Recently, a severe slime mold infestation affected oriental melon plants in fields in Chilgok county, Gyeongbuk province, Korea. Specimens were collected from the fields and examined for identification. A species of Myxomycetes, Fuligo gyrosa, was identified based on its morphological characteristics. This is the first report that F. gyrosa causes slime mold of oriental melon.
Cucurbitaceae ; Fungi ; Korea ; Myxomycetes

Specimens collected from sweet potato plants with slime mold symptoms in fields in Daejeon, Korea were examined. Two species of Myxomycetes, Fuligo septica and Stemonitis herbatica were identified based on their morphological characteristics. This is the first report that the two species of Myxomycetes cause slime mold of sweet potato in Korea.
Fungi* ; Ipomoea batatas* ; Korea ; Myxomycetes*

In this study, we examined myxomycetes that developed in moist chamber cultures of substrata material collected from Kütahya and Konya provinces in Turkey. We collected bark samples from living trees, plant litter from the ground, standing dead wood or stumps, and downed and decayed wood or bark and placed them in the moist chamber cultures. We identified 36 species belonging to 12 genera of myxomycetes. The species determined are listed, and four new records, Didymium balearicum Ing, Macbrideola oblonga Pando & Lado, Paradiacheopsis erythropodia (Ing) Nann–Bremek. and Perichaena pedata (Lister & G. Lister) G. Lister, are added to the mycoflora of Turkey.
Ecology* ; Myxomycetes* ; Plants ; Trees ; Turkey ; Wood

In a large number of a1lergic cases, inhalant allergens, particularly airborne pollens and mo1ds am important causative, triggering agents in respiratory, even in skinanergy. It is essential for diagnosis and treatment of airborne allergy to survey the airborne pollen and mo1d in the patient's living area. Rotorod samp1er was installed on the well-ventilated rooftop of Hanyang Kuri Hospital in Kuri-shi, Kyunggi-do as the vicinity of Seoul. Airborne allergens were collected from October 1, 1995 to September 30, 1996 daily and were stained with Calberla`s fuchsin and were identified, counted under the light microscope (400Xfor pollen, 1000Xfor mold). The data were compared with the local daily weather report. The airborne pollen was found from 12th February to 22nd December. The maximal period for pollen collection was 7th-25th May (Maximal count:492 grains/ August. There were Alnus, Betula, Pinus, Quercus, Acer, Ulmus, PopuluS, Cupressaceae, Ginkgo, Corylus, Salk in the tree season from middle of February to middle of July. The grass season ranged from middle of March to end of November. There were Artemisia, Ambrosia, HumuluS, Chenopodium-Amaranthus, rumex, Plantago, Trifoium, Humulus, Chrysanthemum, Cyperus in the weed season ranged from middle of July to end of December. Airborne molds were collected all through the year except JanuaIgamma but, counted maximally from the middle of June to end l August. There were CladosPorium, Alternaria, ascospore of Leptospheria, Periconia, Myxomycete, Drechs1era, Rust urediniospores, Basidiospore, ToruIa, Xylariaceae, IOidimn, Nigrospom, Stempi1y1ium, Amphispheda, Fusarium, Aspergil1us. In Korea, there are great number of the allergic patients, but we have had a little records and data about study of relationship between the allergic diseases and airborne environmental allergens. we will reported those results completely in future.
Acer ; Allergens ; Alnus ; Alternaria ; Ambrosia ; Artemisia ; Betula ; Chrysanthemum ; Cladosporium ; Corylus ; Cupressaceae ; Cyperus ; Diagnosis ; Fungi* ; Fusarium ; Ginkgo biloba ; Gyeonggi-do* ; Humans ; Humulus ; Hypersensitivity ; Korea ; Myxomycetes ; Pinus ; Plantago ; Poaceae ; Pollen ; Populus ; Quercus ; Rosaniline Dyes ; Rumex ; Seasons ; Seoul ; Trees ; Ulmus ; Weather

The plasmodium of Physarum polycephalum is a suitable eukaryotic cell for cell cycle investigation, but there is no compatible transient expression system for the plasmodium. Using the promoter and terminator of ardC actin of Physarum polycephalum substituted the CMV IE and SV40 polyA of plasmid pDsRedl-N1, using cassette PardC-MCS-DsRed1-TardC substituted the cassette PardC-hph-TardC of plasmid pTB38, we constructed plasmids pXM1 and pXM2 for transient expression of red fluorescent protein (RFP) in Physarum polycephalum respectively. After reconstituting the transcription elongation factor homologous gene (pelf1) of Physarum polycephalum into the pXM2, we generated a plasmid pXM2-pelf1. After the plasmid pXM1, pXM2 and pXM2-pelf1 were electroporated into the plasmodium of Physarum polycephalum, we observed optimum RFP and PELF1-RFP expression under fluoroscope and confocal microscope between 24-48 h after electroporation, and found that ELF1-RFP expression was accumulated in nucleus of microplasmodium, the optimum electroporation parameters were 40 V/cm electric field, 1 ampere current, and 70 micros electric shock time. The results suggest that this expression system is qualified for transient expression of specific protein in plasmodium of Physarum polycephalum.
Actins ; genetics ; metabolism ; Electroporation ; Luminescent Proteins ; biosynthesis ; genetics ; Physarum polycephalum ; genetics ; metabolism ; Plasmids ; genetics ; metabolism ; Transcriptional Elongation Factors ; genetics

A known species, Physarum melleum, was found fruiting on living leaves of Dendrobium candidum, which was collected in China in 2004. Its morphological characters were revealed by light microscopy (LM), environmental scanning electron microscopy (ESEM) and scanning electron microscopy (SEM). Character variations were distinguished by its olive-yellow peridium and its always thinner capillitium containing globulose granular material between the large calcareous nodes. The calcium carbonate granules, deposited on stalks, peridium and hypothallus as well as within stalks, were globose and smooth.
Animals ; China ; Dendrobium ; ultrastructure ; Microscopy, Electron, Scanning ; Physarum ; ultrastructure ; Plant Leaves ; ultrastructure