Objective:To examine the social support mechanism of pet ownership and pet attachment.Method:A total of 321 pet owners and 418 non pet owners above 40 years in Beijing were measured with questionnaires.Results: (1) Pet ownership showed significant correlations with physical health and life satisfaction.Compared with non pet owners,pet owners had better physical health and life satisfaction (β=0.074;P<0.05,β=0.110;P<0.01).(2)Pet ownership could change the effect of recent life events on the physical health but not on life satisfaction (Z=2.59,P<0.01).(3) Pet attachment showed significant correlations with life satisfaction.The pet owners with more pet attachment had higher life satisfaction.Conclusions:Pet ownership has significant main effect of social support as well as buffering effect of social support.They appear as the significant moderating effects on recent life events and physical health.Pet attachment has significant main effect of social support,and few buffering effects of social support.

Objective: To investigate the relationship between attachment to pets and psychosecial develop-ment of children. Methods: Totally 147 children pet owners from grade 3 to grade 5 were selected from 11 primary schools in Beijing. They were asked to complete the Lexington Attachment to Pets Scale, Self Description Question-naire, Children's Loneliness Scale, and 2 dimensions of Children Social-life Adjustment Scale. Results: Attach-ment to pets was positively correlated with self-concept, pro-social orientation and living ability of daily life (r=0.47, 0.42, 0.34, Ps < 0.001), and negatively correlated with loneliness (r=-0.28, P <0.01) . Even the variables including study performance, interrelationship and socioeconomic status of family were taken as covari-ares, the higher attach group was better than lower attach group in self-concept, pro-social orientation, living abili-ty of daily life [(163.81±17.81)vs.(138.36±27.71), (46.19±4.25)vs.(40.64±6.93), (39.05±4.17)vs.(33.77±6.51); Ps<0.001] and loneliness [(28.53±11.08)vs.(34.79±10.96); P <0.01].Conclusion: There is apparent relationship between pet attachment and psychosocial development in children.

Objective To investigate the effect of penehyclidine (PHCD) on Toll-like receptor 4 (TLR4)mRNA and Toll-like receptor 2 (TLR2) mRNA expression in the lung tissue in rats with acute lung injury induced by lipopolysaccharide (LPS) .Methods Sixty healthy SD rats of both sexes weighing 200-220 g were randomly divided into 5 groups ( n = 12 each) :control group (group C) , LPS group and P1-3 groups. Acute lung injury was induced by intraperitoneal (IP) LPS 8 mg/kg in LPS and P1-3 groups. PHCD 0.3, 1.0 and 3.0 mg/kg were given IP after LPS administration in P1-3 groups. The animals were anesthetized at 6 h after IP LPS. Blood samples were collected for determination of serum TNF-α and IL-6 concentrations ( by ELISA) and then sacrificed, the lungs were immediately removed for determination of TLR4 mRNA and TLR2 mRNA expression (by RT-PCR), and microscopic examination. Results LPS significantly increased TLR4 mRNA and TLR2 mRNA expression in the lung tissue and serum TNF-α and IL-6 concentrations. PHCD 1.0 or 3.0 mg/kg significantly inhibited LPS-induced increase in TLR4 mRNA and TLR2 mRNA expression in the lung tissue and serum TNF-α and ILr6 concentrations.The lung histopathologic damage was significantly ameliorated in P2 and P3 groups as compared with group LPS.Conclusion PHCD can protect the lungs against LPS-induced acute lung injury through inhibiting TLR4 mRNA and TLR2 mRNA expression in the lung tissue and reducing the inflammatory response.

Objective To investigate the cytidylyl phosphate guanosine(CpG) islands methylation status of E-cadherin (E-cad) promoter region in human ovarian carcinoma cell lines (ES-2,3 AO, SKOV3 ), and the effect of 5-azacytidine-2 '-deoxycytidines (5-Aza-CdR ) on the cell proliferative ability, invasion and the expression of E-cad protein. Methods Methylation specific PCR(MSP) was used to detect CpG islands methylation status of E-cad promoter region in ES-2,3AO and SKOV3 cell lines. After treated with different concentrations of 5-Aza-CdR, morphological changes of cell lines were observed under microscope. The proliferative ability was evaluated by methyl thiazolyl tetrazolium(MTT) assay. E-cad protein expression was detected by western-blot and cellular invasion was investigated by 24-well matrigel invasion chambers. Results Hypermethylatian status of CpG islands of E-cad promoter region was observed in ES-2 and SKOV3 cell lines, but not in 3AO cell lines. After treated with 5-Aza-CdR (0.1,1.0,10.0 μmol/L), ES-2 and SKOV3 cell lines displayed morphological evidence of differentiation. 5-Aza-CdR was found to decrease proliferation as evidenced by cell growth curve , to increase the level of E-cad protein expression (P ＜ 0.01 ), and effectively inhibit the ability of cell invasion(P ＜0.01 ). Conclusions CpG hypermethylation is an important mechanism of E-cad gene inactivation in ES-2 and SKOV3 cell lines. 5-Aza-CdR be found to inhibit proliferation and invasion, and increase the expression of E-cad probably by the inhibition of hypermethylation.

