Objective　To investigate the existence, intensit y and persisting time of biologic activity of bronchoalveolar lavage fluid (BALF ) collected from dogs with acute severe smoke inhalation injury in early post-i njury stage. Methods　BALF was collected 1 h after the est ablishment of acute severe sawdust smoke inhalation injury in 5 dogs, and the fl uid was perfused into the lungs of Wistar rats in the amount of 5 ml/kg (gro up C). Normal saline (group A) and BALF from normal dog (group B) were perfused into the lungs of rats and served as control. The respiratory rate, PaO2, lung water content and the expanding stability of lungs in all rats were determ ined at the time points of 4,12 and 24 h after the purfusion. Results　 Compared with the rats in group A and B, the rats in group C had higher mortality, wider range in RR, higher lung water content, PaO2 decreased obviou sly and lower lung expanding stability. The rats in control groups showed sl i ght mechanic obstruction in their airways in the course of experiment. Meanwhile ，the rats in group C showed higher oxidative activities and lower total anti-o xidative activities in lung tissues. Conclusion　It is certai n that the BALF collected from dogs with acute severe smoke inhalation injury in early post-injury stage could induce obvious injury in lung structure of norma l rats, showing certain mechanic obstruction in small airways. The injuring act ivity of the BALF can be alleviated ultimately 24 h after the perfusion of the B ALF.

The gene of preferentially expressed antigen of melanoma(PRAME) is a gene marker of leukemia,which can be used to monitor the minimal residual disease,and can make us to comprehend the curative effect,prognosis,the grade of risk and can predict relapse.In addition,PRAME as a human tumour antigen,can be recognized by autologous cytolytic T lymphocytes,is a ideal target of immunotherapy in leukemia,and investigate the methylation level of PRAME gene CpG island,which can help us comprehend the relation of the mechanism of PRAME and leukemia.

Animals ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Liver Cirrhosis ; drug therapy ; genetics ; metabolism ; Liver Neoplasms ; drug therapy ; genetics ; metabolism ; Phytotherapy ; Procollagen ; biosynthesis ; genetics ; Tumor Suppressor Protein p53 ; biosynthesis ; genetics

Only limited species of animals with vomiting reflex are available for the vomiting animal models. From 1980s some new vomiting models have been developed, such as in ferret, which is convinced its vomiting behavior is closest to human being; Suncus murinus(house musk shrew)is the smallest mammal used in vomiting model and the most sensitive animal to motion sickness; rat, although without vomiting reflex, but consuming non-nutritive substance such as kaolin(pica) can be considered to be an index of nausea and vomiting; mink, from the same genus as ferret, can be a substitute of ferret in vomiting model. It should be noted that the sensitivity profile to different emetic stimuli is species-dependent.

Objective To analyze the clinical characteristics and curative effects of myoclonic epilepsy in children. Methods 35 cases were used to retrospectively study to analyze the medical history、clinical features and treatments.Results 7 patients(20%) had a family history of epilepsy while 11 cases(31.4%) had a history of febrile convulsion.The first onset was about at the age of 8.0?2.4. The first onset of myoclonic seizure and generalized tonic-clonic seizure would be put off and the diagnostic time would be delayed by 3.4 years.EEG induced by sleep and/or flash could raise the epileptic detectable rate.6 children had a tendency to be aggravated during the treatment.2 cases died of severe myoclonic epilepsy.28 patients(80%) were treated only by sodium vedproate and the effects were satisfying.Conclusion It is easily wrongly diagnosed and treated. Taking small dose of sodium vedproate drugs,the patients have better treatment effects.The children with severe myoclonic epilepsy have poor prognosis and high death rate.

