No abstract available.
Animals ; Calcitonin Gene-Related Peptide* ; Calcitonin* ; Rats* ; RNA, Messenger*

No abstract available.
Transplants* ; Urinary Incontinence*

Magnetic resonance imaging(MRI) is accepted as a useful tool for the diagnosis of injury of the knee recently. The anterior cruciate ligament tear is accompanied by the damage of the osseous and soft tissue of the knee. The occult osseous lesion is found on magnetic resonance imaging as bone density change that is not found on the simple radiograph. From Jan. 1992 to Apr. 1994, magnetic resonance imaging was performed on 44 patients with acute anterior cruciate ligament tears. MRI was done within 6 weeks of the index anterior cruciate ligament injury. The criteria of decreased signal intensity on the Tl weighted image and increased signal intensity on the T2 weighted image was applied for the incidence assessment of the occult osseous lesion. After arthroscopy with or without ACL reconstruction, follow-up simple radiograph was checked every 3 months. Occult osseous lesions were documented in 32(73%) of the 44 patients. Of the 32 patients with bony lesion, 81% had lesions of the lateral compartment. Lateral femoral condyle was involved in 15 cases(46.9%), and lateral tibial plateau in 22 cases (68.8%). Follow-up X-ray revealed no joint deperession in all of the 32 patients with the occult osseous lesion. This result suggests that there is a high correlation between the occult osseous lesion of lateral compartment of the knee on magnetic resonance imaging and anterior cruciate ligament tear. The presence of the occult osseous lesion in the patient with acute anterior cruciate ligament tear did not affect rehabilitation and weight bearing.
Anterior Cruciate Ligament ; Arthroscopy ; Bone Density ; Diagnosis ; Follow-Up Studies ; Humans ; Incidence ; Joints ; Knee ; Magnetic Resonance Imaging ; Rehabilitation ; Tears ; Weight-Bearing

In Korea, all domestic made test systems for detecting antibodies in HIV-1 contain the antigens from human immunodeficiency type 1 (HIV-1) subtype B. However, because HIV-1 subtype O is significantly different in amino acid sequences from all other subtypes of HIV-1, there has been a need for developing a test for detecting antibodies in subtype O. For this purpose, the entire nucleotide sequence corresponding to the extracellular domain of the transmembrane glycoprotein of HIV-1 subtype O was synthesized with consideration of Escherichia coli cordon usage. Various regions of the extracellular domain were cloned into E. coli expression vectors and tested for levels of protein production. The nucleotide sequence, named ECTM, that can encode a 129 amino acid-long peptide, was found to be expressed at a high level in E. coli. The protein of approximately 17 kDa specifically reacted with sera from individuals infected with HIV-1 subtype O. The ECTM protein was purified to near homogeneity by the CM-T gel chromatography, using concentrated, denatured inclusion bodies. In Western blot analysis, the purified viral antigen reacted with sera from individuals infected with subtype O more efficiently than subtype B. The enzyme linked immunoabsorbent assay (ELISA) system was developed using the subtype O viral protein and compared with the commercially available kit lacking the antigens from subtype O. The ELISA kit containing the subtype O antigen ECTM alone efficiently reacted with sera from individuals infected with subtype O. The subtype O antigen-containing kit produced a positive absorbence even when sera were diluted 512-fold, suggesting a high sensitivity. The commercially available kit also reacted with subtype O sera, but produced a negative result at a dilution of 8-fold. Our results suggest that the currently available kit may not be able to efficiently detect subtype O sera and that the viral protein developed in this study may be added to the current system to maximize the detection of sera from individuals infected with subtype O.
Amino Acid Sequence ; Antibodies ; Base Sequence ; Blotting, Western ; Chromatography, Gel ; Clone Cells ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; Glycoproteins ; HIV* ; HIV-1* ; Humans* ; Inclusion Bodies ; Korea ; O Antigens

No abstract available.
Knee* ; Magnetic Resonance Imaging*

No abstract available.
Humans ; Infant, Newborn*

PURPOSE: To assess the relationship between the internal diameter of the nasolacrimal duct and the success rate of silicone tube intubation in incomplete nasolacrimal duct obstruction patients with epiphora. METHODS: Dacryocystography was performed 80 patients with symptoms of epiphora and the anteroposterior (AP) diameters and the lateral diameters of the nasolacrimal ducts were measured. These measurements were compared between the group of patients who demonstrated improvement after the operation, and the group without symptom improvement. RESULTS: The mean length of the nasolacrimal duct in the group with nasolacrimal stenosis was 11.7+/-0.1 mm, the mean lateral diameter was 1.0+/-0.2 mm, and the mean AP diameter was 1.1+/-0.1 mm. The AP diameter of the symptomatic nasolacrimal duct in the group with successful postoperative results was 1.1+/-0.1 mm, and 0.9+/-0.1 mm in the group with unsuccessful results. The group with successful postoperative results had an average of 18.1+/-7.0% stenosis in the side of the eye with nasolacrimal duct stenosis compared with the asymptomatic side, and the group with unsuccessful postoperative results had an average of 34%+/-3.6% stenosis. CONCLUSIONS: In cases with nasolacrimal duct stenosis, the success rate of silicone tube intubation was high when the AP diameter of the nasolacrimal duct was more than 1.1+/-0.1 mm and when the stenosis was less than 18.1+/-7.0% in the symptomatic side compared with the opposite side. These results are expected to help predict the success rates when preoperatively planning silicone tube intubation.
Constriction, Pathologic ; Eye ; Humans ; Intubation ; Lacrimal Apparatus Diseases ; Nasolacrimal Duct ; Silicones

