We have studied the genetic differences among four isolates of Trichinella including a new strain of Trichinella spiralis (ISS 623) recently found from a human case who took a badger in Korea. Because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. It was analysed by PCR-RFLP analysis of the rDNA repeat that can readily distinguish a species or strain from others. Isolated genomic DNA of each isolate of Trichinella larvae was amplified with ITS1 specific primers and digested with restriction endonucleases. The PCR product of ITS1 was confirmed using Southern blot analysis to be a 910 bp fragment. The restriction fragments of each isolate had variable patterns when it was digested with Rsa 1 only. According to the RFLP patterns, the estimated genetic divergence between each isolate was different. In conclusion, four isolates of Trichinella including a new strain of T. spiralis obtained from a Korean patient may have genetic differences in the ITS1 region and the Shanghai isolate was genetically more similar to the Japanese unknown isolate than others in the ITS1 region.
Animals ; *DNA, Helminth ; *DNA, Ribosomal ; Human ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Repetitive Sequences, Nucleic Acid ; Trichinella spiralis/*genetics/isolation & purification

The authors analysed statistically 186 cases of the glaucoma patients among 20,785 out-patients who visited to our eye clinic from Jan. 1957 to Dec. 1969. The incidence of the glaucoma was 0.895% which was remarkably lower than the other reports. The following results were obtained. 1) Among the 186 cases, there were 85 male and 101 female patients. 2) There was no significant difference in left and right eye. 3) 51-60 year age group was prevalent (49 cases). 4) The age group over 40 occupied 61.3%. 5) The open angle glaucoma was the highest incidence.
Female ; Glaucoma* ; Glaucoma, Open-Angle ; Humans ; Incidence ; Male ; Outpatients

Reviewing the results of the blood cultures performed at Yeungnam University Hospital during 4-year-period through January, 1, 1984 to December 31, 1987, the following results were obtained. 1) Out of 8083 blood specimens cultured microorganisms grew in 582 specimens with positivity rate of 7.20%. Polymicrobial bacteremia was found in 16 patients. 2) Among 582 positive specimens, Gram-positive cocci grew in 189 specimens, and Gram-negative bacilli, in 393 specimens. Clinically significant microorganisms consisted of 82 Staphylococcus aureus, and 20 Streptococcus species in Gram-positive cocci group, 80 Salmonella typhi, 72 Escherichia coli, 72 Salmonella paratyphi A in Enterobacteriaceae, and 46 Pseudomonas cepacia, and 16 Pseudomonas aeruginosa in glucose non-fermenting microorganisms. 3) Increasing incidence of Serratia, Acinetobacter and Pseudomonas species as major nosocomial infection source is noteworthy. They showed increased tendency from 6.3% of 1984 to 17.7% of 1987 of total positive blood cultures. 4) High isolation rate of Pseudomonas species and Aeromonas hydrophila was noted in summer, while Salmonella typhi showed high prevalence from May to September and in January. 5) In susceptibility tests of isolated organisms, staphylococcus aureus was sensitive to basic antimicrobial agents except for ampicillin. The glucose non-fermenting microorganisms showed high resistance to basic antimicrobial agents in 32.2%. In conclusion, considering the relatively higher incidence of growth of Staphylococcus epidermidis than ideal level indicates that sampling technique should be improved. Secondly, all the hospital staffs in cooperation with Hospital Infection Committee are desirable to pay efforts to decrease the nosocomial infection.
Acinetobacter ; Aeromonas hydrophila ; Ampicillin ; Anti-Infective Agents ; Bacteremia ; Burkholderia cepacia ; Cross Infection ; Enterobacteriaceae ; Escherichia coli ; Glucose ; Gram-Positive Cocci ; Humans ; Incidence ; Prevalence ; Pseudomonas ; Pseudomonas aeruginosa ; Salmonella paratyphi A ; Salmonella typhi ; Serratia ; Staphylococcus aureus ; Staphylococcus epidermidis ; Streptococcus

