The study of quality control of Chinese medicine has always been the hot and the difficulty spot of the development of traditional Chinese medicine (TCM), which is also one of the key problems restricting the modernization and internationalization of Chinese medicine. Multivariate statistical analysis is an analytical method which is suitable for the analysis of characteristics of TCM. It has been used widely in the study of quality control of TCM. Multivariate Statistical analysis was used for multivariate indicators and variables that appeared in the study of quality control and had certain correlation between each other, to find out the hidden law or the relationship between the data can be found,.which could apply to serve the decision-making and realize the effective quality evaluation of TCM. In this paper, the application of multivariate statistical analysis in the quality control of Chinese medicine was summarized, which could provided the basis for its further study.
Medicine, Chinese Traditional ; standards ; Multivariate Analysis ; Quality Control

Adolescent ; Child ; Child, Preschool ; Electroencephalography ; Female ; Humans ; Infant ; Retrospective Studies ; Rett Syndrome ; pathology ; physiopathology

Animals ; Carnitine ; pharmacology ; H(+)-K(+)-Exchanging ATPase ; metabolism ; Mitochondria, Muscle ; enzymology ; Muscle, Skeletal ; drug effects ; Physical Conditioning, Animal ; Rats

?AIM:To investigate the measurement of central anterior chamber depth ( ACD ) in patients with acute primary angle - closure glaucoma ( APACG ) with A - scan ultrasound, Pentacam and ultrasonic biological microscope ( UBM) .?METHODS: Thirty-five patients (35 eyes) with APACG were selected, of whom central ACD were measured with A-scan ultrasound, Pentacam and UBM.?RESULTS: The measurement values of ACD with A-scan ultrasound, UBM and Pentacam were 1. 5633±0. 2089, 1. 5783 ± 0. 2067, 1. 6275 ± 0. 2296mm, which was equal variance tested by the homogeneity of variance, and was significant different by multiple comparision (F=4. 074, P=0. 026). The difference of ACD between the two groups of A-scan ultrasound and UBM, A-scan ultrasound and Pentacam, UBM and Pentacam were statistically significant ( P = 0. 032, 0. 023, 0. 012 ). Altman- Bland analysis showed that the three methods were not consistent with each other.?CONCLUSION: The ACD value of the APACG with the three methods is the largest using Pentacam, followed by UBM and A - scan ultrasound. In clinical the three methods with different advantages can complement each other, but cannot be replaced. In order to obtain more accurate results, we should combine the advantage and make comprehensive analysis.

AIM:The polysaccharides of Hedyotis difusa Willd of different sources were extracted and its content and composition of the polysaccharide was analyzed. METHODS: The microwave was employed for the aqueous extract of Hedyotis difusa Willd and the content of total sugar was determined by phenol-sulfur test,the monosaccharides composition was determined by HPLC with pre-column derivatization. RESULTS: The polysaccharide obtained from the samples of Guangdong Province had the highest content.The analytic data showed that the composition of monosaccharides in hydrolytic polysaccharides of Hedyotis difusa Willd were mannose,glucose,galactose,arabinose and rhamnose,the average content of polysaccharides was 9.82%. CONCLUSION: The HPLC method with pre-column derivatization is appropriate for the analysis of monosaccharide composition of Hedyotis difusa Willd polysaccharides and the method is simple,quick and accurate.

Objective: This study aimed to clone and characterize the oxiranedicarboxylate hydrolase (ORCH) from Labrys sp. WH-1. Methods: Purification by column chromatography, characterization of enzymatic properties, gene cloning by protein terminal sequencing and polymerase chain reaction (PCR), and sequence analysis by secondary structure prediction and multiple sequence alignment were performed. Results: The ORCH from Labrys sp. WH-1 was purified 26-fold with a yield of 12.7%. It is a monomer with an isoelectric point (pl) of 8.57 and molecular mass of 30.2 kDa. It was stable up to 55 °C with temperature at which the activity of the enzyme decreased by 50% in 15 min (T5015) of 61 °C and the half-life at 50 °C (t1/2, 50 °c) of 51 min and was also stable from pH 4 to 10, with maximum activity at 55 °C and pH 8.5. It is a metal-independent enzyme and strongly inhibited by Cu2+, Ag+, and anionic surfactants. Its kinetic parameters (Km, kcat, and kcat/Km) were 18.7 mmol/L, 222.3 s−1, and 11.9 mmol/(L·s), respectively. The ORCH gene, which contained an open reading frame (ORF) of 825 bp encoding 274 amino acid residues, was overexpressed in Escherichia coli and the enzyme activity was 33 times higher than that of the wild strain. Conclusions: The catalytic efficiency and thermal stability of the ORCH from Labrys sp. WH-1 were the best among the reported ORCHs, and it provides an alternative catalyst for preparation of l(+)-2,3-dihydrobutanedioic acid.

