1.Mast cell tryptase and asthma
Chinese Journal of Pathophysiology 2000;0(08):-
As an important mediator of allergic inflammation, mast cell tryptase is involved in the induction of hypersensitivity, infiltration of inflammatory cells and tissue remodeling in respiratory tract. The effects of tryptase inhibitors on the actions of tryptase show further the potential of tryptase in the pathogenesis of asthma and its inhibitors in the treatment of asthma.
2.Transforming growth factor ?1 induces apoptosis in mouse odontoblast cell line MDPC-23
Wenxi HE ; Zhongying NIU ; Shouliang ZHAO
Journal of Practical Stomatology 1995;0(04):-
Objective:To investigate if transforming growth factor-?1 (TGF-?1) induces apoptosis in odontoblast cell line MDPC-23. Methods:MDPC-23 cells were treated with TGF-?1 at 0.5,2.5 or 10 ng/ml for 48 h,apoptosis of MDPC-23 cells was detected by annexin V and propidium iodide (PI) staining, cell death detection ELISA and DNA electrophoretic analysis. Results: Consistent results were obtained from three different methods.TGF-?1 at 0.5-10 ng/ml induced apoptosis of MDPC-23 cells in a dose-dependent manner. Conclusion:TGF-?1 may paly a role in apoptosis of odontoblasts.
3.Molecular mechanisms of transformation from actinic keratosis to squamous cell carcinoma
Ruixian NIU ; Dan XU ; Li HE
Chinese Journal of Dermatology 2016;49(2):144-146
Actinic keratosis (AK) is a precursor to cutaneous squamous cell carcinoma (SCC),and molecular mechanisms of transformation from AK to SCC have been a research hotspot.This review focuses on four aspects of the molecular mechanisms,including chromosomal variation,gene mutations,signaling pathways and other factors,summarizes advances in selective mutations in chromosomes 3p,9p,9q,13q,17p and 17q,heterozygous deletion mutations in chromosome 18q,mutations of p53,p16 and Ras genes,abnormality of the transforming growth factor β1 signaling pathway and Fas/FasL signaling pathway,and aberrant expression of matrix metalloproteinases in the transformation from AK to SCC,which will provide a reference for further researches.
4.Correlation between cerebral microbleeds and long term aspirin administration in non-hypertensive patients
Lihong GE ; Guangming NIU ; Jinlong HE
Journal of Practical Radiology 2017;33(1):5-7,19
Objective To investigate the correlation of long term aspirin treatment and cerebral microbleeds(CMBs)incidence in non-hypertensive patients.Methods 56 non-hypertensive patients (the average age of 64.88±6.99)with long term aspirin adminis-tration (100 mg/d)were enrolled in the study from 2005 to 2010 in our hospital,with follow up to compare CMBs 5-10 years later. All patients underwent T2 WI,T1 WI,diffusion-weighted imaging (DWI)and susceptibility weighted imaging (SWI).The CMBs lesions were defined by senior radiologists.Patients’age,gender,total cholesterol level,aspirin,CMBs and CMBs location were taken into account in data analysis.Results CMBs incidence was 14.3% in all participants,lesions were located mostly in lobes.Aged,male and low total cholesterol level were the risk factors of CMBs (P<0.05).Patients with CMBs were more likely to suffer from acute cere-bral infarction (P<0.05).Two of 56 patients with new CMBs lesions located in lobar and mixed location respectively only.Conclusion Long term aspirin administration does not increase the risk of CMBs in non-hypertensive patients(P>0.05),the potential adverse effect of aspirin needs further investigation.
