Leprosy is an infectious disease caused by Mycobacterium leprae. Five districts with the highest number of leprosy events, including the Camplong Subdistrict, have reported a continuous rise in the number of leprosy cases. This study aimed to analyze the relationship between the physical environment of the house, the presence of M. leprae DNA on the floor of the house and the presence of leprosy patients in Camplong Subdistrict, Sampang District. This study used a cross-sectional design. We collected data regarding 40 houses. The presence of M. leprae DNA in the floor samples was analyzed using the polymerase chain reaction (PCR) technique; 10% of soil samples showed the presence of M. leprae DNA. Variables associated with the presence of leprosy patients were temperature and the wall of the house. We concluded that that the presence of M. leprae does not depend on the presence of leprosy patients in the house although, theoretically, the soil may be a transmission medium for M. leprae. Therefore, everyone residing in an endemic area has the same risk of M. leprae exposure from the environment. We recommend that programs be conducted in endemic areas to raise the knowledge of the population about what constitutes a healthy house.
Mycobacterium leprae

The detection of structural ARN in ribosom-16sr RNA existing in living M.leprae has the value of valuating the efficacy of the treatment and make prognosis of the recurrence . NASBA is performed on 91 samples of biopsy of ear tissue of 2 groups of patients immediately after the treatment of the group I and 5 years after the treatment of the group II. In group I, the positive rate of NASBA is 1/29 and in group II is 3/62. All patients with positive NASBA are in the group of numerous bacilli (MB). In finishing the treatment, there is only 1 patient of MB group who has positive NASBA. Among 60 patients who finishes multichimiotherapy, after 5 years there are 3 patients with positive NASBA
Mycobacterium leprae ; Leprosy ; RNA

No abstract available.
Drug Resistance* ; Mycobacterium leprae* ; Mycobacterium*

Leprosy; Patients; Mycobacterium leprae; cells; Mycobacterium leprae; Antigens 18 leprosis patients treated in the Central Institute of Demato-venerlogy, 12 leprosis patients' children, 6 health workers in leprosis sanatoria enrolled medical students were enrolled in the study. ELISA technique was used to determine the process of production of specific antibody to ND-BSA in vitro. IgM antibody was detected in the culture of leprosis sample culture as well as of persons contacting with the patient and health persons. In each individual there can be IgM reaction, or only IgG and IgG together with IgM reaction to this antigene. All subjects who have IgG reaction to ND-BSA have transformed lympho response in vitro to ultracrushed M-leprae antigene and ND-BSA
Leprosy ; Patients ; Mycobacterium leprae ; cells ; Mycobacterium leprae ; Antigens

58 leprosis patients, aged 12-47 years old, treating in the South, were detected cellular immunity response to ultracrushed M. leprae and ND-BSA. Patients had positive response with ND-BSA. Cells having transformed responsed with ND-BSA are lymphocyte and in 5/12 patients there is a participation, mainly of subpopulation of T lymphocyte, there are Tgamadenta cells. When Tgamadenta was removed from cellular mixt, the absorption of H3-Thymidin uncreased dramatically in both ND-BSA nad BSA culture
Mycobacterium leprae ; Leprosy ; Diseases ; antigens

The zebrafish emerged as a new model of developmental research and infection. Recently zebrafish were used for various mycobacterial infection studies. In this study, we investigated a possibility of the zebrafish as an infection model of Mycobacterium leprae infection. We injected Mycobacterium leprae (10(5)/30microliter) to the peritoneum of zebrafish. After 4 weeks, we found two fish evident to the subsistence and multiplication of Mycobacterium leprae, among total 25 fish. This study thus demonstrates that zebrafish may become an animal model for M. leprae infection.
Leprosy* ; Models, Animal ; Mycobacterium leprae ; Peritoneum ; Zebrafish*

Using polymerase chain reaction (PCR) and sequencing, 492 nucleotides of pra gene was obtained from clinical swabs of the Vietnamese patients for comparative analysis with the corresponding sequences of the global strains of Mecobacterium leprae. As entry data for searching in Genbank, the Vietnamese strains of M.leprae showed very high homology level to the other global strains. In comparision to the standard TN strain, absolute homology (100%) for QH1 (designated as MLVN1) and 98-99% homology for QH2 strain (designated as MLVN2) of the Vietnamese M. leprae was observrd. There may be a number of variants among the M. leprae population in Vietnam. For final conclusion, thus, investigation must be done for many other isolates of patients and geographic origins. This is the first molecular-based identification for M. leprae in Vietnam. Our results afford establishing diagnostic and identification methodologies/techniques for M. leprae from clinical swabs from patients in Vietnam
Mycobacterium leprae ; Molecular Biology ; Polymerase Chain Reaction

Human ; Male ; Female ; Leprosy ; Mycobacterium Leprae

The nucleotide-oligomerization domain (NOD) is an important molecule involved in host defense against bacterial infection. To study the role of NODs in the host response to Mycobacterium leprae, we measured the mRNA levels of NODs and related genes in infected mouse tissues. The mRNA expression of NOD1, NOD2, caspase-1 and ASC was increased in mouse footpads. Whereas NOD2 expression in macrophages was increased at 2 and 24 h post-infection with M. leprae, there was no expression of NOD1 at these time points. An increase in caspase-1 expression was observed at 2 h and continued at 24 h. However, the expression of ASC was increased only at the early time point. The expression of caspase-1 is regulated by NOD2-dependent pathway in established HEK 293. Our results suggest NOD2, rather than NOD1, may be associated with the host response to M. leprae and that caspase-1 activation is essential for the host response.
Animals ; Bacterial Infections ; Macrophages ; Mice* ; Mycobacterium leprae* ; Mycobacterium* ; RNA, Messenger

Eight years before the Robert Koch's identification of Mycobacterium tuberculosis, Mycobacterium leprae, which is the firstly identified nontuberculous mycobacteria (NTM), was reported by G.H. Armauer Hansen in 1874. Thereafter a total of 71 species of Mycobacterium have been recognized or proposed. Despite the fact that NTM have been occasionally identified from clinical specimens, it is only recently that they draw a serious attention. In Korea, the frequency of isolation of NTM increased from 448 in 1992 to 1562 in 2002, while the prevalence of active tuberculosis over the same period decreased from 1.8% to 0.5%. The most commonly isolated NTM was M. avium-intracellulare complex (MAC) throughout the period of 1992~2002. M. abscessus was the second common, followed by M. fortuitum, M. gordonae and M. kansasii. In this article, we will overview the NTM-related lung diseases in terms of their diagnosis, clinical characteristics,and treatment.
Diagnosis* ; Gordonia Bacterium ; Korea ; Lung Diseases* ; Mycobacterium ; Mycobacterium leprae ; Mycobacterium tuberculosis ; Nontuberculous Mycobacteria ; Prevalence ; Tuberculosis