Electromagnetic Fields ; adverse effects ; Humans ; Mutagenicity Tests

The overseas and domestic application status and research progress of genotoxicity evaluation of medical devices is briefly introduced. The trend of future development is analyzed.
Equipment and Supplies ; adverse effects ; Mutagenicity Tests

This study deals with the serious pollution of Seoul's ambient air and extends a warning regarding its adverse effects on human health. The collected dust samples exceeded the legal standard (15O microgram/m3 of total suspended particulates) of ambient air quality. Fine particles, with an aerodynamic diameter of less than 2.5 micrometer comprised on the average 75.4 percent of the total suspended particulates (TSP). The amount of ether extractable organic matter (EEOM) of the fine particles was found to range from 3.6 to 7.1 percent. Neutral, acidic and basic organics were fractionated. In the neutral fraction, aliphatic compounds (ALP), polyaromatic hydrocarbons0 (PAH), and polar neutral organic compounds (POCN) comprised 23.1, 37.8 and 39.1 percent, respectively. Mutagenic activities of the organic fractions were determined by Ames bioassay. PAH showed the most mutagenicity using Salmonella typhimurium TA98 strain and TA98NR(nitro-reductase activity deficient stain) in the presence of S-9 fractions.
Dust/analysis* ; Human ; Korea ; Mutagenicity Tests* ; Polycyclic Hydrocarbons/analysis*

Cell Phone ; DNA Damage ; Electromagnetic Fields ; adverse effects ; Mutagenicity Tests

DNA Damage ; drug effects ; Formaldehyde ; toxicity ; Mutagenicity Tests

Mutagenicity Tests ; methods ; Tobacco Smoke Pollution ; adverse effects

Animals ; Carcinogenicity Tests ; Cytotoxicity Tests, Immunologic ; Humans ; Mutagenicity Tests ; Soot ; toxicity

Genotoxicity research takes an important place in traditional Chinese medicine safety evaluation. Genotoxicity test on traditional Chinese medicine has been paid great attention since 1970s. Currently, the most developed genotoxicity test methods included: bacterial reverse mutation test and mouse lymphoma assay which are used to detect relevant genetic changes, micronucleus test and chromosomal analysis which are used to measure chromosomal aberration, and single cell electrophoresis assay which is used to test DNA damage. This article reviews research progress on genotoxicity of traditional Chinese medicine, evaluation methods of genotoxicity, the problems and solutions on genotoxicity evaluation of traditional Chinese medicine, and new technique used in genotoxicity test.
Animals ; Biomedical Research ; Humans ; Medicine, Chinese Traditional ; adverse effects ; Mutagenicity Tests ; methods

This study evaluates the cytotoxic and mutagenic effect of synthetic hydroxyapatite granules (source: School of Material and Mineral Resources Engineering, Universiti Sains Malaysia) in the bone marrow cells of mice. Mice are exposed to synthetic hydroxyapatite granules, the bone marrow cells are collected and observed for chromosome aberrations. No chromosome aberrations were noticed in the animals exposed to distilled water (negative control) and to the test substance, synthetic hydroxyapatite granules (treatment) groups. Chromosome aberrations were observed in the animals exposed to Mitomycin C (positive control group). There was no indication of cytotoxicity due to synthetic hydroxyapatite granules in the animals as revealed by the mitotic index. Hence, synthetic hydroxyapatite granules are considered non-mutagenic under the prevailing test conditions.
Bone Marrow Cells/*drug effects ; Bone Substitutes/*toxicity ; *Chromosome Aberrations ; Durapatite/*toxicity ; *Mutagenicity Tests

Carcinogenesis is a complex process involved in genotoxic and non-genotoxic pathways. The carcinogenic potential of silver nanoparticles (AgNPs) has been predicted by examining their genotoxic effects using several in vitro and in vivo models. However, there is no little information regarding the non-genotoxic effects of AgNPs related to carcinogenesis. The in vitro cell transformation assay (CTA) provides specific and sensitive evidence for predicting the tumorigenic potential of a chemical, which cannot be obtained by genotoxicity testing. Therefore, we carried out CTA in Balb/c 3T3 A31-1-1 cells to evaluate the carcinogenic potential of AgNPs. Colony-forming efficiency and crystal violet assays were carried out to determine the cytotoxicity of AgNPs. A cytokinesis-block micronucleus (CBMN) assay and CTA were performed using Balb/c 3T3 A31-1-1 cells to predict the in vitro carcinogenic potential of AgNPs. In the CBMN assay, AgNPs (10.6 μg/mL) induced a significant increase in micronucleus formation indicating a genotoxic effect. Thus, AgNPs could be an initiator of carcinogenesis. In the CTA, used to assess the carcinogenic potential of AgNPs, cells exposed to AgNPs for 72 hours showed significantly induced morphological neoplastic transformation at all tested doses (0.17, 0.66, 2.65, 5.30, and 10.60 μg/mL), and the transformation frequency was significantly increased in a dose-dependent manner. These results indicate that short-term exposure (72 hours) to AgNPs had in vitro carcinogenetic potency in Balb/c 3T3 A31-1-1 cells.
Animals ; Carcinogenesis ; Gentian Violet ; In Vitro Techniques ; Mice ; Mutagenicity Tests ; Nanoparticles ; Silver