Animals ; Biomarkers ; analysis ; metabolism ; Carcinoma, Hepatocellular ; diagnosis ; metabolism ; Chromatography, High Pressure Liquid ; methods ; Discriminant Analysis ; Fatty Liver ; diagnosis ; metabolism ; Humans ; Liver Failure ; diagnosis ; metabolism ; Liver Neoplasms ; diagnosis ; metabolism ; Mass Spectrometry ; methods ; Metabolomics

BACKGROUND: There are several ways for treating the trigeminal neuralgia, such as medication, block therapy and surgery, etc. But few reports throughout the world described stereotactic radiotherapy(SRT) for it, which is efficient, non-invasive and painless.OBJECTIVE: To investigate the improvement of SRT for trigeminal neuralgia.DESIGN: Case analysis based on patients.SETTING: X-knife Treatment Center, First Affiliated Hospital, Henan University of Science and Technology.PARTICIPANTS: Totally 30 patients with trigeminal neuralgia were selected from X-knife Treatment Center, the First Affiliated Hospital of Henan Science and Technology University between March 1999 and March 2002. All patients were diagnosed with CT or MRI, and no other diseases were found. Patients without obvious effect from a long-term medicine treatment or surgery were selected as the investigative subjects.METHODS: Thirty patients with trigeminal neuralgia wearing face cover were treated with non-coplanarity fractional stereotactic radiotherapy. The patients received 2 target regions and peripheral does of 60 - 75 Gy. 5 mm collimator and isodose curves referred to ranged from 85% - 90%.MAIN OUTCOME MEASURES: Results of therapeutic assessment and follow up.RESULTS: The follow up period ranged from 1 to 22 months. Among 30patients, 6 patients' symptoms were lightened or eased up over 12-24hours, 20 patients received a marked effect over 1 - 3 months, 4 patients'treatment effects were not obvious. The effect rate reached 87%. A patient occurred numbness in sick face, and a patient showed no obvious effect in the first treatment, but the efficacy became evident in the second treatment.CONCLUSION: It is efficient in the treatment of trigeminal neuralgia with stereotaetic radiotherapy and there is no special side effect.

Renal interstitial fibrosis is the common pathway for a variety of chronic renal disease to final stage renal failure,the main feature of it is the large accumulation of extra cellular matrix (ECM).Myofibroblasts is the major effector cells which lead to the synthesis of ECM,an important source of myofibroblasts is epithelial-mesenchymal transition(EMT).Wnt/β-catenin signaling pathway take part in the regulation of EMT,which make it become a potential site of anti-fibrosis therapy.

Objective To explore the effect of hyperbaric oxygen (HBO) on the apoptosis resulted from hypoxic-ischemic brain damage (HIBD) and potential relation to caspase-3 expression.Methods Rats were divided into HIBD、HBO、sham operation group.To observe the morphologic change of brain tissues treated by HBO and compared with that of the hypoxia-ischemia rat model, then check the dynastic change of caspase-3 expression by immunohistochemistry at different time point. Results The expression of caspase-3 in the hippocampus and cortex was higher than that of sham group at 18, 24, 48 and 96 h time point(P

Humans ; Ankle ; Acupuncture Therapy ; Stroke

Adult ; Female ; Humans ; Maxillofacial Abnormalities ; Nose ; abnormalities

Objective To investigate dry chemical analysis of urine,automated quantitative anal-ysis of urine formed elements and urine living cells staining microscope extination combination of the three urine red blood cells for a variety of detection methods in the comprehensive analysis of renal dis-ease in the clinical application. Methods Gemany Miditron Junior Ⅱ of urine analyzer for chemical a-nalysis of urine. UF-1 00 automatic urine visible component analysis(referred to: UF-1 00)living cells (SM)staining, The difference in the imaging system under the microscope, in the urine of red blood cells to identify patterns observed. Results Urine dry chemical analysis,automated quantitative analy-sis of urine fomed elements and ,urlne staineg cells microscope examination of the three organic combi-nation of a variety of detection methods for urine analysis, Application of this paper, Detection of a va-riety of red blood cells urine analysis-urine flow chart of sources of identification laboratory, Improve the analysis of the urine test quality, efficiency and laboratory dinosis, made up of these expenmental methods of the deficiencies. Conclusion Kidney disease is extremely valuable to provide obj ective indi-cators, is in clinical methods.

Objective To analysis of the thyroid hormone test results of Abbott Architect ci16200 automatic biochemical-im-mune analyzer (Abbott system) and Roche Cobas e601 automated immunoassay analyzer (Roche system) .Methods The thyroid hormone(including T4 ,FT4 ,T3 ,FT3 ,TSH ) levels of 93 cases of serum samples were respectively detected by Abbott system (CMIA) and Roche system (ECLIA) ,and the results were analyzed .Results The thyroid hormone test results of the two systems had a good correlation ,and the coefficients of T4 ,FT4 ,T3 ,FT3 ,and TSH were 0 .960 ,0 .962 ,0 .976 ,0 .900 ,and 0 .999(P<0 .01) . However ,there were significant difference of the detection results of the two systems(P<0 .05) .Conclusion The thyroid hormone test results of Abbott system and Roche system are not comparable .

OBJECTIVE:To study the effects of podophyllotoxin derivative QW-83 on human cervical cancer HeLa cell apopto-sis and its mechanism. METHODS:After treated with 0(negative control),0.01,0.1,1 and 10 μmol/L QW-83 and positive drug etoposide(VP-16)for 48 h,proliferation inhibition rate and IC50 of HeLa cell were determined by MTT assay. The morphological changes of HeLa cell were observed by Hochest 33342 staining after treated with QW-83 [0(negative control),2.5,5,10μmol/L] for 48 h;flow cytometry was used to detect apoptosis rate;semi quantitative RT-PCR was adopted to detect the expression of apop-tosis related gene P53,Bax,Casepase-3,Casepase-8,Casepase-9 and Bcl-2 mRNA. RESULTS:Compared with negative control, 1,10 μmol/L VP-16 and QW-83 had obvious proliferation inhibition effect on HeLa cells (P<0.05 or P<0.01),and IC50 were (5.11±0.43)μmol/L and(4.96±0.54)μmol/L. Hochest 33342 staining results showed QW-83 could obviously induce cells apopto-sis and nuclear pyknosis. Flow cytometry showed QW-83 could increase apoptosis rate in concentration-dependent manner,being 16.89%-62.56%. RT-PCR showed mRNA expression of P53,Bax,Caspase-3,Casepase-8 and Casepase-9,Bcl-2/Bax increased, while mRNA expression of Bcl-2 decreased after treated with QW-83(P<0.05). CONCLUSIONS:Podophyllotoxin derivative QW-83 can induce HeLa cell apoptosis,and its mechanism may be associated with regulate mRNA expression of apoptosis related gene.

Acupuncture Points ; Adult ; Female ; Humans ; Male ; Meridians ; Radiculopathy ; diagnosis ; therapy ; Spondylosis ; diagnosis ; therapy