1.A study on the cytotoxicity of the orthodontic bonding materials.
Korean Journal of Orthodontics 1992;22(1):147-158
This study was aimed to compare the relative cytotoxicity of the five common orthodontic bonding materials (Concise. Mon-lok , Ortho-One, Super C, Transbond) using cell culture technique. DNA synthesis of the fibroblasts was assessed by 3H-thymidine uptake to evaluate the effect of the bonding materials on the growth of the cells. The human gingival fibroblasts were explanted from the buccal gingiva of 10 year-old girl and cultured in a-MEM/10% FBS/1% antibiotics medium, 37degreesC, 5% CO2 incuvator. The gingival fibroblasts were tested with the medium into which the bonding materials had been soaked for 1 week. Or the bonding materials were placed on the cells immediately or 2 weeks after polymerization. After 22 hours, 3H-thymidine was added into the microtest wells and after 24 hours, the uptake of 3H-thymidine was determined by liquid scintilation counter. The results of this study were as follows. 1. DNA synthesis was significantly decreased with Super C and Transbond than Ortho-One, when treated with medium into which the bonding materials had been soaked for 1 week. 2. DNA synthesis was significantly decreased with Concise, Super C and Transbond than control, when treated immediately after polymerization. 3. DNA syntehsis was significantly decreased with Concise, Super C and Transbond than Ortho-One, when immediately after polymerization. 4. There was no significant difference in DNA synthesis between the bonding materials, when treated 2 weeks after polymerization.
Anti-Bacterial Agents
;
Cell Culture Techniques
;
Child
;
DNA
;
Female
;
Fibroblasts
;
Gingiva
;
Humans
;
Polymerization
;
Polymers
2.Musical murmur in a dog with acute chordae tendineae rupture.
Sa Hee MIN ; Min Hee KANG ; Hee Myung PARK
Korean Journal of Veterinary Research 2013;53(4):263-264
A 6 year-old, spayed female, Maltese dog was presented with precordial thrill and mild coughing. Thoracic auscultation revealed a grade V/VI systolic murmur with maximal intensity over the left apex characterized by musical murmur. Echocardiography revealed mild myxomatous degeneration of mitral valve and ruptured chordae tendineae. Musical murmur was produced due to the vibration of ruptured piece of chordae tendineae along with regurgitant flow. After treatment with furosemide and ramipril, clinical signs resolved and precordial thrill reduced. This case report describes typical clinical signs and phonocardiogram of musical murmur in a dog with acute chordae tendineae rupture.
Animals
;
Auscultation
;
Child
;
Chordae Tendineae*
;
Cough
;
Dogs*
;
Echocardiography
;
Female
;
Furosemide
;
Humans
;
Mitral Valve
;
Music*
;
Ramipril
;
Rupture*
;
Systolic Murmurs
;
Vibration
3.Comparision of remifentanil and remifentanil/midazolam for outpatient anesthesia in prolotherapy.
Hyung Suk LEE ; Dae Hyun JO ; Min Gu KIM ; Myung Hee KIM ; Sa Hyun PARK ; Sung Hee CHUNG
Korean Journal of Anesthesiology 2009;56(2):175-180
BACKGROUND: Prolotherapy is a therapeutic procedure used for chronic musculoskeletal and arthritic pain. It involves injecting an irritant solution to pain sites and causes patient discomfort, which can lead to treatment discontinuation. Remifentanil is an ultra short-acting micro-opiate receptor agonist that permits a rapid transition from intense analgesia to a minimal residual effect. Here, we evaluated the effect of remifentanil as a preparative medication for ambulatory prolotherapy. METHODS: Eighty patients taking prolotherapy were assigned into three groups for pre-therapeutic injections: remifentanil 0.1 microgram/kg/min alone (Group R), remifentanil 0.05 microgram/kg/min with midazolam 2 mg (Group M), and normal saline (Group C). Pain and sedation scores, blood pressure, pulse oxygen saturation, heart rate, satisfaction score, and time to discharge were measured. RESULTS: Pain scores in groups M and R were lower than group C during and after prolotherapy. The sedation score of group M was higher than groups R and C. Nine patients in group R experienced dizziness during prolotherapy. In group M, 8 patients experienced dizziness and 2 patients experienced nausea. There was no difference in time to discharge among all groups. Satisfaction scores in group M (7.3 +/- 0.8) and group R (7.0 +/- 0.8) were higher than that of group C (5.3 +/- 0.6). CONCLUSIONS: Remifentanil and remifentanil/midazolam effectively reduce the pain produced by prolotherapy.
Analgesia
;
Anesthesia
;
Blood Pressure
;
Dizziness
;
Heart Rate
;
Humans
;
Midazolam
;
Nausea
;
Outpatients
;
Oxygen
;
Piperidines
4.Characteristic Clinical Features and Survival in Cats with Symptomatic Hypertrophic Cardiomyopathy.
