No abstract available.

No abstract available.

The authors experienced a case of basosquamous cell carcinoma occuring in the eyelid, which was confirmed by the biopsy. Histological findings showed the infiltrating nests of malignant squamous cell with nests of basal cell infiltrating into the dermis. A huge ulcerating, infected lesion with irregular indurating border involved the entire lid and extended onto the orbital margin and nose with the nasolacrimal duct exposed. Because of the unusual case, it was felt that literatures review would be instructive.
Biopsy ; Dermis ; Eyelids* ; Nasolacrimal Duct ; Nose ; Orbit ; Ulcer

The retinal and choroidal blood vessels respond independently to the abruptly increased arterial pressure due to their differences in the anatomic and physiologic properties, which induce hypertensive retinopathy and hypertensive choroidopathy respectively. The authors reviewed the fluorescein angiogram retrospectively to observe the ischemic changes of the choroid in 15 cases of hypertensive choroidopathy. The ischemic changes of the choroid in hypertensive choroidopathy were characterized by generalized or sectorial filling delay which was followed by staining or leakage of dye. These findings suggest that the choroidal circulation may lead to the sectorial and generalized ischemic conditions following the abruptly increased arterial pressure due to their differences in the anatomic structures. The fluorescein angiographic findings in the hypertensive choroidopathy depend on both the degree of the circulatory disturbance and the levels of the affected choroidal vessels.
Arterial Pressure ; Blood Vessels ; Choroid ; Fluorescein Angiography* ; Fluorescein* ; Hypertensive Retinopathy ; Retinaldehyde ; Retrospective Studies

PURPOSE: To use impression cytology to examine the structural changes in corneal epithelial cells infected with the herpes simplex virus in rabbit eyes. METHODS: Corneal surfaces of 7 rabbits were scratched using a 25-gauge needle. Herpes simplex virus (type 1, Kos strain) was inoculated to the injured cornea. As the corneal diseases were observed using slit lamp biomicroscopy, impression cytology was performed for 18 days after inoculation. Specimens were stained with hematoxylin-eosin and examined using optical microscopy. RESULTS: Corneal lesions consisted mainly of round epithelial cells, inflammatory cells, ballooning cells, multinucleated giant cells, and various inclusion bodies. Over time, the corneal epithelial cells peeled away as a result of corneal edema in the corneal lesions. Dendritic lesions were also observed. In the recovery phase, the number of detached cells and infiltrated inflammatory cells decreased. CONCLUSIONS: It was presumed that dendritic lesions might have been formed at the scratched cornea region, thereby aggravating the epithelial cells falling off as a result of the infiltration of inflammatory cells. These cytopathologic effects occur in experimental herpes simplex keratitis.
Animals ; Cercopithecus aethiops ; Cornea/*pathology ; Cytological Techniques ; Epithelium, Corneal/pathology ; Keratitis, Herpetic/*pathology ; Rabbits ; Time Factors ; Vero Cells

PURPOSE: To investigate the replication of HSV within cultured cell and axonal transport of HSV within the axon of the ciliary nerve following the injection of HSV into a cultured ciliary nerve. METHODS: The explant of the ciliary nerve was cultured with a medium containing nerve growth factor for 30 days when the suspension of HSV-1 (Kos strain) was introduced into the culture dish to co-culture with the ciliary nerve. The ciliary nerve was examined with transmission electron microscopy 30 days after culture and 6 days after co-culture with HSV. RESULTS: The ultrastructure of the explant of the ciliary nerve co-cultured with HSV showed that the viral capsid acquired a viral envelope and viral core, and a capsid and inclusion body within the nucleus. The enveloped virus was scattered within the vesicles of the cytoplasm. The virus-like particles were identified at the axonal fibers. CONCLUSIONS: The co-culture of the explant of the ciliary nerve and HSV showed the replicative process of the HSV within the cultured cell. The virus-like particles within the axon showed the evidence axonal transport of the virus under culture conditions.
Axonal Transport* ; Axons ; Capsid ; Cells, Cultured ; Coculture Techniques ; Cytoplasm ; Herpes Simplex* ; Herpesvirus 1, Human ; Inclusion Bodies ; Microscopy, Electron, Transmission ; Nerve Growth Factor ; Simplexvirus*

