Abstract: To comparatively study the effect of three roots talh(pulvis compositus) medicine and three roots granule medicine in the immune system. Materials and Methods: A total number of 32 healthy Wistar male rats weighing 180-220 grams were taken and equally divided into four groups: healthy group; small dose (0.85g/kg) of three roots talh medicine group(TRSD); high dose (1.7g/kg) of three roots talh medicine group(TRHD) and three roots granule medicine group at dose 1.3g / kg(TRGM). Medicine was given once every day for 42 days. At the end of the study, spleen index and thymus index were calculated and IL-2, IL-6, TNF-α, iNOS, NO levels in blood plasma and spleen using ELISA(Magellan 7.2) was determined and compared with healthy group. Result: There was no statistically significant increase in spleen index and thymus index between the healthy group and medicine groups(p>0.05). In blood plasma, there was no statistically significant difference in IL-2, IL-6, TNF-α, iNOS and NO between healthy and medicine groups (P> 0.05).
In spleen, there was significant increased in IL-2 in the high dose three roots talh medicine group in compare with healthy group (p<0.05). Similarly, IL-2, IL-6 and TNF-α was significantly increased in three roots granule medicine group in comapre with healthy group (P<0.05; P<0.05; P>0.001 respectively). There was no significant difference in iNOS and NO between healthy and medicine groups (p>0.05). Conclusions Three roots talh medicine and three roots granule medicine has active of immune system and three roots granule medicine has more activates the immune system compared to the three roots talh medicine.