The p53 protein was discovered in 1979 as cellular 53-kD nuclear phosphoprotein bound to the large transforming antigen of SV40 virus. P21WAF1/CIP1, which has been described as the critical downstream mediator of p53, is known to suppress DNA replication and arrest the G1 cell cycle by quaternary complex with cyclin D, cyclin-dependent kinase(CDK) and proliferating cell nuclear antigen(PCNA). In these days, some studies shows that the p21 can be induced by independent pathways. There are various reports about the expression of p21 (67%.82.4%) in oral squamous cell carcinoma. But these studies are mostly done in malignant tumor not in benign tumor. So we decided to study the expression of p21 in ameloblastoma and the relationship between p53 and p21 as a downstream mediator of p53 in ameloblastoma. We investigated the expression of p21 and p53 with the method of immunohistochemistry. We selected 30 cases of ameloblastoma tissue blocks (acanthomatous type: 5 cases, follicular type: 8 cases, plexiform type: 17 cases) imbedded in paraffin. We used 30 cases of normal gingival tissues and 30 cases of squamous cell carcinoma tissues (SCC) respectively and compared their results with those of ameloblastoma. We made slides with the streptavidin-biotin methods and used monoclonal antibody DO-7 (Novocastra, Newcastle, United Kingdom) as p53 antibody and monoclonal antibody M7202 (DAKO, California, U.S.A.) as p21 antibody. We used Pearson's correlation coefficient to analyse the relationship. The results were as follows: 1. p21 was expressed in ameloblastoma about 30% and this is lower than that of normal gingiva and SCC. 2. In normal gingiva and ameloblastoma, p21 expression was correlated with p53 expression. 3. In SCC, p21 were expressed about 83.3% and this is more than that of p53. But there was no correlation between p21 and p53 expression. We confirmed p21 expression and relation with p53 in ameloblastoma. But, to confirm the function of p21, more studies about p21 expression in malignant ameloblastoma and ameloblastic carcinoma are needed.
Ameloblastoma* ; Ameloblasts ; California ; Carcinoma, Squamous Cell ; Cell Cycle ; Cyclin D ; DNA Replication ; Gingiva ; Immunohistochemistry ; Paraffin ; Simian virus 40

No abstract available.
Aorta, Thoracic*

We experienced one case of severe pituitary dwarfism in a 10 years old female girl. Magnetic resonance image (MRI) revealed transection of the pituitary stalk stalk with the formation of high intensity ectopic posterior lobe located at the median eminence and agenesis of an anterior lobe of pituitary gland. The serum growth Hormone (GH) response to clonidine and L-dopa revealed severe GH deficiency. The patient had responses to TRH, normal TSH and partial prolactin response, respectively. There was not response LH and FSH to GnRH. The morning cortisol concentration and serum T4 concentration were decreased below the normal range. These findings and no hyperprolactinemia suggested the presence of a vascular connection between the pituitary gland and hypothalamus, which is not visible on MRI. Sofar, the primary cause of idiopathic pituitary dwarfism in many patients is injury to hypothalamus by perinatal insults. In this patient, there was no history of perinatal insults and postnatal head trauma but transection of the pituitary stalk. We report a case of severe pituitary dwarfism due to agenesis with brief review of related litereature.
Child ; Clonidine ; Craniocerebral Trauma ; Dwarfism, Pituitary* ; Female ; Gonadotropin-Releasing Hormone ; Growth Hormone ; Humans ; Hydrocortisone ; Hyperprolactinemia ; Hypothalamus ; Levodopa ; Magnetic Resonance Imaging ; Median Eminence ; Pituitary Gland* ; Pituitary Gland, Anterior* ; Prolactin ; Reference Values

No abstract available.
Neoplasm Metastasis* ; Pancreatic Neoplasms* ; Scalp* ; Thorax*