Min Hee KANG ; Sa Hee MIN ; Seung Gon KIM ; Chang Min LEE ; Hee Myung PARK
Journal of Biomedical Research 2015;16(4):152-158
Hypertrophic cardiomyopathy (HCM) is recognized as the most common feline cardiac disease. Several studies have evaluated the population characteristics and survival time of cats with HCM; however, these reports included large numbers of asymptomatic HCM. The purpose of this study was to evaluate the characteristics and survival time of cats with symptomatic HCM admitted to emergency service. Medical records were examined to verify clinical diagnosis of HCM. Asymptomatic cats diagnosed with HCM were also excluded from the study. From a total of 13 cats, eight were classified in the arterial thromboembolism (ATE) group while the other five were in the congestive heart failure (CHF) group. Middle-aged (6 years, range 1.4~7 years) male cats (53.8%) were included in this study. Pelvic limb paralysis, depression, and respiratory distress were common clinical signs found in symptomatic HCM cats. Hematologic evaluation found that enzymes related to muscle damage and tissue necrosis were elevated in both groups and highly elevated in the ATE group. Left atrium was remarkably enlarged in ATE group cats. The median survival time of cats in the ATE group was significantly shorter than that of cats in the CHF group (P=0.002). Prospective investigation based on a large population would be required to clarify the effects of various factors on prognosis of HCM cats.
Animals
;
Cardiomyopathy, Hypertrophic*
;
Cats*
;
Depression
;
Diagnosis
;
Emergencies
;
Extremities
;
Heart Atria
;
Heart Diseases
;
Heart Failure
;
Humans
;
Male
;
Medical Records
;
Necrosis
;
Paralysis
;
Population Characteristics
;
Prognosis
;
Prospective Studies
;
Thromboembolism
5.Secondary Esophageal Reconstruction for Esophageal Atresia.
Young Jo SA ; Jae Kil PARK ; Hae Myung JEON ; Young Kyu MOON ; Sung Bo SIM ; Sun Hee LEE
The Korean Journal of Thoracic and Cardiovascular Surgery 2008;41(5):671-674
Jejunum and fasciocutaneous and myocutaneous flaps are theoretically recommended as esophageal substitutes in reconstruction of the esophagus after several occurrences of failed reconstruction. However, other esophageal substitutes should also be considered. Secondary esophageal reconstruction was successfully performed on a 24-year- old woman who had a history of cervical, thoracic, and abdominal operations and esophageal stricture secondary to several failed reconstructions for esophageal atresia and tracheo-esophageal fistula 21 years prior. The esophageal reconstruction was done subcutaneously by end-to-side anastomosis at the left cervical area using a deformed stomach graft. The patient was discharged with the ability to consume a regular diet after the operation.
Diet
;
Esophageal Atresia
;
Esophageal Stenosis
;
Esophagus
;
Female
;
Fistula
;
Humans
;
Reoperation
;
Stomach
;
Transplants
6.Three Cases of High Signal Intensity by Brain Magnetic Resonance Imaging in CO2 arc Welders.
Young Seoub HONG ; Myung Ah LIM ; Yong Hee LEE ; Hae Kwan CHEONG ; Ji Yong KIM ; Hyun Sul LIM ; Jung Jeung LEE ; Kong Joon SA ; Joon Youn KIM
Korean Journal of Occupational and Environmental Medicine 1998;10(2):290-298
We experienced three cases of high signal intensity observed by Brain Magnetic Resonance Imaging in CO2 arc welders of steel-frame manufacturing industry. Case 1 was a 35 years old man who has been an CO2 arc welder for 10 years, admitted a sudden onset of tonic clonic seizure. He complained fever, chilling and myalgia since 3 days before admission. On admission, in the test of manganese exposure indices, manganese concentrations of blood, urine and scalp hair were 5.17 microgram/dL, 22.00 microgram/l and 31.25 ppm respectively. Case 2 was a 35 years old man who has been an CO2 arc welder for 20 years. On admission, He complained fatigue, numbness and weakness of extremities, and decrease of libido. In the test of manganese exposure indices, manganese concentrations of blood, urine and scalp hair were 6.34 microgram/dL, 14.62 microgram/l and 57.87 ppm respectively. In neurologic examination, Palmo-mentis reflex and Myerson sign were observed. Case 3 was a 33 years old man who has been an CO2 arc welder for 16 years. On admission, He complainer loss of appetite, numbness of extremities, fatigue and decrease of attention. In the test of manganese exposure indices, manganese concentrations of blood, urine and scalp hair were 5.14 microgram/dL, 13.79 microgram/l and 50.08 ppm respectively. In neurologic examination, Myerson sign was observed. In brain magnetic resonance imaging, T1WI showed symmetrical high signal intensity in basal ganglia and midbrain of three cases. Authors argued that they were developed by manganese exposure, and we considered that follow up study would be necessary.
