1.Torsion of a mucocele of the vermiform appendix: a case report and review of the literature.
Chang Ho LEE ; Min Ro LEE ; Jae Chun KIM ; Myoung Jae KANG ; Yeon Jun JEONG
Journal of the Korean Surgical Society 2011;81(Suppl 1):S47-S50
Torsion of a mucocele of the vermiform appendix is an extremely rare condition and also a rare cause of an acute abdomen with a clinical presentation that is indistinguishable from acute appendicitis, and thus, the condition is diagnosed during operation. Here, the authors describe the case of a 78-year-old female, who presented with intermittent abdominal pain. The appendix had a pelvic position and the torsion was counterclockwise. In addition, the torsion was associated with mucocele of the appendix, which was considered a secondary factor of torsion. Appendectomy and drainage were performed.
Abdomen, Acute
;
Abdominal Pain
;
Aged
;
Appendectomy
;
Appendicitis
;
Appendix
;
Drainage
;
Female
;
Humans
;
Mucocele
;
Torsion Abnormality
2.A Case of Severe Pituitary Dwarfism due to Agenesis of Anterior Pituitary Gland with Pituitary Stalk Transection.
Myoung Ju YOO ; Dong Ki HAN ; Jeh Hoon SHIN ; In Jun SEUL ; Seung Ro LEE
Journal of the Korean Pediatric Society 1994;37(9):1305-1311
We experienced one case of severe pituitary dwarfism in a 10 years old female girl. Magnetic resonance image (MRI) revealed transection of the pituitary stalk stalk with the formation of high intensity ectopic posterior lobe located at the median eminence and agenesis of an anterior lobe of pituitary gland. The serum growth Hormone (GH) response to clonidine and L-dopa revealed severe GH deficiency. The patient had responses to TRH, normal TSH and partial prolactin response, respectively. There was not response LH and FSH to GnRH. The morning cortisol concentration and serum T4 concentration were decreased below the normal range. These findings and no hyperprolactinemia suggested the presence of a vascular connection between the pituitary gland and hypothalamus, which is not visible on MRI. Sofar, the primary cause of idiopathic pituitary dwarfism in many patients is injury to hypothalamus by perinatal insults. In this patient, there was no history of perinatal insults and postnatal head trauma but transection of the pituitary stalk. We report a case of severe pituitary dwarfism due to agenesis with brief review of related litereature.
Child
;
Clonidine
;
Craniocerebral Trauma
;
Dwarfism, Pituitary*
;
Female
;
Gonadotropin-Releasing Hormone
;
Growth Hormone
;
Humans
;
Hydrocortisone
;
Hyperprolactinemia
;
Hypothalamus
;
Levodopa
;
Magnetic Resonance Imaging
;
Median Eminence
;
Pituitary Gland*
;
Pituitary Gland, Anterior*
;
Prolactin
;
Reference Values
3.Parentage test using HLA and red cell antigen systems.
Myoung Hee PARK ; Kyou Sup HAN ; Jung Bin LEE ; Hyung Ro MOON
Korean Journal of Clinical Pathology 1992;12(1):85-91
No abstract available.
4.Adenoid Basal Cell Tumor of the Prostate: A case report.
Joo Heon KIM ; Woo Sung MOON ; Myoung Jae KANG ; Dong Geun LEE ; Jae Y RO
Korean Journal of Pathology 2000;34(7):534-536
Adenoid basal cell tumor of the prostate is a rare tumorous lesion that can be misdiagnosed as adenocarcinoma of the prostate. The malignant potential of adenoid basal cell tumor remains uncertain due to small number of reported cases. This 66-year-old man presented with symptoms of urinary tract obstruction. Under the impression of benign prostatic hyperplasia, a transurethral resection of the prostate (TURP) was performed. The patient was alive with no evidence of recurrence or metastasis 15 months after TURP. Microscopically, most of the lesions were composed of nodular collections of small nests of basaloid cells with peripheral palisading, and clusters of tumor cells forming cribriform pattern. Multiple areas of basal cell hyperplasia and atypical basal cell hyperpalsia were also observed. The coexistence of basal cell hyperplasia, atypical basal cell hyperpalsia, and adenoid basal cell tumor with cribriform pattern in this case supports a morphologic continuum from the benign hyperplastic lesion to malignant neoplasia.