In this study, the oxygen permeabilities and diffusion rates of several soft contact lenses were measured and compared. The hydrophilic contact lenses used in this experiment were composed of four different types, 27, 32, 48 and 70 per cent of water content, respectively. Oxygen permeability rates were determined by using oxygen diffusion apparatus ctlmposed of two chamber diffusion system under a partial pressure gradient of approximately 5:1. Serial samples of 1ml were removed from the each chamber at 30 minutes interval and the Po2 in the samples was measured on a blood gas analyser. The oxygen flow rates of four different soft contact lenses varied from 99.8 micro l/cm2/hr to 292.1 micro l/cm2/hr, depending upon their water contents. The permeability characteristics was affected by the state of hydration of lens and the comparison of the oxygen flow rates showed that even the least permeable soft contact lens in this study was able to meet the corneal oxygen requirement.
Contact Lenses, Hydrophilic* ; Diffusion ; Oxygen ; Partial Pressure ; Permeability* ; Water

To evaluate the ultrastructure of cornea keratocyte infected by Herpes simplex virus, the cornea was excised from enucleated rabbit eyes. Cornea epithelium and endothelium of these was removed and cultivated in culture media. After 7 days, the Kos strain of Herpes simplex virus was inoculated into the cultured cornea keratocytes and co-cultivated until the cytopathic effect occurred. Cornea keratocytes was fixed in the 3%glutaraldehyde and examined with electron microscope. The infected cells were active cells and slightly more round appearance than normal cells. They had microvillies composed of cytoplasmic process protru-sions into collagen fibers. Virus particles was transmitted into neighboring cells easily since there was no barrier function of collagen fibers that was destroyed in the condition of culture. The nuclear changes of infected cells were marked :nuclear chromatin was degraded, condensated and displaced toward the nuclear membrane. There were many virus particles in the nucleus. There were also many degenerations in the cytoplasm, destructed numerous cytoplasmic organelles and many virus particles seen in the cytoplasm. The ultrastructural findings of keratocytes co-cultivated with Herpes simplex virus were identified from our result.
Chromatin ; Collagen ; Cornea ; Culture Media ; Cytoplasm ; Endothelium ; Epithelium ; Herpes Simplex* ; Microvilli ; Nuclear Envelope ; Organelles ; Simplexvirus* ; Virion

Keratic precipitates are deposits of material on the posterior surface of the cornea, which is a relatively common phenomenon in a variety of circumstances both physiological and pathological. Inflammatory cells and uveal pigment in the aqueous show a strong tendency to adhere to one another and to the corneal endothelium, thus forming fine or large deposits. We observed the several kinds of keratic precipitates on the corneal endothelium by flat preoparation method. The character of the keratic precipitates observed in this study was composed of inflammatory cells, erythrocyte, pigment granules derived from the breakdown of red blood cells. In view of the accumulation of the pigment granules into the cytoplasm, it seemed that the endothelium might participate in phagocytosis or secondary changes in the various corneal disease.
Cornea ; Corneal Diseases ; Cytoplasm ; Endothelium ; Endothelium, Corneal ; Erythrocytes ; Phagocytosis

In order to interprete the significance of the vacuolation found in the corneal endothelium, flat preparations of the corneal endothelium were made in human eyes aged from neonate to 72 year. The result were as follows. 1. The endothelial vacuoles were more frequent with the increase in the time of the post-mortem delay. 2. The endothelial vacuoles were more prevalent in aged. 3. The vacuoles in the endothelium were more easily formed in the peripheral area than the central area. 4. Gross vacuolation found in the corneal endothelium was shown to be the result of post mortem degeneration.
Endothelium ; Endothelium, Corneal* ; Humans ; Infant, Newborn ; Vacuoles*