No abstract available.
Child* ; Humans ; Syringoma* ; Vulva

This paper present 105 cases which were admitted to the Department of Pediatrics of Dong San Presbyterian Hospital from Jan. 1978 to July, 1980 The results are summarized as follows; 1) Age incidence, 94% of patients were under age of two, 88.2% of patients were under one year, and adults were high incidence in Korea. 2) Male to female ratio was 2.1:1 3) In seasonal incidence, spring was relatively high. 4) In 76.4% of cases, their body weight were over 50 percentile of Korean normal infants and children. 5) The patients who visited hospital within 24 hrs. from onset of symptom was occupied in 57.7%. 6) The cardinal symptom and signs were vomiting (89.4%), abdominal pain. Irritability, crying, (72.9%), mucous bloody stool (70.6%) and abdominal mass (42.1%) 7) In analysis by intussusception type(40.0%), ileocolic type (23.5%), ileoileal type(17.6%), jejunojejunal type (17.6%), in adults. 8) In simple abdominal X-ray figures showed the dilatation due to gastrostomy 52.9% and phenomenon of fluid retention 30.6%. 9) Etiologic causes were confirmed only 7.1% in children and 29.4% in adults, the causes in 92% were idiopathic in children and 70.6% in adults respectively. 10) In previous illness, we find out its predisposing factors 42.4% in children. 11) Hydrostatic barium reduction was successed in 47.1% of all cases and operative cases were 47%
Abdominal Pain ; Adult ; Barium ; Body Weight ; Causality ; Child ; Crying ; Dilatation ; Female ; Gastrostomy ; Humans ; Incidence ; Infant ; Intussusception* ; Korea ; Male ; Pediatrics ; Protestantism ; Seasons ; Vomiting

No abstract available.
Rhytidoplasty*

BACKGROUND: Recently, measurement of heart rate variability and the nonlinear complexity of heart rate dynamics have been used as indicators of cardiovascular health. Hypertensive patients showed alternation of cardiovascular homeostasis. We designed this study to evaluate the effect of anesthesia in hypertensive patients with approximate entropy (ApEn), representing the nonlinear complexity. METHODS: With informed consent, none premedicated normotensive (n=18) and hypertensive patients (n=18) were included in this study. ECG data were collected from 10 minutes before induction to 15 minutes after induction. Collected ECG data were stored into computer binary files. We calculated ApEn from the collected ECG data. RESULTS: Before induction, ApEn of hypertensive patients was significantly lower than that of normotensive patients (p<0.05). During induction and maintenance of anesthesia, there was no difference of ApEn between the two groups. ApEn of normotensive patients during induction and maintenance of anesthesia was significantly lower than that of pre-induction (p<0.05). ApEn during maintenance of anesthesia was lower than that of induction of anesthesia (p<0.05). ApEn of hypertensive group during maintenace of anesthesia was significantly lower than that of pre-induction of anesthesia (p<0.05). CONCLUSIONS: As the ApEn of hypertensive patients is lower than that of normotensive patients during pre-inducton period, the heart rate dynamics of hypertensive patients is more regular normotensive patients. The anesthesia is deepened, the heart rate dynamics of the both group is more regular. During the maintenance of anesthesia, the regularity of the heart rate dynamics that not different in both group from the results.
Anesthesia* ; Electrocardiography ; Entropy* ; Heart Rate ; Homeostasis ; Humans ; Hypertension* ; Informed Consent

No abstract available.
Hand* ; Skin* ; Transplants*

PURPOSE: To compare the Hong's grading method, utilized for slit-lamp grading of the mid-peripheral angle, with other conventional grading methods. Actual angle was measured by ultrasound biomicroscopy (UBM) for comparison. METHODS: Fifteen eyes with narrow angles and eighteen eyes with wide angles were studied. The average age of thirty-three subjects was 46.8+/-19.2, and the male to female ratio was 1:3.7. The mid-peripheral angle was graded by Hong's, van Herick's and Spaeth's grading system. The actual anterior chamber angle was evaluated by UBM and the correlation between each grading system and actual angle was investigated. RESULTS: The results from the Hong's grading (r=0.802, R2=0.643, p<0.01), Spaeth's grading (r=0.728, R2=0.530, p<0.01) and van Herick's grading (r=0.618, R2=0.382, p<0.01) methods correlated significantly with the actual angles. CONCLUSIONS: The Hong's grading method was an easy and effective tool to evaluate anterior chamber angle. In addition, the results of the Hong's grading method were closer to the actual angle than the other methods.
Anterior Chamber ; Female ; Glaucoma ; Humans ; Male ; Microscopy, Acoustic