basis of conventional treatment.Comparative analysis of the experimental group and the control group of patients with clinical therapeutic effect index.ResultsAfter the corresponding treatment, the experimental group of 50 patients, the therapeutic effect of 18 cases were invalid, 12 cases were effective, 20 cases were markedly effective, the total effective number of cases to 32 cases, the effective rate was 64.0%.In the control group of 50 patients, 29 patients were invalid, and the effective rate was effective in all of the 8 patients.The effective rate of the treatment was effective in all of the 13 patients, with a total effective rate of 42.0%.Therefore, the effective rate of the experimental group was significantly higher than that of the control group, with statistical difference (P<0.05).The diastolic blood pressure, systolic blood pressure and heart rate in the experimental group were significantly better than those in the control group (P<0.05).There was no significant difference between the experimental group and the control group in the incidence of adverse reactions, which was not statistically significant.ConclusionAmiodarone Combined with metoprolol for treatment of arrhythmia is better, more widely used, can improve the treatment efficiency to a great extent, the treatment of high safety, with further clinical promotion and application.
3.The effect of Danhong injection on hemodynamic parameters, Cys-c, and Hcy in patients with transient cerebral ischemia
Lixin YE ; Xiaotao ZHONG ; Na LONG ; Suping LAI
International Journal of Traditional Chinese Medicine 2015;(11):984-986
Objective To detect the effect of Danhong injection on cerebral vascular hemodynamic parameters, Cys-c and Hcy in patients with transient cerebral ischemia(TIA), and analyze its clinical effect. Methods 80 TIA patients were selected. The patients were divided into a control group and a Danhong injection observation group with 40 cases each group. The control group was given conventional treatment, and the observation group was given conventional treatment and Danhong injection. The treatment course was 14 d. The hemodynamic parameters, Cys-c and Hcy expresssion were observed. Clinical effect was analyzed. Results After treatment, average blood flow speed (20.07 ± 4.28 cm/s vs. 16.17 ± 2.46 cm/s, t=5.230), average blood flow (11.14 ± 2.24 ml/s vs. 9.54 ± 1.65 ml/s, t=3.637), and cerebral vascular resistance (1 602.4 ± 98.3 kPa/s·m-1 vs. 1 738.5 ± 104.3 kPa/s·m-1, t=6.024) was significantly improved in the observation group than those in the control group (P<0.05). Cys-c (0.48 ± 0.11 mg/L vs. 0.71 ± 0.14 mg/L, t=8.170) and Hcy (17.45 ± 3.26 μmol/L vs. 23.62 ± 4.12 μmol/L, t=7.428) were significantly decreased in the observation group than those in the control group (P<0.05). The recurrence rate of TIA and cerebral infarction were 7.5% and 5% in observation group, which were significantly lower than that of 22.5% and 15% in control group (χ2=2.451, P<0.05;χ2=2.630, P<0.05).Conclusion Danhong injection can reduce the expression of Cys-c and Hcy and recurrence rate of TIA and cerebral infarction.
4.Effect of berberine on apoptosis and expression of Bax and Bcl-2 proteins in HeLa cells
Lina LAI ; Na ZHAO ; Chunhua GUO ; Xiaoyi ZHANG ; Yimin FAN
Chinese Traditional and Herbal Drugs 1994;0(02):-
Objective To investigate the effect of berberine on growth and apoptosis of human cervical cancer cell line HeLa and its possible mechanism.Methods The effect of berberine on growth of HeLa cells was studied by MTT assay.Apoptosis of HeLa cells exposed to berberine was observed by flow cytometry and DNA gel electrophoresis.The expression of Bax and Bcl-2 was studied by Western blotting analysis.Results Berberine markedly inhibited the proliferation of HeLa cells in a time-and dose-dependent manner.After incubation of HeLa cells with 20 and 40 mg/L berberine for 48 h,DNA Ladder can be observed.A typical "sub-G1 peak" was checked by flow cytometry.There was a very low rate of natural apoptosis(1.9?0.6)%,while in 5 mg/L berberine group,the apoptosis rate was(2.3?0.8)%.After exposing HeLa cells for 48 h to 20 and 40 mg/L berberine,the apoptosis rate reached(16.7?2.8)%(P
5.Investiagation of possible mechanism of eye transmission of SARS-CoV-2 based on SARS-CoV
International Eye Science 2020;20(4):729-732
SARS-CoV-2 has been spreading rapidly since its outbreak in December 2019.Understanding its epidemiological characteristics, especially cutting off transmission routes, is crucial to controlling the spread of the disease. In the study of transmission pathway, the issue of whether SARS-CoV-2 is transmitted through ocular surface tissue has also aroused concerns, but there are still no clinically confirmed cases and laboratory evidence of its infection through ocular surface tissue. New research suggests that the SARS-CoV-2 belongs to the same genus as SARS coronavirus(SARS-CoV), and that it enters cells in the same way as SARS-CoV. This paper reviews the research on SARS-CoV to investigate the possible mechanism of eye transmission of SARS-CoV-2.
