Objective The physiological and biochemical changes were studied during the Panax notoginseng′s fruit development.Methods Dynamic changes of fruits size,fresh and dry weight,moisture content and soluble sugar,starch and protein contents were investigated.Results The moisture content was increased first and then dropped to 73.37% at maturity.The fresh and dry weight,starch and protein contents of P.notoginseng′s fruits were increased with the fruit development.The soluble sugar content was raised first,then decreased,and increased slightly at the lateral stage of fruit development.Conclusion The P.notoginseng′s fruits are mature at 80 d after peak anthesis;the decrease of moisture content is companied with the gradual increasing of fruit fresh and dry weight during the fruit development.The storage and utilization of nutrients in P.notoginseng′s fruits are closely related with the fruit development.

The impact of the pandemic, caused by SARS-CoV-2, on the management of malignancies has posed several challenges. Gestational trophoblastic diseases, being considered highly curative, are categorized as emergent cases which need immediate intervention with a level I priority for chemotherapy for its malignant spectrum. These guidelines have been drawn up to assist in decision-making and the implementation of such are dependent on the capabilities of each institution combined with their local experience.

Objective To develop and verify a plaque method for detection of infectious titer of tick-borne encephalitis virus(TBEV)strain(PHKT strain for short)adapted to primary hamster kidney(PHK)cells.Methods PHK cells were infected with TBEV,a primary mouse brain adaption strain,and passed consecutively for 12 passages.The titer of PHKT was detected by plaque method(Monolayer BHK-21 cells were infected with PHKT of various passages at different dilution ratios,and the plaque number was calculated by neutral red staining)and challenge titration in mouse brain(Mice were challenged with PHKT of various passages at different dilution ratios through brain cavity,0.03 mL for each,observed continuously for 14 days,and calculated for the median lethal dose(LD50)by Reed-Muench method)respectively,and the correlation between the results of two methods was analyzed.The developed plaque method for the detection of TBEV titer was verified for specificity,repeatability and intermediate precision.Results The plaque titer of PHKT virus was up to8.9 lgPFU/mL;The correlation between the results of plaque method and mouse brain challenge titration method was good(r = 0.92);The specificity of plaque method for detecting infectious titer of PHKT virus was good,and the coefficients of variation(CVs)of repeatability and intermediate precision were both less than 5%.Conclusion A plaque method for detecting infectious titer of PHKT virus was developed,which may be used as an alternative method for challenge titration in mouse brain.

Alzheimer Disease ; therapy ; Amyloid beta-Peptides ; metabolism ; Animals ; Humans ; Medicine, Chinese Traditional

Acute Disease ; Bacterial Translocation ; C-Reactive Protein ; analysis ; Calcitonin ; blood ; Child ; Humans ; Pancreatitis ; diagnosis ; etiology ; therapy ; Protein Precursors ; blood

Books ; Medicine, Chinese Traditional ; Translations

Objective: To explore the safety of using diagnostic hysteroscopy in patients with clinical stage one endometrial cancer,whether diagnostic hysteroscopy can increases the risk of positive peritoneal cytology in such patients and affects their post-operative prognosis.Methods: Medical records of 52 patients with clinical stage one endometrial cancer diagnosed by pre-operative hysteroscopy in our hospital were reviewed in this retrospective study,informations about cytological test of abdominal peritoneal fluid,type of operation,post-operative surgical stage and follow-up were collected.Results: The mean interval between hysteroscopy and laparotomy was 36 days(5~82d),2/52(3.8%) present positive peritoneal cytology,the mean survival time was 81 months(9~130m),the 5-year survival rate was 91.5%.Conclusion: Diagnostic hysteroscopy had not increase the risk of positive peritoneal cytology,also had no adverse effect on prognosis in clinical stage one endometrial cancer patients.

0.05),and adverse drug reaction was none in the trial group vs.7.5% of the control group.CONCLUSIONS: Sodium Fusidate is as effective as vancomycin but has fewer side effects for orthopedic patients infected with MRSA.

OBJECTIVE:To study the general pattern and characteristics of the adverse drug reactions (ADR) induced by mannitol.METHODS:The literature related to the ADR induced by mannitol reported during 1999～2008 retrieved from CNKI was analyzed statistically.RESULTS:A total of 135 mannitol-induced ADR cases were collected,of which,males showed higher proportion than females,and the ADRs chiefly manifested as acute renal failure(30 cases,22.22%),followed by allergic reactions(17 cases,12.59%) and shock(17 cases,12.59%).CONCLUSION:Clinicians should tighten monitoring on the use of mannitol to reduce the occurrence of mannitol-induced ADR.

Alkyl and Aryl Transferases ; genetics ; Child ; DNA, Mitochondrial ; genetics ; Fibroblasts ; metabolism ; Glomerulosclerosis, Focal Segmental ; diagnosis ; drug therapy ; genetics ; Humans ; Kidney Diseases ; diagnosis ; drug therapy ; genetics ; Mitochondrial Diseases ; diagnosis ; drug therapy ; genetics ; Mutation ; Nephrotic Syndrome ; diagnosis ; drug therapy ; genetics ; Protein Kinases ; genetics ; Ubiquinone ; analogs & derivatives ; biosynthesis ; deficiency ; therapeutic use