No abstract available.

No abstract available.

This study was undertaken in an attempt to localize fibronectin and collagen type IV to the cultured retinal pigment epithelial cell by means of immunofluorescent staining and immunocytochemrcal method. Immunofluorescent staining and immunocytochemical methcds revealed fibronectin and collagen type IV localized on the extracellular membrane of the cultured retinal pigment epithelial cell. Ultrastructural immunocytochemical technique also revealed fibronectin associated with extracellular tissue. This study demonstrated that fibronectin and collagen type IV are an integral component of the extracellular matrix of the retinal pigment epithelial cell in vitro.
Collagen Type IV ; Epithelial Cells* ; Extracellular Matrix* ; Fibronectins ; Membranes ; Retinaldehyde*

This study was performed to observe the sequential charge of the morpholcgic characteristics in experimentally induced herpes simplex keratitis. Duration and morphology of corneal lesion following infection of rabbit cornea with the Kos strain of HSV-I were followed by a daily slit-lamp examination. Three types of virus inoculation methods were used such as scratching, deepithelialization, and intrastromal injection. Herpetic corneal lesions appeared 24 hours after inoculation with punctate and dendritic figures. They persisted up to 14 or 15 days. The characteristic finding in punctate herpetic keratitis was grouped, round-shaped, punctate lesion. When scratching method was emplyed, the most remarkable finding was the discontinuity of the lesion occurred along the scratching wound at relatively regular intervals. There was no difference in lesional morphology and duration between three inoculation methods.
Cornea ; Keratitis* ; Keratitis, Herpetic ; Wounds and Injuries

This study was performed to investigate the sequential changes of the retinal tissue in tissue culture condition. The human sensory retinal tissues were cultured for up to 2 weeks and 4 weeks, respectively. The initial changes showed the separation of the intercellular space and the consequent widening of the intercellular space with prolapse of cytoplasmic processes into the widened intercellular space. The internal limiting membrane was also separated from the inner retina, which led to the prolapse of the cytoplasm of the Muller cell. The growth of the Muller cell was most prominent during the 4-weeks' tissue culture period. These findings suggest that the Muller cell might contribute to the formation of cellular membrane in case of the defect of the internal limiting membrane in several pathologic conditions.
Adult ; Cell Membrane/ultrastructure ; Cells, Cultured ; Humans ; Male ; Middle Aged ; Neuroglia/*ultrastructure ; Retina/*ultrastructure

The 206 human embryonic and fetal eyes were conducted to investigate the structural change of the developing retina from Streeter age 13 to 40 week's gestational age with light microscopy. The development of the sensory retina was characterized by the appearance of the transient layer of Chievitz about 7 week's gestational age. The ora serrata was identified from the peripheral retina with the finding of the peripheral thinning around 13 week's gestational age. The transient layer of Chievitz was changed to a part of the inner plexiform layer around 15 week's gestational age. All retinal layers were apparent in structural characteristics around 19 week's gestational age and nearly complete in differentiation around 29 week's gest-ational age. During fetal stage, the retina was advanced in the region of the pos-terior pole than in the peripheral retina, the development of which was complete in differentiation about 36 week's gestational age.
Fetus* ; Gestational Age ; Humans* ; Microscopy ; Retina* ; Retinaldehyde

The authors experienced a case of basosquamous cell carcinoma occuring in the eyelid, which was confirmed by the biopsy. Histological findings showed the infiltrating nests of malignant squamous cell with nests of basal cell infiltrating into the dermis. A huge ulcerating, infected lesion with irregular indurating border involved the entire lid and extended onto the orbital margin and nose with the nasolacrimal duct exposed. Because of the unusual case, it was felt that literatures review would be instructive.
Biopsy ; Dermis ; Eyelids* ; Nasolacrimal Duct ; Nose ; Orbit ; Ulcer

The whole retina, except for the medullary fiber zone in a rabbit eye, is supplied by choroidal circulation. Therefore, the histopathological changes of the sensory retina due to choroidal circulatory disturbance in rabbits may be comparable to that of the human sensory retina in the case of ophthalmic artery occlusion. This study was carried out to evaluate the histopathological changes of the ischemic retina secondary to the occlusion of choroidal circulation. The experimental occlusion of all posterior ciliary arteries and anterior ciliary arteries in the horizontal rectus muscle of rabbit eyes was performed and the subsequent histopathological changes of the sensory retina were observed by transmission electron microscopy. The morphological changes of the sensory retina following the occlusion of the ciliary arterial system are as follows: severe loss of the inner and outer segments of the photoreceptor, mild to moderate degeneration of the ganglion cells, and excellent preservation of the Muller's cell fibers and the extension of the cytoplasmic villous processes to the cytoplasmic vacuolar spaces of other degenerated cells. These findings indicate that the Muller's fibers in the ischemic condition of retina might contribute to the formation of gliosis or scarring of a damaged retina.
Animals ; Arterial Occlusive Diseases/*complications ; Arteries ; Choroid/*blood supply ; Ciliary Body/*blood supply ; Ischemia/*etiology/pathology ; Rabbits ; Retina/*ultrastructure ; *Retinal Vessels

This study was performed to elucidate the structural changes of the attachment complex in the cultured cornea. The three normal corneal tissues were used in this study. The ultrastructural changes of the attachment complex were observed by electron microscopy in one corneal tissue which was not cultured and cultured for 6 days and two corneal tissues which were cultured 10 days and 20 days, respectively. The cornea cultured for 6 days showed changes in the electron density of the hemidesmosome. The basal lamina was focally detached from the cytoplasmic wall of the basal epithelial cells in the cornea cultured for 10 days. The anchoring fibrils within the nuded corneal stroma, which was cultured for 20 days, were markedly decreased in number. These findings suggest that the normal basal epithelial cell, hemidesmosomes and basal lamina might be the essential factors to maintain the networks of normal anchoring fibrils in corneal stroma.
Basement Membrane ; Cornea ; Corneal Stroma ; Cytoplasm ; Epithelial Cells ; Hemidesmosomes ; Microscopy, Electron

PURPOSE: To evaluate the morphological characteristics of the transitional zone between the corneal endothelium and the trabecular meshwork by flat preparation and electron microscopy. METHODS: The materials comprised 12 eyes examined by the flat preparation and 7 eyes by the electron microscopy. The specimens were derived from the transitional tissue between the corneal endothelium and the trabecular meshwork. The specimens in the flat preparation were stained with hematoxylin-eosin and examined by light microscopy. The specimens for scanning electronic microscopy (SEM) and in transmission electronic microscopy (TEM) were examined through routine processes. RESULTS: In the specimens examined by the flat preparation, unlike peripheral corneal endothelial cells, the endothelial cell nuclei in the transitional zone were overlapped and morphologically oval. On SEM, unlike typical hexagonality and tight interdigitation of corneal endothelial cells, the endothelial cells in the transitional zone were partially successive, spaced intercellularly, and morphologically irregular. On TEM, the endothelial cells in the transitional zone were partially successive. CONCLUSIONS: The loss of cell-cell contact of endothelial cells in the transitional zone may lead to the potential proliferation capacity of endothelial cells in the transitional zone under specific conditions. Therefore, further studies on the proliferation capacity of endothelial cells in the transitional zone are needed together with more research on cell biology.
Endothelial Cells ; Endothelium, Corneal* ; Microscopy ; Microscopy, Electron ; Trabecular Meshwork