This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.
Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; Concanavalin A/pharmacology ; DNA, Complementary/*chemistry ; Dogs/blood/genetics/*immunology ; Interleukin-3/chemistry/*genetics ; Interleukin-6/chemistry/*genetics ; Leukocytes, Mononuclear/chemistry/drug effects ; Molecular Sequence Data ; Open Reading Frames/genetics ; Protein Sorting Signals/genetics ; RNA/blood/genetics ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid

100 cases (99 male, 1 female) with defective co1or vigion but good vigual acuity, and no retinal diseases that could be detected by screening with Iehihara's test were ge1ectgd from 2708 cases (maIe 2382, female 326) that had undergone a physicaI examination at National Semil Hogpital. The 100 cages wgre examined using a printed sample of plates including Ishihara-Ohuma's test and Hahn's double 15-hue test. After thig test, the 100 cages were examined using Nagel's anomaloscope. The anomaloscope using the wavelengths 670nm and 546nm to mathch 589nm is the most accurate means of diagnosing defects of red and green vision. Results of examination in classifing the types of color defects using the plates of both Ishihara-Oguma's test and the Hahn's doume 15-hue test as well as the Nagel's anomaloscope were as follows: 1. In the total of 2708 persons examined, there were 2382 males and 326 females from this 100 cases of congenital color defects were discovered 99(4.16%) males and female 1(0.31%). 2. The classification and rates of types of congenital color defects were as followg: 99 ma1eg were classified with 12(12.1 %) protanopia, 35(35.4%) deutanopia, 9(9.1 %) protanomalia and 43 (43.4 %) deuteranomalia, on the other hand one ferna1e was found out deuteranomalia.
Classification ; Female ; Hand ; Humans ; Male ; Mass Screening ; Retinal Diseases

Asteroid hyalitis is an uncommon condition in which small, solid, stellate, spherical or disk-shaped bodies are suspended in a substantially normal vitreous. The opacities occurs in strands or as discrete bodies without orderly arrangement. These move when the eyes move but always return to their original position. The authors experienced one case of asteroid hyalitis associated with arteriosclerosis and hypertension. so we report this with brief review of the literature.
Arteriosclerosis ; Hypertension

No abstract available.
Paget Disease, Extramammary*

About 10% ~ 40% of patient with Marie-Strumpell ankylosing spondylitis have anterior uveitis. Recently, relationship between ankylosing spondylitis and anterior uveitis was discoverd by several studies of HLA-B27 antigen. Ankylosing spondylitis was found to be the commonest single etiological factor in analysis of 653 cases of uveitis seen in the Uveitis Clinic in United Kingdom. Ankylosing spondylitis is mostly found in male and most of their natures of uveitis are acute unilateral nongranulomatous anterior uveal involvement. Roentgenological findings of ankylosing spondylitis are characteristic demineralization and subchondral sclerosis of sacro-iliac joint and "Bamboo spine". Elevation of erythrocyte sedimentation rate in ankylosing spondylitis indicates active status of the disease. Authors have experienced 2 cases of anterior uveitis with Marie-Strumpell ankylosing spondylitis in our clinic. These cases have shown typical findings of "Bamboo spine", sacro iliac joint blurring and acute nongranulomatous unilateral uveal involvement.
Blood Sedimentation ; Great Britain ; HLA-B27 Antigen ; Humans ; Joints ; Male ; Sclerosis ; Spondylitis, Ankylosing* ; Uveitis ; Uveitis, Anterior*

BACKGROUND: The purpose of this study was to find out the effect of hypnotics and analgesics on oxygen saturation by pulse oximetry in surgery patients under spinal anesthesia. METHODS: Sixty-five patients classified ASA physical status 1 or 2 scheduled for surgery under spinal anesthesia were studied. These patients were divided into three groups. The 22 subjects of the first group did not receive either midazolam or fentanyl. The 22 subjects of the second group received midazolam. The 21 subjects of the third group received midazolam (0.03 mg/kg) and fentanyl (1.0 microgram/ kg). Oxygen saturation was measured with a pulse oximetry. Measurements were made before spinal anesthesia, 5 minutes, and 10 minutes after starting of spinal anesthesia, 5 minutes, 10 minutes, 30 minutes, and 60 minutes after the start of the operation or intravenous injection of drugs. In addition, measurements were made on arrival in the recovery room, and 5 minutes, 10 minutes, 20 minutes, and 30 minutes after arrival in the recovery room. RESULTS: There were statistically significant differences in oxygen saturation at 5 minutes after the start of the operation or intravenous injection of drugs. The mean oxygen saturation for the first group was 98.2 +/- 1.8%, for the second group 97.9 +/- 2.6%, and for the third group 92.4 +/- 2.8%. Hypoxia cases at 5 minutes after the start of the operation or intravenous injection of a drug occurred in 4.5% of the first group, 9.1% of the second group, and 57.1% of the third group CONCLUSIONS: We concluded that oxygen saturation monitoring should be done routinely in patients receiving hypnotics and analgesics during spinal anesthesia, and oxygen should be administered to patients who develope hypoxia during spinal anesthesia.
Analgesics* ; Anesthesia, Spinal* ; Anoxia* ; Fentanyl ; Humans ; Hypnotics and Sedatives* ; Incidence* ; Injections, Intravenous ; Midazolam ; Oximetry ; Oxygen ; Recovery Room

