Animals ; Animals, Newborn ; Electrophysiology ; Female ; Membrane Potentials ; physiology ; NAV1.3 Voltage-Gated Sodium Channel ; Nerve Tissue Proteins ; genetics ; physiology ; Oocytes ; metabolism ; physiology ; Protein Subunits ; genetics ; physiology ; Rats ; Rats, Sprague-Dawley ; Sodium Channels ; genetics ; physiology ; Xenopus

OBJECTIVETo examine the effect of deglycosylation on gating properties of rNav1.3.

METHODSrNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control.

RESULTSCompared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV.

CONCLUSIONGlycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.

Animals ; Electric Conductivity ; Electric Stimulation ; Gene Transfer Techniques ; Glycosylation ; drug effects ; Homeostasis ; drug effects ; physiology ; Ion Channel Gating ; drug effects ; physiology ; Membrane Potentials ; drug effects ; physiology ; NAV1.3 Voltage-Gated Sodium Channel ; Nerve Tissue Proteins ; physiology ; Oocytes ; Patch-Clamp Techniques ; Sodium Channels ; physiology ; Static Electricity ; Tunicamycin ; pharmacology ; Xenopus