Adult
;
Appetite
;
Basal Ganglia
;
Brain*
;
Extremities
;
Fatigue
;
Fever
;
Follow-Up Studies
;
Hair
;
Humans
;
Hypesthesia
;
Libido
;
Magnetic Resonance Imaging*
;
Manganese
;
Mesencephalon
;
Myalgia
;
Neurologic Examination
;
Reflex
;
Scalp
;
Seizures
7.Facial dysmorphisms associated with holoprosencephaly.
Min Jeong KIM ; In Yang PARK ; Yon Hee KIM ; Mi Na CHOI ; Myung Shin KIM ; Young LEE ; Jong Chul SHIN ; Sa Jin KIM
Korean Journal of Obstetrics and Gynecology 2008;51(4):399-406
OBJECTIVE: The purpose of this study is to determine and classify holoprosencephlay with the associated facial abnormalities. METHODS: This was a retrospective study in which were reviewed the sonographic findings in correlation with the clinical and pathologic data of fetuses or neonates identified with holoprosencephaly at the Department of Obstetrics and Gynecology of the Catholic University in the period 1995-2007. RESULTS: Twelve cases with a Holoprosencephaly were found. Mean gestational age at diagnosis and delivery was 28 weeks of pregnancy (range from 14 to 41 weeks). Modes of delivery were 8 cases of preterm spontaneous delivery, 2 cases of missed abortion, 1 case of normal fullterm spontaneous delivery, and 1 case of full term cesarean delivery. Associated facial anomalies were present in 9/12 cases (75%) which involved with cyclopia, proboscis, cleft lip and palate, ethomocephaly and otocephaly. Among those anomalies, abnormal karyotypes were 3/6 cases (50%). CONCLUSIONS: When a midline brain anomaly is detected by antenatal sonography, accurate sonographic analysis of midline facial defect may allow more definitive diagnosis of holoprosencephaly, and the outcome of affected fetuses often have other major structural abnormalities and nearly always fatal.
Abnormal Karyotype
;
Abortion, Missed
;
Brain
;
Cleft Lip
;
Craniofacial Abnormalities
;
Female
;
Fetus
;
Gestational Age
;
Gynecology
;
Holoprosencephaly
;
Humans
;
Infant, Newborn
;
Obstetrics
;
Palate
;
Pregnancy
;
Retrospective Studies
8.A multicenter cross-sectional study on the prevalence of overactive bladder and its impacts on the quality of life in gynecologic outpatients
Do Young KIM ; Da Hae JANG ; Hyun Jin KIM ; Sa Ra LEE ; Myung Jae JEON ; Jung Ho SHIN ; Hee Dong CHAE
Obstetrics & Gynecology Science 2020;63(2):181-186
OBJECTIVE: The present study assessed the prevalence of overactive bladder (OAB) symptoms in gynecologic outpatients and the effects of OAB on a patient's quality of life.METHODS: The survey was completed by adult female patients who visited the gynecology clinic for examination or treatment of gynecologic conditions. The subjects who met the exclusion criteria were excluded. The survey included a questionnaire assessing general characteristics, the purpose of the visit, diagnosis, and the presence or absence of OAB. The subjects were asked whether they were under medical treatment for OAB symptoms, and the degree of discomfort in daily activities was evaluated.RESULTS: In total, 1,990 female subjects visiting the gynecology clinics of 4 different institutions were recruited. On OAB Symptom Score, 188 subjects responded with scores of ≥2 for question #3 (≥1 time(s) weekly). The severity of OAB symptoms was as follows: 345 subjects had mild OAB, 127 subjects had moderate OAB, and 12 subjects had severe OAB. The prevalence of OAB symptoms was 10.30%.CONCLUSION: The present study confirmed that OAB symptoms are common, with a prevalence of 10.30%. Patients with OAB symptoms frequently neglect to seek medical intervention. Our findings will help inform patients to recognize their condition and seek medical assistance, especially in gynecologic outpatient clinics.
9.A Case-Control Study to Identify Risk Factors for Totally Implantable Central Venous Port-Related Bloodstream Infection.