5.Value of FIB-4 and APRI index in evaluating the severity of liver fibrosis among patients with chronic hepatitis B
Lihua HE ; Zhilin NIU ; Yang YE
International Journal of Laboratory Medicine 2017;38(15):2076-2078,2081
Objective To explore the value of FIB-4 and APRI index on evaluating the severity of liver fibrosis among patients with chronic hepatitis B(CHB).Methods A total of 218 CHB were enrolled in the study.Based on the staging of liver fibrosis,the patients were divided into 2 groups as S0-S2 group(n=120) and S3-S4 group(n=98).Differences in clinical data,laboratory indexes,FIB-4 and APRI index were compared between two groups.The correlation between FIB-4 index,APRI index and liver fibrosis were analyzed by Spearman correlation test.Receiver operator curve(ROC) test was used to determine the evaluating value of FIB-4 index and APRI index for the severity of liver fibrosis.Results To evaluate the staging≥S2 of liver fibrosis,the value of FIB-4 index was better than APRI index(Z=1.998,P=0.046).And to evaluate the staging≥S3 and S4 of liver fibrosis,the value of FIB-4 index for evaluating the staging≥S3(Z=1.177,P=0.239) or S4(Z=0.267,P=0.789) was the same as APRI index.Conclusion FIB-4 index and APRI index are both effective on evaluating the severity of liver fibrosis among patients with CHB,but the value of FIB-4 index is better than APRI index for evaluating early liver fibrosis.
6.Congenital hypertrophic pyloric stenosis associated with intestinal malrotation in infants:clinical characteristics
Zhibin NIU ; Lixia HE ; Yong LI
Chinese Pediatric Emergency Medicine 2011;18(6):531-532
Objective To investigate the clinical characteristics and management of congenital hypertrophic pyloric stenosis (CHPS) associated with intestinal malrotation (IM) in infants.Methods The clinical data of 6 cases with CHPS and IM admitted in our hospital from Jan 2003 to Dec 2009 were reviewed,including clinical presentation,symptoms and information of imageology.Results Through barium meal examination and sonography,4 cases were diagnosed CHPS and IM,pyloromyotomy and Ladd's procedure were performed;two cases were diagnosed CHPS,only pyloromyotomy was done,secondary operation was performed for recrudescence of vomiting.All the cases were followed up for 1 to 3 years with good prognosis.Conclusion The etiology of CHPS with IM isn't clear.The diagnosis of IM may be delayed because the symptoms of IM can be masked by that of CHPS.It is helpful for diagnosis of this disease to have barium meal and sonography examination.Good prognosis will be achieved if prompt preoperative diagnosis and operation can be done.
7.The Clinical efficacy of combined therapy of adenosylcobalamin and transcutaneous eletrical nerve stimulation in the treatment of postherpetic neuralgia
Simeng NIU ; Lang HE ; Ying ZHAO
Chinese Journal of Rehabilitation Medicine 2010;25(3):232-235
Objective:To study the Clinical efficacy of combined therapy of adenosylcobalamin and transcutaneous eletrical nerve stimulation(TENS)in the treatment of postherpetic neuralgia(PHN).Method:Sixty patients with PHN were randomly divided into 3 groups:group A treated with TENS.group B treated with adenosylcobalamin,group C treated with adenosylcobalamin and TENS based on the management of groups A and B.The three groups had all been treated for 10d.Result:In observation,visual analogue scale(VAS)and 5 grade pain scores of group C were lower than those of groups A and B;VAS and 5 grade pain scores differences of group C were higher than those of groups A and B:The effective rate of group C(100%)was higher than that of groups A and B respectively.The differences were significant(P<0.05).Conclusion:Combined therapy of adenosylcobalamin and TENS is effective for the treatment of PHN and can alleviate pain and improve sleep.
8.THE EFFECT OF REMOTE SEGMENTAL ELECTRO-ACUPUNCTURE ON THE FLEXION REFLEX POTENTIAL AND THE DORSAL ROOT POTENTIAL
Wenyuan HE ; Heng ZHU ; Hanzhang NIU
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(02):-
Deseription was given of the effect of electric acupuncture of Neiguan point on the flexion reflex potential (FRP) of the hindlimbs and the dorsal root potential (DRP). On the intact and awake rats, the graded train stimulation (three pulses, 5ms interval, 0.5ms duration) was given to the sural nerve (a pure cutancous n(?), the C-FRP and C-DRP with their proper amplitudes (70~80% maximum) were obtained on the hamstring nerve (a group of nerves innervating the hindlimb flexors) and L5 dorsal root respectively. Then the electric acupuncture with low freqency and low intensity (4Hz, 1~3V)and low freqency and high intensity ( 4Hz, 20~40V), lasting 5 minutes, was given to the ipsilateral Neiguan point. After acupuncture, the amplitudes of both C-FRP and C-DRP decreased without change of their latebcues. The former one declined heavier and recovered slower than the latter one did. The A-FRP and A-DRP were not affected. Te result of the experiment suggests that the ipsilatcral Neiguan point cletric acupuncture can induce hindlimb's analgesia, the presynaptic inhibition might be involved in the mechanism of such analgesia.