6.Protein Interaction Network Construction and Biological Pathway Analysis Related to Atherosclerosis.
Quhuan LI ; Shanshan GU ; Na LI ; Zhenyang LI ; Wenlong LAI ; Yang ZENG
Journal of Biomedical Engineering 2015;32(6):1255-1260
Atherosclerosis is a complex disease characterized by lipid accumulation in the vascular wall and influenced by multiple genetic and environmental factors. To understand the mechanisms of molecular regulation related to atherosclerosis better, a protein interaction network was constructed in the present study. Genes were collected in nucleotide database and interactions were downloaded from Biomolecular Object Network Database (BOND). The interactional data were imported into the software Cytoscape to construct the interaction network, and then the degree characteristics of the network were analyzed for Hub proteins. Statistical significance pathways and diseases were figured out by inputting Hub proteins to KOBAS2. 0. The complete pathway network related to atherosclerosis was constructed. The results identified a series of key genes related to atherosclerosis, which would be the potential promising drug targets for effective prevention.
Atherosclerosis
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genetics
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Databases, Factual
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Humans
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Protein Interaction Mapping
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methods
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Protein Interaction Maps
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Software
7.Type 2 Iodothyronine Deiodinase Activity and Expressions of MBP and Synapsin I in Brain of Young Rats with Iodine Deficiency and Excess
Yi-Na SUN ; Lai-Xiang LIN ; Jia-Yu LIU ; Al ET ;
Journal of Environment and Health 2007;0(08):-
Objective To study the activity of type 2 Iodothyronine deiodinase(D2)and the expressions of myelin basic protein(MBP)and synapsinⅠin the brain tissue of young rats fed on a diet with different levels of iodine.Methods Wistar rats were fed on a diet with different doses of KIO_3 for 3 months and then mated randomly.The serum TH and the brain D2 activity were measured in 28 days old pups.The protein expressions of MBP and SynapsinⅠin their brains were determined by immunohistochemistry staining.Results Compared with normal iodine group(NI),the serum TH levels of low iodine group(LI) were lower,while those of iodine excess groups were gradually decreased with their increase of iodine intake,especially in 100-fold high iodine group(100 HI),TT_4 and FT_4 were significantly decreased(P0.05).The immunohistochemistry staining showed weakly positive reactivity of MBP in corpus callosum and stronger of synapsin I in hippocampus CA3 in LI group compared with NI. The similar alterations were also found in all iodine excess groups with their increase of iodine intake.But MBP reactivity was stronger in 100 HI rats than the LI ones.Conclusion Iodine deficiency and iodine excess can cause hypothyroidism in degrees in the young rats,more severe hypothyroid and retarded myelin sheath and synapses can be caused in iodine deficiency compared with iodine excess.