Objective:To explore the possibility of fresh amniotic membrane transplantation for reconstruction of ocular surface with acute burn and evaluate the effect.Method:20 cases(24 eyes)due to acute burn of ocular surface were performed fresh amniotic membrane transplantation.All patients were followed up for 12 months to 26 months,the mean follow-up was(15?2)months.Visual acuity,epithelial healing,corneal transparency,corneal neovascularization and transplanted amniotic membrane were observed.Results:After fresh amniotic membrane transplantation,ocular surface was soon stable,immune rejection and secondary infection didn't occur.In 19 of 20 cases(23 eyes) the amniotic membrane autolyzed or disappeared with epithelized corneal surface at 14 to 28 days after operation.In one case(one eye) autolyzed corneal ulcer occurred at 12 days after operation.After another medication treatment was invalid,lamellar keratoplasty was performed.In other cases with AMT visual acuity keeps CF/5cm.Conclusion:Fresh amniotic membrane transplantation can lighten corneal inflammation in acute course of burn,can prevent ocular surface from ulcer and deliquescion,can fasten the stabilization and reduce corneal neovascularization,which provide a good basis for the following keratoplasty.With regard to severe eye burn,the effect of AMT is limited.

Objective:To detect the expression and significance of Dicer in gastric cell lines and gastric cancer tissue.Methods:Western-Blot and reverse transcriptase polymerase chain reaction(RT-PCR) were used to detect the expression of Dicer in normal gastric mucosa cell line GES-1,human gastric cancer cell line SGC-7901,25 surgical removed gastric cancer tissue and paired para-tumor tissue.Results:1.The expression of Dicer in human gastric cancer cell line SGC-7901 was lower than that in normal gastric mucosa cell line GES-1(P

Objective:To evaluate the effective way to lower the intraocular pressure(IOP) in the sudden attack of angle closure glaucoma.Methods:The treatment and effects of 105 patients suffering the sudden attack of angle closure glaucoma from June of 2001 to June of 2006 in my hospital were analyzed retrospectively.Result:62 eyes underwent therapy of medcine.There were 32 eyes of effective cure,with rate of 51.6%.43 eyes underwent therapy of anterior chamber penetration.There were 31 eyes of effective cure,with rate of 72.1%.There were significant differences between the two groups(?2=4.43,P

Objective: To investigate the expression of Skp2,c-Myc and P27kip1 protein,their association with clinicopathological features and the correlation between one and another.Method:Skp2,c-Myc and P27kip1 protein were examined by using immunochistochemical staining method in 20 cases of normal colorectal mucosa,20 cases of colorectal adenomatous polyp and 60 cases of colorectal cancer,and analysed the relationship with clinicopathologic features and between Skp2 with c-Myc and P27kip1 protein.Results:The positive rates of Skp2,c-Myc and P27kip1 protein in colorectal cancer were 71.67%,66.67% and 21.67% respectively.Moreover,the expression levels of Skp2 and P27kip1 protein in colorectal cancer were significantly associated with the pathologic grade,invasion deep,clinical stage and lymph node metastasis,c-Myc associated with the invasion deep,clinical stage and lymph node metastasis,but were independent of age and sex.Besides,in colorectal cancer,the expression levels of Skp2 and P27kip1 protein were inversely correlated,and the expression levels of Skp2 and c-Myc protein were positively correlated.Conclusions:The expression of Skp2,c-Myc and P27kip1 protein are associated with occurrence and development in colorectal cancer through their alteration,which is very important for assessment of malignancy degree and prognosis in colorectal cancer,and detection cancer of their expression provides theoretical basis for targetting therapy of colorectal.

Objective:To construct the expression vector pSuper.Hsp90?/pSuper for further research of the Hsp90?.Methods: In accordance with the Hsp90? sequence in gene bank,three sequences respectively targeting Hsp90? were cloned into the RNA expression vector pSuper.Results:It was confirmed by enzyme digestion,and electrophoresis that the construction of the recombinant expression vector pSuper-Hsp90?1,pSuper-Hsp90?2 and pSuper-Hsp90?3 was successfu1.pSuper-Hsp90?1 and pSuper-Hsp90?2 were successfu1 by sequencing.The expression of Hsp90? mRNA in HEK293 cells with pSuper-Hsp90?1,pSuper-Hsp90?2 and pSuper-Hsp90?3 decreased,and pSuper-Hsp90?1 notably decreased.Conclusion:The recombinant plasmid against Hsp90? RNAi can be successfully constructed in vitro,and pSuper-Hsp90?1 is the possible optimal target.