Adenocarcinoma
;
Adenoids*
;
Aged
;
Humans
;
Hyperplasia
;
Neoplasm Metastasis
;
Prostate*
;
Prostatic Hyperplasia
;
Recurrence
;
Transurethral Resection of Prostate
;
Urinary Tract
5.Parentage test using HLA and red cell antigen systems.
Myoung Hee PARK ; Kyou Sup HAN ; Jung Bin LEE ; Hyung Ro MOON
Korean Journal of Clinical Pathology 1993;13(1):85-91
No abstract available.
6.Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013–2016
Da Won MA ; Myoung Ro LEE ; Sung Hee HONG ; Shin Hyeong CHO ; Sang Eun LEE
The Korean Journal of Parasitology 2019;57(5):531-536
Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013–2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and β-giardin genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the β-giardin gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for continuous monitoring of these zoonotic pathogens and provide a basis for implementing control and prevention strategies. Further, the results might be useful for epidemiological investigation of the source of outbreak.
Child
;
Cryptosporidium parvum
;
Cryptosporidium
;
Diarrhea
;
Genotype
;
Giardia lamblia
;
Giardia
;
Glycoproteins
;
Humans
;
Korea
;
Molecular Epidemiology
;
Polymerase Chain Reaction
;
Prevalence
7.Outbreak of Cyclosporiasis in Korean Travelers Returning from Nepal
Da-Won MA ; Myoung-Ro LEE ; Bora KU ; Shin-Hyeong CHO ; Sang-Eun LEE
The Korean Journal of Parasitology 2020;58(5):589-592
Cyclospora cayetanensis is an apicomplexan protozoan and is one of the most common pathogens causing chronic diarrhea worldwide. Eight stool samples with diarrheal symptom out of 18 Korean residents who traveled to Nepal were obtained, and examined for 25 enteropathogens including 16 bacterial species, 5 viral species, and 4 protozoans in stool samples as causative agents of water-borne and food-borne disease. Only C. cayetanensis was detected by nested PCR, and 3 PCR-positive samples were sequenced to confirm species identification. However, the oocysts of C. cayetanensis in fecal samples could not be detected by direct microscopy of the stained sample. As far as we know, this is the first report of a group infection with C. cayetanensis from a traveler visiting Nepal, and the second report of a traveler’s diarrhea by C. cayetanensis imported in Korea.
8.Antitumor Effect of Low-Dose of Rapamycin in a Transgenic Mouse Model of Liver Cancer
Hyung Soon LEE ; Joon Ye KIM ; Simon Weonsang RO ; Myoung Soo KIM ; Haeryoung KIM ; Dong Jin JOO
The Korean Journal of Gastroenterology 2022;63(11):1007-1015
Purpose:
We investigate whether low-dose rapamycin is effective in preventing hepatocellular carcinoma (HCC) growth and treating HCC after tumor development in transgenic mice.
Materials and Methods:
We established transgenic mice with HCC induced by activated HrasG12V and p53 suppression. Transgenic mice were randomly assigned to five experimental groups: negative control, positive control, tacrolimus only, rapamycin only, and tacrolimus plus rapamycin. The mice were further divided into two groups according to time to commencement of immunosuppressant treatment: de novo treatment and post-tumor development.
Results:
In the de novo treatment group, marked suppression of tumor growth was observed in the rapamycin only group. In the post-tumor development group, the rapamycin only group displayed no significant suppression of tumor growth, compared to the positive control group. In T lymphocyte subset analysis, the numbers of CD4+ effector T cells and CD4+ regulatory T cells were significantly lower in the positive control, tacrolimus only, and tacrolimus plus rapamycin groups than the negative control group.Immunohistochemical analysis revealed significantly higher expression of phosphorylated-mTOR, 4E-BP1, and S6K1 in the positive control group than in the rapamycin only group.
Conclusion
Low-dose rapamycin might be effective to prevent HCC growth, but may be ineffective as a treatment option after HCC development.