8.Molecular mechanism underlining ethanol-induced chloride currents in nasopharyngeal carcinoma cells
Na LIN ; Wanhong ZUO ; Zhouyi LAI ; Jiabao WU ; Meiyuan CHEN ; Yuan WANG ; Lixin CHEN ; Liwei WANG
Chinese Journal of Pathophysiology 2014;(7):1179-1183
AIM:To study the effects and mechanisms of ethanol on chloride channels in poorly differentiated nasopharyngeal carcinoma CNE-2Z cells.METHODS:The effect of ethanol on the cell growth was analyzed by MTT as-say.The technique of whole-cell patch-clamp was used to detect the chloride current .The characteristics of the chloride current were analyzed by using the chloride channel blockers .The siRNA technique was used to analyze the molecular basis of the ethanol-sensitive chloride channels .RESULTS: Under isotonic conditions , the background current was weak and stable.Ethanol at concentrations of 0.17~170 mmol/L activated a chloride current in a concentration-dependent manner (an inverted U-shape), with a maximum effect at the concentration of 17 mmol/L.The currents showed obviously outward rectification and were susceptible to extracellular hypertonicity and the chloride channel blocker , 5-nitro-2-(3-phenylpropyl-amino) benzoic acid ( NPPB) .ClC-3 siRNA obviously decreased the currents activated by ethanol .CONCLUSION:Ex-tracellular ethanol induces chloride currents through activating the ClC-3 chloride channels .
9.Kinetic adsorption of Herba Artemisiae scopariae extract by macroporous resin
Fengyun WANG ; Bin JIANG ; Yuaner ZENG ; Na CHEN ; Xiaoping LAI ; Ju ZHANG
Chinese Traditional Patent Medicine 1992;0(04):-
AIM: To study the kinetic adsorption process in the use of the extraction of Herba Artemisiae scopariae. METHODS: In combination with caffeotannic acid and the absorbance, HPLC fingerprint of extract of Herba Artemisae scopariae was adopted as marker, to investigate the kinesis adsorb and elution process, the to determine the max quantity of physic liquor and the type and quantity of elution solvent. RESULTS: The max adsorption quantity of caffeotannic acid that could adsorb was 20.9 mg/g dry resin, and the elution solvent determined was five-fold column volume of 80% alcohol; The repeated experiment indicated that this process decressed to 2.5% yield of extract and meanwhile it retained the whole components of the HPLC fingerprint effectly. CONCLUSION: The kinetic adsorption can enrich the active components in Herba Artemisiae scopariae effectively.
10.Regulation of expression of pituitary tumor transforming gene 1 (PTTG1) by androgen in prostate cancer
Dianqi XIN ; Xuhui ZHU ; Yongqing LAI ; Ran YOU ; Yanqun NA ; Yinglu GUO ; Zebi MAO
Journal of Peking University(Health Sciences) 2003;0(06):-
Objective:To identify the androgen-responsive genes in prostate and screen the molecular targets for further studying human prostate cancer. Methods:The potential androgen-responsive gene pituitary tumor transforming gene 1 (PTTG1) was selected which had been previously screened by cDNA microarray in rat prostate and its mRNA level was detected by Northern blot in the castrated rat prostate with and without replacement of Mibolerone. Immunohistochemistry was performed to determine the expression and location of PTTG1 in human prostate tissues. Then human androgen-dependent prostate cancer cells LNCaP were used as a model to study the regulation of PTTG1 by Mibolerone. Results: PTTG1 mRNA was hardly detectable in the prostate of 7-day castrated rats, while it was up-regulated dramatically in the prostate of 7-day castrated rats treated with Mibolerone for 2 days. It was showed that high expression of PTTG1 was localized to the epithelial cells of human prostate cancer but not to the stromal cells with Immunohistochemistry. Northern blot analysis indicated that LNCaP cells treated with 0.1 nmol/L Mibolerone for 2 days led to the high PTTG1 mRNA expression. The basic expression of PTTG1 in human androgen-independent prostate cancer cell lines PC3 or DU145 was even higher than that in the human androgen-dependent prostate cancer cells LNCaP treated with Mibolerone. Conclusion: Androgen can up-regulate the PTTG1 expression in castrated rat prostate and human prostate cancer cell LNCaP. It suggests that PTTG1 is potential to play an important role in human prostate cancer progression.