An activation domain in p67(phox) (residues 199-210) is critical for regulating NADPH oxidase activity in cell-free system [10] To determine the steady state reduction of FAD, thioacetamide-FAD was reconstituted in gp91(phox), and the fluorescence of its oxidised form was monitored. Omission of p67(phox) decreased the steady state reduction of the FAD from 28% to 4%, but omission of p47(phox) had little effect. A series of the truncated forms of p67(phox) were expressed in E.coli to determine the domain in p67(phox) which is essential for regulating the steady state of FAD reduction. The minimal length of p67(phox) for for regulating the steady state of FAD reduction is shown to be 1-210 using a series of truncation mutants which indicates that the region 199-210 is also important for regulating electron flow within flavocytochrome b(558). The deletion of this domain not only decreased the superoxide generation but also decreased the steady state of FAD reduction. Therefore, the activation domain on p67(phox) regulates the reductive half-reaction for FAD, consistent with a dominant effect on hydride/electron transfer from NADPH to FAD.
Amino Acid Sequence ; Base Sequence ; Cell Membrane/metabolism ; Cell-Free System ; DNA Primers ; Flavin-Adenine Dinucleotide/*metabolism ; Humans ; Kinetics ; Membrane Glycoproteins/*metabolism ; Molecular Sequence Data ; NADH Dehydrogenase/metabolism ; *NADPH Oxidase ; Neutrophils/enzymology/metabolism ; Oxidation-Reduction ; Peptide Fragments/chemistry ; Phosphoproteins/*chemistry/*metabolism ; Polymerase Chain Reaction ; Sequence Deletion

Many procedures of quadricepsplasty were reported by Thompson (1944), Judet (1959), van Nes (1962), Nicoll (1963) and Hesketh (1963) in confining the indication of them for the strictly extra-articular origin of their causes. During the period of January 1979 to August 1979, 5 cases of stiff knee joints were surgically treated by means of arthrolysis and quadriceps release. The result were gratifying. The average gain of flexion was 119 degrees, and extension lag was absent except a case which had compression fracture of femoral condyle.
Fractures, Compression ; Knee Joint ; Knee

Larsen's syndrome is a very rare deformity, which is characterized by multiple congenital dislocations with a characteristic facial abnormality. The authors experienced a case of typical Larsen's syndrome that had saddle nose, hypertelorism, high-arched palate, dislocation of the hip joint, subluxation of the knee joint, dislocation of both radial heads, equinovarus deformities of both feet and winged scapulae of both shoulders. The dislocated left hip joint was reduced surgically and the result was excellent.
Clubfoot ; Congenital Abnormalities ; Dislocations ; Foot ; Head ; Hip Joint ; Hypertelorism ; Knee Joint ; Nose ; Palate ; Scapula ; Shoulder

The clinical value of CT in the diagnosis of pelvic bone fractures is being explored. Conventional radiographic projections, including internal and external oblique views, although very helpful, often cannot provide sufficent jnformation to give a true sense of the fractures. CT conversely displays the anatomy in an axial plane, thereby providing the missing pieces of information and fascilitating classification of these fractures. From January 1983 to June 1986, 34 patients with pelvic bone fractures, admitted to Department of Orthopedic Surgery, Severance Hospital, were given both CT and conventional radiographs. Comparing the conventional radiographic findings with those of CT, the following conclusions were obtained. 1. For patients with multiple trauma who necessitate position changes during conventional radiography, no further change in position was required during CT. 2. CT clearly showed intraarticular loose bodies, anterior wall fractures of the acetabulum, and separations of the saicroiliac joint which were hardly detected on conventional radiographs. 3. CT permitted a better evaluation of the shape, extent, and degree of separation of fracture fragment, so it was very helpful in formulating the treatment plan. 4. Follow-up CT was helpful in evaluating the effectiveness of treatment.
Acetabulum ; Classification ; Diagnosis ; Follow-Up Studies ; Humans ; Joints ; Multiple Trauma ; Orthopedics ; Pelvic Bones ; Radiography