9.Antitumor Effect of Low-Dose of Rapamycin in a Transgenic Mouse Model of Liver Cancer
Hyung Soon LEE ; Joon Ye KIM ; Simon Weonsang RO ; Myoung Soo KIM ; Haeryoung KIM ; Dong Jin JOO
Yonsei Medical Journal 2022;63(11):1007-1015
Purpose:
We investigate whether low-dose rapamycin is effective in preventing hepatocellular carcinoma (HCC) growth and treating HCC after tumor development in transgenic mice.
Materials and Methods:
We established transgenic mice with HCC induced by activated HrasG12V and p53 suppression. Transgenic mice were randomly assigned to five experimental groups: negative control, positive control, tacrolimus only, rapamycin only, and tacrolimus plus rapamycin. The mice were further divided into two groups according to time to commencement of immunosuppressant treatment: de novo treatment and post-tumor development.
Results:
In the de novo treatment group, marked suppression of tumor growth was observed in the rapamycin only group. In the post-tumor development group, the rapamycin only group displayed no significant suppression of tumor growth, compared to the positive control group. In T lymphocyte subset analysis, the numbers of CD4+ effector T cells and CD4+ regulatory T cells were significantly lower in the positive control, tacrolimus only, and tacrolimus plus rapamycin groups than the negative control group.Immunohistochemical analysis revealed significantly higher expression of phosphorylated-mTOR, 4E-BP1, and S6K1 in the positive control group than in the rapamycin only group.
Conclusion
Low-dose rapamycin might be effective to prevent HCC growth, but may be ineffective as a treatment option after HCC development.
10.Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide.
Ah Young LEE ; Ji Myung CHOI ; Myoung Hee LEE ; Jaemin LEE ; Sanghyun LEE ; Eun Ju CHO
Nutrition Research and Practice 2018;12(2):93-100
BACKGROUND/OBJECTIVE: Oxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide (H₂O₂)-induced oxidative stress in SH-SY5Y neuronal cells. MATERIALS/METHODS: The SH-SY5Y human neuroblastoma cells exposed to 250 µM H₂O₂ for 24 h were treated with different concentrations of PO (25, 125, 250 and 500 µg/mL) and its major fatty acid, ALA (1, 2.5, 5 and 25 µ/mL). We examined the effects of PO and ALA on H₂O₂-induced cell viability, lactate dehydrogenase (LDH) release, and nuclear condensation. Moreover, we determined whether PO and ALA regulated the apoptosis-related protein expressions, such as cleaved-poly ADP ribose polymerase (PARP), cleaved caspase-9 and -3, BCL-2 and BAX. RESULTS: Treatment of H₂O₂ resulted in decreased cell viability, increased LDH release, and increase in the nuclei condensation as indicated by Hoechst 33342 staining. However, PO and ALA treatment significantly attenuated the neuronal cell death, indicating that PO and ALA potently blocked the H₂O₂-induced neuronal apoptosis. Furthermore, cleaved-PARP, cleaved caspase-9 and -3 activations were significantly decreased in the presence of PO and ALA, and the H₂O₂-mediated up-regulated BAX/BCL-2 ratio was blocked after treatment with PO and ALA. CONCLUSIONS: PO and its main fatty acid, ALA, exerted the protective activity from neuronal oxidative stress induced by H₂O₂. They regulated apoptotic pathway in neuronal cell death by alleviation of BAX/BCL-2 ratio, and down-regulation of cleaved-PARP and cleaved caspase-9 and -3. Although further studies are required to verify the protective mechanisms of PO and ALA from neuronal damage, PO and ALA are the promising agent against oxidative stress-induced apoptotic neuronal cell death.
Adenosine Diphosphate Ribose
;
alpha-Linolenic Acid*
;
Apoptosis
;
Caspase 9
;
Cell Death*
;
Cell Survival
;
Down-Regulation
;
Humans
;
Hydrogen Peroxide*
;
Hydrogen*
;
L-Lactate Dehydrogenase
;
Neuroblastoma
;
Neurodegenerative Diseases
;
Neurons*
;
Neuroprotective Agents
;
Oxidative Stress
;
Perilla*