Guk Jin LEE ; Sook Hee HONG ; Sang Young ROH ; Sa Rah PARK ; Myung Ah LEE ; Hoo Geun CHUN ; Young Seon HONG ; Jin Hyoung KANG ; Sang Il KIM ; Youn Jeong KIM ; Ho Jong CHUN ; Jung Suk OH
Cancer Research and Treatment 2014;46(3):250-260
PURPOSE: To date, the risk factors for central venous port-related bloodstream infection (CVP-BSI) in solid cancer patients have not been fully elucidated. We conducted this study in order to determine the risk factors for CVP-BSI in patients with solid cancer. MATERIALS AND METHODS: A total of 1,642 patients with solid cancer received an implantable central venous port for delivery of chemotherapy between October 2008 and December 2011 in a single center. CVP-BSI was diagnosed in 66 patients (4%). We selected a control group of 130 patients, who were individually matched with respect to age, sex, and catheter insertion time. RESULTS: CVP-BSI occurred most frequently between September and November (37.9%). The most common pathogen was gram-positive cocci (n=35, 53.0%), followed by fungus (n=14, 21.2%). Multivariate analysis identified monthly catheter-stay as a risk factor for CVP-BSI (p=0.000), however, its risk was lower in primary gastrointestinal cancer than in other cancer (p=0.002). Initial metastatic disease and long catheter-stay were statistically significant factors affecting catheter life span (p=0.005 and p=0.000). Results of multivariate analysis showed that recent transfusion was a risk factor for mortality in patients with CVP-BSI (p=0.047). CONCLUSION: In analysis of the results with respect to risk factors, prolonged catheter-stay should be avoided as much as possible. It is necessary to be cautious of CVP-BSI in metastatic solid cancer, especially non-gastrointestinal cancer. In addition, avoidance of unnecessary transfusion is essential in order to reduce the mortality of CVP-BSI. Finally, considering the fact that confounding factors may have affected the results, conduct of a well-designed prospective controlled study is warranted.
Case-Control Studies*
;
Catheter-Related Infections
;
Catheters
;
Drug Therapy
;
Fungi
;
Gastrointestinal Neoplasms
;
Gram-Positive Cocci
;
Humans
;
Mortality
;
Multivariate Analysis
;
Risk Factors*
10.Genomic Diversity of Helicobacter pylori.
Won Kon LEE ; Sang Haeng CHOI ; Seon Gyu PARK ; Yeo Jeong CHOI ; Mi Young CHOE ; Jeong Won PARK ; Sun Ae JUNG ; Eun Young BYUN ; Jae Young SONG ; Tae Sung JUNG ; Byung Sang LEE ; Seung Chul BAIK ; Myung Je CHO ; Hee Shang YOUN ; Gyung Hyuck KO ; Yong Sung KIM ; Jong Hoon PARK ; Dae Sil LEE ; Hyang Sook YOO ; Sa Youl GHIM ; Kwang Ho LEE
Journal of the Korean Society for Microbiology 1999;34(6):519-532
Helicobacter pylori is a causative agent of type B gastritis and plays a central role in the pathogenesis of gastroduodenal ulcer and gastric cancer. To elucidate the host-parasite relationship of the H. pylori infection on the basis of molecular biology, we tried to evaluate the genomic diversity of H. pylori. An ordered overlapping bacterial artificial chromosome (BAC) library of a Korean isolate, H, pylori 51 was constructed to set up a genomic map. A circular physical map was constructed by aligning ApaI, Notl and SfiI-digested chromosomal DNA. When the physical map of H. pylori 51 was compared to that of unrelated strain, H. pylori 26695, completely different restriction patterns were shown. Fifteen known genes were mapped on the chromosome of H. pylori 51 and the genetic map was compared with those of strain 26695 and J99, of which the entire genomic sequences were reported. There were some variability in the gene location as well as gene order among three strains. For further analysis on the genomic diversity of H. pylori, when comparing the genomic structure of 150 H. pylori Korean isolates with one another, genomic macrodiversity of H. pylori was characterized by several features: whether or not susceptible to restriction digestion of the chromsome, variation in chromosomal restriction fingerprint and/or high frequency of gene rearrangement. We also examined the extent of allelic variation in nucleotide or deduced amino acid sequences at the individual gene level. fucT, cagA and vacA were confirmed to carry regions of high variation in nucleotide sequence among strains. The plasticity zone and strain-specific genes of H. pylori 51 were analyzed and compared with the former two genomic sequences. It should be noted that the H. pylori 51-specific sequences were dispersed on the chromosome, not congregated in the plasticity zone unlike J99- or 26695-specific genes, suggesting the high frequency of gene rearrangement in H. pylori genome. The genomc of H. pylori 51 shows differences in the overall genomic organization, gene order, and even in the nucleotide sequences among the H. pylori strains, which are far greater than the differences reported on the genomic. diversity of H. pylori.
Amino Acid Sequence
;
Base Sequence
;
Chromosomes, Artificial, Bacterial
;
Dermatoglyphics
;
Digestion
;
DNA
;
Gastritis
;
Gene Order
;
Gene Rearrangement
;
Genome
;
Helicobacter pylori*
;
Helicobacter*
;
Host-Parasite Interactions
;
Molecular Biology
;
Peptic Ulcer
;
Plastics
;
Stomach Neoplasms