1.Two Cases of Septic Arthritis by Mycoplasma hominis after Total Knee Replacement Arthroplasty.
Ji Hyun LEE ; Jang Ho LEE ; Nam Yong LEE ; Chul Won HA ; Doo Ryeon CHUNG ; Kyong Ran PECK
The Korean Journal of Laboratory Medicine 2009;29(2):135-139
Mycoplasma hominis has been related with pelvic inflammatory illnesses and postpartum and neonatal infections. Extragenital M. hominis infections are rare, but septicemia, septic arthritis, wound infection, meningitis, and other infections in Immunocompromised patients have also been described. Here we report two cases of septic arthritis caused by M. hominis in patients following total knee replacement arthroplasty. After the surgery, the patients presented with knee pain and clinical signs of infection, such as fever, erythema and swelling on the surgical site. Arthroscopic debridement operations were performed on the surgical site. M. hominis was isolated from the joint fluid and identified by the microscopic visualization of the typical "fried-egg-type" colonies on Mycoplasma specific agar (pleuropneumonia-like organism agar). It was also confirmed by 16S rRNA sequencing. To the best of our knowledge, this is the first report of prosthetic joint infections with M. hominis in Korea.
Aged
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Arthritis, Infectious/*diagnosis/etiology/microbiology
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*Arthroplasty, Replacement, Knee
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Humans
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*Knee Joint
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Male
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Mycoplasma Infections/*diagnosis/etiology/microbiology
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*Mycoplasma hominis
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RNA, Ribosomal, 16S/analysis/genetics
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Sequence Analysis, RNA
2.Analysis of non-bacterial respiratory pathogen infection in children with asthmatic diseases.
Chang-Zhen LI ; Jing-Jing RAO ; Rong WANG ; Hong SUN ; Hong-Wu AI
Chinese Journal of Contemporary Pediatrics 2012;14(11):834-837
OBJECTIVETo investigate the association of non-bacterial respiratory pathogens with asthmatic diseases in children, and the clinical significance of total serum IgE levels and peripheral eosinophil count in infection with non-bacterial respiratory pathogens.
METHODSIndirect immunofluorescence assay was used to detect IgM antibodies against nine types of non-bacterial respiratory pathogens in the sera of 490 children with asthmatic diseases between September 2010 and September 2011. Pathogens were analyzed and total serum IgE levels and peripheral eosinophil count were measured in IgM-positive cases.
RESULTSOf the 490 children with asthmatic diseases, 47.6% (233 cases) were positive with IgM antibodies against non-bacterial respiratory pathogens, the most common being Mycoplasma pneumoniae (MP) (25.3%), followed by adenovirus (ADV) (8.9%) and influenza B virus (Flu B) (8.8%). Thirty-six cases suffered from co-infection of two or more non-bacterial pathogens, mainly comprising MP and other pathogens (94%). There were significant differences in the total detection rate of IgM antibodies among all age groups (0-30 days: 50.0%; 1-6 months: 67.3%; 0.5-1 year: 33.1%; 1-3 years: 57.3%; 3-8.9 years: 61.7%). The positive rate of IgM antibodies against respiratory pathogens was highest in children with bronchial asthma, followed by children with asthmatic bronchitis, and it was lowest in children with bronchiolitis. IgM-positive children had significantly decreased blood eosinophils and significantly increased total serum IgE levels.
CONCLUSIONSThe main non-bacterial respiratory pathogens include MP, ADV and Flu B in children with asthmatic diseases, and co-infection of MP and other non-bacterial pathogens is common. Infants aged 1 to 6 months have a higher infection rate than other age groups. Monitoring the changes in total serum IgE levels and peripheral eosinophil count has great significance for the clinical diagnosis and treatment of asthmatic diseases in children.
Adenoviridae Infections ; diagnosis ; Age Factors ; Antibodies, Viral ; blood ; Asthma ; etiology ; microbiology ; virology ; Child ; Child, Preschool ; Eosinophils ; Female ; Fluorescent Antibody Technique, Indirect ; Humans ; Immunoglobulin E ; blood ; Immunoglobulin M ; blood ; Infant ; Infant, Newborn ; Male ; Pneumonia, Mycoplasma ; diagnosis