No abstract available.
Aged* ; Humans

We measured fasting Serum Angiotensin-Converting Enzyme (SACE) in 100 healthy controls and 75 coal worker's pneumoconiosis (CWP) patients by a commercial kits (ACEcolor®, Fujirio Inc., Japan) and evaluated this manual method. The linear range extends to an activity of 80U/L. Precision on a commercial control serum (ACE control-N®, Sigma Co.) with a mean value of 9.47U/L yielded a within-run and between-run CVs are 5.6% (N=15) and 6.9% (N=14) respectively. Save in 75 CWP was 20.3±5.7U/L (mean±s.d.); higher than in healthy controls (13.4±3.9U/L, P<0.01). No correlation was found between SACE, sex, and age. The results suggest that the measurement for SACE and follow-up SACE in coal workers may be a useful diagnostic tools for CWP.
Anthracosis* ; Coal* ; Fasting ; Follow-Up Studies ; Humans ; Methods

Pure red cell aplasia in uncommon disorder characterized by finding of anemia, absence of nucleated red blood cell in the marrow, absence of reticulocytes in the peripheral blood and normal peripheral platelet and leukocytes counts. We experienced one case of pure red cell aplasia associated with hemolytic anemia characterized by hemoglobinuria, reticulocytopenia, and erythroid hypoplasia of the bone marrow. The cause of the illness was not definitely identified, but we concluded that this patient had simultaneous occurrence of PRCA and hemolytic anemia following administration of diphenylhydantoin after craniotomy rather than virus or bacteria induced. The simultaneous occurrence of PRCA and hemolytic anemia in uncommon and the mechanism for diphenylhydantoin induced PRCA and hemolytic anemia is unclear.
Anemia ; Anemia, Hemolytic ; Bacteria ; Blood Platelets ; Bone Marrow ; Craniotomy ; Erythrocytes ; Hemoglobinuria ; Humans ; Leukocytes ; Phenytoin ; Red-Cell Aplasia, Pure* ; Reticulocytes

No abstract available.
Carcinoma, Hepatocellular*

Serum lactate dehydrogenase isoenzyme are separated 5 fractions (LD1-LD5) in normal serum by agarose electrophoresis. An unusual band on the cathodic side of LD5 isoenzyme has been found on lactate dehydrogenase electrophoresis is of serum, called LD6, and the presence of which signifies a poor prognosis for the patients of myocardial infarction. In recent, we have experienced LD6 in two patients with myocardial infarction. Cardiac arrest was noted within 24 hours after exhibition of LD6 extra band and they did not recover from their illness.
Electrophoresis ; Heart Arrest ; Humans ; L-Lactate Dehydrogenase ; Myocardial Infarction* ; Prognosis ; Sepharose

No abstract available.

PURPOSE: To investigate the efficacy of percutaneous embolization of hypervascular VX-2 carcinoma in the retroorbital area of the rabbits using Histoacrylic Blue(N-butyl 2-cyanoacrylate, NBCA) and to establish as an experimental model. MATERIALS AND METHODS: Retroorbital VX-2 carcinoma was made in 10 rabbits by injection of carcioma cells. At 2 and 4 weeks following the tumor cell injection, carotid angiography was performed before and after percutaneous embolization using 50% NBCA diluted with Lipiodol. Doppler ultrasonography was also performed to evaluate the vascularity of the mass. Light microscopic examination was used to evaluate the effect of embolization on the tumor mass. RESULTS: Angiography following percutaneous embolization showed filling of embolic materials in the tumor beds. Ultrasonographic findings showed tumor necrosis and increased Doppler signal in the tumor. Histopathological examinations showed extensive necrosis of tumor cells with remaining viable cells surrounding the capillaries. There were no NBCA emboli in arterioles and capillaries of tumor beds in multiple sections. CONCLUSION: Since 50% diluted NBCA used as percutaneous embolus into the retroorbital VX-2 carcinoma dose not get into small blood vessels of tumor beds. using NBCA in lower concentration in the hypervascular tumor would be the next step to figure out its usefulness. This study also serves as an experimental model of percutaneous embolization of hypervascular tumor.
Angiography ; Arterioles ; Blood Vessels ; Capillaries ; Embolism ; Enbucrilate* ; Ethiodized Oil ; Models, Theoretical ; Necrosis ; Orbit* ; Rabbits* ; Ultrasonography, Doppler