PURPOSE: A comparison analysis of the clinical characteristics and treatment results of congenital NLD obstruction. METHODS: The present study involved 216 pediatric patients (258 eyes) diagnosed with and treated for congenital NLD obstruction. Treatment was applied step by step starting with conservative massaging, lacrimal probing, and silicone tube intubation. RESULTS: The treatment results of congenital NLD obstruction in pediatric patients under 12 months of age showed an 87.3% success rate after an average of 4.3 months of treatment in the group using lacrimal sac massage and antibiotic eye drops (conservative treatment). The group treated by correct massaging techniques showed a 93.6% success rate after an average of 3.8 months of treatment. The success rate of lacrimal probing, applied to patients not showing improvement after massage therapy was 77.8%. Pediatric patients not showing improvement after lacrimal probing underwent silicone tube intubation. All patients showed improvement except in 1 case that had an early silicone tube dislocation. DISCUSSION: A step-by-step approach is effective in treating congenital NLD obstruction patients, and correct lacrimal massage techniques improve the success rate during conservative therapy. The period of conservative treatment and number of probing times did not have a statistically significant correlation with the success rate of lacrimal silicone tube intubation. Silicone tube intubation showed a high success rate in patients sustaining the silicone tube for a minimum of 2.1 months.
Dislocations ; Humans ; Intubation ; Massage ; Nasolacrimal Duct ; Ophthalmic Solutions ; Silicones

No abstract available.
Acetazolamide* ; Aneurysm* ; Brain* ; Humans ; Perfusion* ; Subarachnoid Hemorrhage* ; Tomography, Emission-Computed, Single-Photon*

A clinical observation was made on 48 cases of benign prostatic hyperplasia, admitted during the period from January 1975 to June 1981 were studied clinically in the Department of Urology, Seoul Adventist Hospital. The results are summarized as follows. 1. The incidence of benign prostatic hyperplasia was 5.88% of total in-partients (1,227) and there was an increasing tendency of annual distribution from 3 patients (3.89%) in 1975 to 10 patients (11.62%) in June 1981. 2. The youngest age was 50 and the oldest 83. The mean age of 48 cases was 69.65 years and the seventh decade was most prevalent (54.17%). 3. The presenting symptoms of 48 cases, 43 cases (89.6%) were dysuria, 32 cases (66.7%) were acute urinary retention, 5 cases (52.1%) were frequency, 22 cases (45.8%) were hematuria, 17 cases (35.4%) were lower abdominal pain, 6 cases (12.5%) were pyuria, 5 cases (10.4%) were incontinence and 3 cases (6.3%) were nocturia. 4. The mean duration of symptom was 2.28 years. 5. The mean volume of residual urine was 502 ml. 6. Pyuria revealed in 28 cases (58.3%), Hematuria in 39 cases (81.3%), Culture positive in 24 cases (50.0%), Azotemia in 9 cases (18.8%). 7. Performed I.V.P. in all cases, and hydronephrosis was found in 7 cases (14.6%). 8. Performed endoscopy in all cases, and most common finding was trabeculation (81.3%). 9. Associated diseases present were cardiovascular in 5 cases, respiratory in 6 cases, gastrointestinal tract in 7 cases, urologic in 18 cases, and miscellaneous in 7 cases. 10. The mean weight of resected prostatic tissue was 9.75 gm. 11. The mean period of urethral catheter drainage was 6.5 days. 12. The mean period of postoperative gross hematuria was 5.25 days. 13. The mean period of postoperative hospitalization was 7.83 days. 14. Postoperative complications were cystitis in 22 cases (45%), temporary incontinence in 8 cases (16.7%), epididymitis in 3 cases (6.3%), delayed bleeding in 3 cases (6.3%), urethral strictures, vesical hematoma and delayed wound healing in each 2 cases (each 4.2%) and pyrexia in 1 case (2.1%). 15. Retrograde urethrography was performed on postoperatively about 1 month later. The mean length of the prostatic urethra was 5.72 cm preoperative, postoperative length was shortened to 4.77 cm.
Abdominal Pain ; Azotemia ; Cystitis ; Drainage ; Dysuria ; Endoscopy ; Epididymitis ; Fever ; Gastrointestinal Tract ; Hematoma ; Hematuria ; Hemorrhage ; Hospitalization ; Humans ; Hydronephrosis ; Incidence ; Male ; Nocturia ; Postoperative Complications ; Prostatic Hyperplasia* ; Pyuria ; Seoul ; Transurethral Resection of Prostate* ; Urethra ; Urethral Stricture ; Urinary Catheters ; Urinary Retention ; Urology ; Wound Healing