The pathogenesis of multiple sclerosis ( MS) involves alterations to multiple pathways and processes, which represent a significant challenge for developing more-effective therapies.In MS, abnormalities have been identified in several cytokine-signaling pathways, as well as those of other immune receptors.Among the downstream molecules implicated are Jak/Stat, NF-κb, ERK1/2, p38 or Jun/Fos, current MS drugs target some of these pathways.This article will with the aid of the latest research results of systems biology approaches that study pathway dysregulation in the process of MS development, targeting these relevant MS-signaling pathways, offers the opportunity to accelerate the development of novel individual or combination therapies for the future of new drug research.

Objective To investigate the relationship between values of blood calcium, serum urea nitrogen (BUN), D-dimer, C-reactive protein (CRP), fibrinogen and amylase with the severity of the disease in patients with acute pancreatitis (AP). Methods There were 70 patients with mild AP (MAP group), 18 patients with moderate AP (MSAP group), 26 pa?tients with severe acute pancreatitis (SAP group) in 114 AP patients. The laboratory indexes were compared between these groups. The correlation between indexes and the acute physiology and chronic health evaluation systemⅡ (APACHE Ⅱ) score was analysed. The diagnostic sensitivity of SAP using CRP, D-dimer and fibrinogen was analysed by ROC curves. Re?sults Compared with MAP group, values of BUN, CRP, D-dimer,fibrinogen and APACHEⅡscore were significantly increased in SAP group (P<0.05), but serum calcium level was significantly decreased (P<0.05). The APACHEⅡscore were significantly higher in SAP group than that of MSAP group (P<0.05). There were no significant differences in level of amylases between three groups. There was a positive correlation between APACHEⅡscore, CRP, D-dimer and fibrinogen (r=0.407, 0.404 and 0.245, P<0.05). There was a negative correlation between APACHEⅡscore and serum calcium level (r=-0.333, P<0.05). The area under the ROC curve showed a maximum CRP curve for diagnosing SAP 0.752 (95%CI=0.644-0.860). The cut-off value was 74.45 mg/L. The sensitivity was 86.4%. And the specificity was 68.2%. Conclusion Combining with monitoring BUN, blood coagulation index, CRP, serum calcium level and other laboratory parameters was useful to overall evaluate AP patients and improve the prognosis.

Objective To study more significant detective combination by comparing three methods of antinuclear antibody,extractable nuclear antigen and antinuclear antibodies 3.Methods Three methods were adopted as follows:detecting antinuclear antibody with HEP-2 and prime hepar as antigen media,determining extractable nuclear antigen by EUROASSAY including nRNP/Sm、Sm、SSA、SSB、Scl-70 and Jo-1,using crithidia iuciliae as antigen,to detect ds-DNA antibody with indirect immunofluorescence assay,and to study 15 autoantibodies by EUROLINE.Results To compare positive rates of three dilution(1:10,1:80,1:100)in ANA detection,there existed significant difference(P<0.01)between two dilution of 1:10 and 1:80 only,.In the detection of multiple nuclear types(cytoplasmic pattern,centromere pattern and homegeneous pattern etc),the positive rate of antinucleatr antibodies 3 was higer than that of ENA.IIF was prior to the EUROLINE in the detection of ds-DNA.Conclusion During detecting ANA with IIF,the dilution of 1:80 has advantages in raising positive rate,and ANA3 of 15 antigens provides facilities for clinical diagnosis.

Along with the progressing of micro-electronics technology and the improving of FPGA's performance, it's more and more common to process digital signals using FPGA. In most cases, FPGA is used to do the preprocessing or postprocessing besides processor in order to get a higher processing speed. This article introduces how to construct the signal processing unit of Doppler measurement system with FPGA and universal digital signal processor.

Yes-associated protein (YAP) is a multifunctional protein that can interact with different transcription factors to acti-vate gene expression. YAP, as the key transcription protein of Hippo pathway, has made important contribution to the control of the or-gan size, stem cell biology, and tissue-specific stem cell self-renewal. In the Hippo pathway, MST1/2 kinases, together with the adaptor protein SAV, phosphorylate LATS1/2 kinases. YAP is phosphorylated and inhibited by the activated LATS1/2, a key component of the Hippo tumor suppressor pathway. In recent years, numerous studies have shown that the high expression of YAP in the embryonic stem cells, neural stem cells, and hematopoietic stem cells can maintain the cancer stem cell;and YAP has become the marker of cancer stem cells. Meanwhile, more and more studies indicate that the disorder of Hippo-YAP signaling pathway may be associated with cancer stem cells. In this review, we mainly discuss the role of the Hippo pathway in the stem cell biology and its potential implications in tis-sue homeostasis and cancer.

In order to improve the diagnosis and treatment of severe hemolytic uremic syndrome (SHUS),we summarized the rescuing experiences in 3 children with SHUS.Therefore,close observation of gastro intestinal symptoms,collaborated with repeated examinations of the blood,renal function,urinary output,blood pressure,and manifestations of the central nervous system could help predict to certain extent the occurrence of further hemolysis and renal failure in these patients.Energetic treatment of malignant hypertension and relieving the symptoms of center nervous system could successfully improve renal functions.If urinary output decreased quickly,creatinine (Cr) and blood urea nitrogen (BUN) increased,or severe infection occurred in SHUS patients,hemodialysis should be undertaken as early as possible to prevent caute renal failure and multiple organ failure syndrome (MOFS).Therefore,the treatment of SHUS in children has its peculiarity and complexity.