This study was aimed to isolate and characterize of chemical constituents in a traditional Mongolian medicine HERBA CLEMATIS. Normal and reverse phase coloumn chromatography, gel filtration chromatography sephadex LH-20, and preparative HPLC were used for isolation and purification compounds from the water extraction of the arial part ofC. aethusaefoliaTurcz. The planar structures and spatial configurations of isolated compounds were identified by high resolution MS, 1D and 2D NMR, and other spectrographic methods. The chemical research on the Mongolian medicine results 6 compounds, dihydrodehydrodiconiferyl alcohol (1), syringaresinol (2), pinoresinol (3), epi-pinoresinol (4), lirioresinol B dimethyl ether (5) and loliolide (6). All the compounds were isolated from this plant for the first time.

Objective To evaluate the effect of ultrasound-guided mid-humeral block on the motor function of upper extremities of the patients undergoing day-case surgery in a department of hand surgery. Methods Thirty patients,weighing 50-75kg,aged 18-64 yr,of American Society of Anesthesiologists physical statusⅠ-Ⅲ,scheduled for elective hand,wrist or forearm surgery,were divided into group Ⅰ(n=15)and group Ⅱ(n=15)using a random number table. Ultrasound-guided mid-humeral block was performed in group Ⅰ,and ultrasound-guided supraclavicular brachial plexus block was performed in group Ⅱ,both with 0.375% ropivacaine 25ml. The onset time of sensory block,recovery time of sensory function,recovery time of motor function of shoulder and elbow joints,allowable hospital discharge time and patient′s satisfaction were recorded. Results Compared with group Ⅱ,the onset time of sensory block,recovery time of motor function of shoulder and elbow joints and allowable hospital discharge time were significantly shortened,and the degree of patient′s satisfaction was increased in group Ⅰ(P<0.01).There were no significant differences in the recovery time of sensory function between the two groups(P＞0.01).Conclusion Ultrasound-guided mid-humeral block has shorter onset time and less influence on the motor function of upper extremities than ultrasound-guided supraclavicular brachial plexus block in the patients undergoing day-case surgery in a department of hand surgery.

Objective To explore the effect of manidipine hydrochloride on kidney function in patients with hypertension. Methods One hundred and thirty -three hypertensive patients with renal dysfunction were randomly divided into treatment group ( n=73 ) and control group (n=60).Treatment group was treated with manidipine 10 mg? d-1,qd, and control group was treatment with amlodipine 5 mg? d-1 for 2 weeks. If the diastolic pressure≥90 mmHg after treatment, the manidipine in-creased to 20 mg? d-1 and amlodipine increased to 10 mg? d-1 .The trial lasts 13 weeks totally.The blood puressure, heart rate, blood creatinine and 24 h urinary protein of the two groups were observed. Results The blood pressure was significantly decreased in both groups (P<0.05).Heart rate was significantly reduced in treatment group ( P<0.05) , the average blood creatinine levels dropped to normal range, 24 h urinary protein didn′t decrease to normal, but significantly declined( P<0.05) , and the creatinine clearance rate had no significant difference.The creatinine and creatinine clearance rate were significantly difference compared with those before treatment in control group ( P<0.05) , the average of 24 h urinary protein excretion significantly increased compared with before treatment. Conclusion Manidipine hydrochloride presents a useful antihypertensive and renoprotective effect on mild -to -moderate essential hypertension with kidney dysfunction patients, with no heart inhibition.

Chemical and Drug Induced Liver Injury ; epidemiology ; Humans ; Incidence