9.THE FLEXION REFLEX POTENTIAL AND DORSAL ROOT POTENTIAL INDUCED BY STIMULATION OF THE CUTANEOUS NERVE IN THE RAT
Wenyuan HE ; Heng ZHU ; Hanzhang NIU
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
On the intact and awake rat, the flexion reflex potential (FRP)and dorsal root potential (DRP) induced by stimulation of the cutaneous nerve with graded intensity stimulations to the sural nerve (a pure cutaneous nerve) were observed, there appeared A-FRP and C-FRP one after the other on the hamstring nerve (a group of nerves innervating the hindlimb flexors), and A-DRP and C-DRP on the L_5 dorsal root at the same time. The threshold and the latencices of A-FRP and A-DRP had no differences, and had significant positive correlations. The amplitudes of A-FRP and A-DRP increased simultaneously with the increase of the stimulating intensity in certain range. The threshold and latency of C-FRP are larger than those of C-DRP. The threshold and the latency of the C-FRP and C-DRP showed significant positive correlation. The amplitudes of C-FRP and C-DRP increased simultaniously with the increase of the stimulating intensity in certain range. The train stimuli (three pulses, 5ms interval, 0. 5ms duration) induced larger FRP and DRP than those induced by single stimulus. Thus, FRP and DRP both have the property of temporal summation.
10.The clinical guide value of procalcitonin dynamic monitoring on antibiotics usage of patients with stroke associated pneumonia
Shuxiao ZHANG ; Yanliang NIU ; Gangrui HE
Clinical Medicine of China 2014;30(5):500-502
Objective To investigate the guidance value of procalcitonin(PCT) dynamic measurement on antibiotics usage of patients with stroke associated pneumonia(SAP).Methods One hundred and thirty-two cases with SAP were randomly divided into control group(64 cases with regular treatment) and observation group (68 cases with procalcitonin dynamic monitoring treatment).The treatment course of antibiotics in control group was according to antibiotics guidelines in China.Patients in observation group were given antibiotics for 5 days,stopped if the level of PCT was less than 0.25 μg/L,and continued to take if the level of PCT was more than 0.25 μg/L.The information were recorded including periods and cost of antibiotics usage,the periods and costs of hospitalization,clinical efficacy,mortality on the 30th day.The score of Institutes of National Institutes of Health Stroke Scale (NIHSS) and Barthel index (BI) were measured.Results (1) There were no significant differences in terms of age,gender,hypertension rate,coronary heart disease rate,diabetes rate,systolic blood pressure,the score of NIHSS in admission,pathogen and antibiotics between two groups (P > 0.05).(2) After 30 days treatment,there were no significant differences in terms of clinical efficacy(86.8% (59/68) vs.87.5%(56/64)),mortality (7.4% (5/68) vs.7.8% (5/64)),the score of NIHSS ((12.3 ±5.8) vs.(11.7 ± 4.5)) and BI (60.9 ± 22.3 vs 58.7 ± 21.6) between observation group and control group (P > 0.05).The periods of hospitalization in observation group was (20.1 ± 5.1) d,less than that of control group ((25.4± 6.0) d,t =5.479,P < 0.01).The costs of hospitalization in observation group was (8 ± 6) thousand yuan,significant lower than that of control group ((12 ± 8) thousand yuan,t =3.262,P < 0.01).The periods of antibiotics usage in observation group was (11.3 ± 2.6) d,less than that of control group ((14.4 ± 3.1) d,t =6.238,P < 0.01).The costs of antibiotics in observation group was (3 ± 1) thousand yuan,significant lower than that of control group ((5 ± 2) thousand yuan,t =7.331,P < 0.01).Conclusion PCT dynamic monitoring can guide the safe usage of antibiotics in SAP in terms of shortening the length of hospitalization,duration of antibiotics,and reducing the costs.