No abstract available.
Nevus*

The number of latex-induced allergic reactions, including anaphylaxis, has been increasing since latex induced contact urticaria was first reported in surgical patients by Nutter in 1979. Latex allergy is frequently seen in the healthcare industry, and is associated with systemic symptoms, hand eczema, and allergic contact dermatitis. Reactions range from contact urticaria, rhinitis and conjunctivitis, angioderma or bronchospasm to the recently recognized severe anaphylactic shock with cardiovascular collapse. We encountered a 55-year-old female patient who developed a severe anaphylactic reaction after manual vetilatory support using a facemask that contained latex. A subsequent allergy workup revealed a delayed-type hypersensitivity to latex. This case highlights the need for anesthesiologists to be able to diagnose the signs and symptoms of allergic reactions in patients during the peri-anesthetic period. Two types of allergic reactions to natural rubber latex (NRL) and rubber products are now known to exist: type I (immediate-type) and type IV (delayed-type hypersensitivity [DTH]). Patients with NRL allergy should be provided with information on non-latex devices and latex avoidance in medical, dental, and occupational settings. In addition, we should pay more attention to the management of allergic reactions to latex in high risk groups.
Anaphylaxis ; Bronchial Spasm ; Conjunctivitis ; Dermatitis, Allergic Contact ; Dermatitis, Contact ; Eczema ; Female ; Hand ; Health Care Sector ; Humans ; Hypersensitivity ; Latex ; Latex Hypersensitivity ; Masks ; Middle Aged ; Rhinitis ; Rubber ; Urticaria

Several studies have reported that heavy metals have a relation with osteoporosis and osteoporotic fractures. In this study, we investigated the association between heavy metal exposure status, as assessed by serum major heavy metals such as lead (Pb), cadmium (Cd), and mercury (Hg) and bone mineral density (BMD) status among Korean adults. A total of 64 adults participated in this study and were assigned to one of three study groups based on the T-score of spine BMD: a normal group (n = 21, T-score > -1), osteopenia group (n = 29, -2.5 < T-score < or = -1) and osteoporosis group (n = 14, T-score < or = -2.5). We also assessed serum levels of heavy metals (Pb, Cd and Hg) by using atomic absorption spectrometer (AAS) and daily nutrient and food intakes for 3 days by using 24-hr recall method in the subjects. The mean age was significantly higher in the osteoporosis group compared with the osteopenia and normal groups (p < 0.001). There was no significant difference in serum levels of Pb, Cd and Hg among the three groups after adjusting for age. Daily intakes of energy, nutrients were not significantly different among the three groups. The osteoporosis group consumed significantly higher fish and shellfish than the other groups (p < 0.05). In the correlation analysis controlling for age, sex, BMI, and BMD status, the serum Cd level was significantly negatively correlated with intake of cereals, milks and total food. In summary, we did not find a direct association between serum heavy metal levels and BMD status. However, negative relationships were found between serum heavy metal levels and intake of some foods.
Absorption ; Adult ; Bone Density ; Bone Diseases, Metabolic ; Cadmium ; Edible Grain ; Eating* ; Humans ; Metals, Heavy* ; Milk ; Osteoporosis ; Osteoporotic Fractures ; Shellfish ; Spine

Localized scleroderma is a connective tissue disorder characterized by thickening and fibrosis of the skin due to excessive collagen deposition in the dermis. Dystrophic calcinosis occurs when calcium is deposited in the previously damaged skin tissue, and calcium and phosphorous levels in the blood are within normal limits. It occurs secondary to connective tissue disease, infection, inflammatory processes, chronic venous stasis, cutaneous neoplasm, and trauma. We report a rare case of dystrophic calcinosis cutis with transepidermal elimination secondary to localized scleroderma.
Calcinosis ; Calcium ; Collagen ; Connective Tissue ; Connective Tissue Diseases ; Dermis ; Fibrosis ; Scleroderma, Localized ; Skin