1.Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine.
Juyeon LEE ; Changjong MOON ; Jihoon KIM ; Chanwoo JUNG ; Keun Hwa LEE ; Hong Gu JOO ; Meejung AHN ; Taekyun SHIN
Journal of Veterinary Science 2009;10(3):177-180
The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.
Animals
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Cattle
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Cattle Diseases/*pathology
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Chronic Disease
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Galectin 3/*metabolism
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Immunohistochemistry
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Intestine, Small/microbiology/*pathology
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Mycobacterium avium subsp. paratuberculosis/growth & development/isolation & purification
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Paratuberculosis/*pathology
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RNA Polymerase II/genetics