No abstract available.
Immunoglobulins* ; Tuberculosis, Pulmonary*

Buerger's disease was first identified in 1908 by Leo Buerger as a distinct clinicopathological entity, which causes severe pain and necrosis on distal parts of the extremities and occurs especially in young male adults. In spite of many treatment modalities, it is not completely cured but results in amputation in the long run. So, we studied a total of 120 cases of Buerger's disease, who were admitted at Yonsei University, Severance Hospital from Jan., 1975 to June, 1985 and we obtained the following results. 1. All the patients were male and majority of them were under 40 years of age (69.2%). 2. 118 cases were smokers and 2 cases were non-smokers. 3. The initial symptoms were resting pain, necrosis, and color change in descending order of frequency. 4. Diagnoses were made mainly by angiogram(75.8%) and most common site of obstruction was popliteal artery (49.4%). 5. After sympathectomy and angioplasty, there was temporary relief of symptoms, but none showed good results after long term follow up. As a result, amputations were performed after sympathectomy in 47 cases(39.2%), of which 36 cases(76.6%) received that surgery within 2 yeas after sympathectomy. 6. The pressure index not only helps diagnose the disease but also is a very useful method to objectively assess the severity of the initial symptoms and postoperative results.
Adult ; Amputation ; Angioplasty ; Clinical Study ; Diagnosis ; Extremities ; Follow-Up Studies ; Humans ; Male ; Methods ; Necrosis ; Popliteal Artery ; Sympathectomy ; Thromboangiitis Obliterans

This retrospective study was performed to assess CT scan findings of pancratic cancer and its ability todetermine resectability. CT scans of 41 patients, who were diagnosed to have pancreatic cancer on histological orclinical base, were reviewed. Most common findings were; focal pancreatic enlargement, diffuse pancreaticenlargement, loss of distinct contour, peripancretic fat obliteration, bile duct or pancreatic duct dilatation,vascular invasion, lymph node involvement, direct invasion of adjacent organs and distant metastasis.Resectability was assessed according to generally accepted CT findings such as small pancreatic mass lesion havingnormal lobulated contour of pancreas, no evidence of vascular invasion, no evidence lymph node involvement andfree of distant metastasis. With the criteria, 5 cases among 41 cases deemed to be resectable. However onoperation all cases were found unresectable indicating prediction of tumor resectability is difficult even withhelp of CT. Nonetheless CT would be very helpful in management of patients having pancreatic cancer since CT wouldshorten the diagnostic procedures of pancreatic cancer.
Bile Ducts ; Humans ; Lymph Nodes ; Neoplasm Metastasis ; Pancreas ; Pancreatic Ducts ; Pancreatic Neoplasms ; Retrospective Studies ; Tomography, X-Ray Computed

Primary hyperparathyroidism is a rare endocrine disease in children. It involves bone and joint, urinary tract, gastrointestinal tract and cardiovascular system. The main cause of these involvement is high level of PTH in serum, resulting in hypercalcemia.An 11 years old male patient who had complained of limping gait since last 18 months, showed typical laboratory and radiological findings of primary hyperparathyroidism. At the ultrasonography, computed tomography and radionuclide scanning, a well defined mass(10 X 15mm) was found on the posterior aspect of the right thyroid lobe. The mass was confirmed histologically as adenoma of parathyroid gland. The patient was successfully treated with subtotal parathyroidectomy and temporal administration of calcium and vitamin D.We report this case of primary hyperparathyroidism with brief review of the literatures.
Adenoma ; Calcium ; Cardiovascular System ; Child ; Endocrine System Diseases ; Gait ; Gastrointestinal Tract ; Humans ; Hyperparathyroidism, Primary ; Joints ; Male ; Parathyroid Glands ; Parathyroidectomy ; Thyroid Gland ; Ultrasonography ; Urinary Tract ; Vitamins

Twenty four cases of acromioclavicular seperation were admitted and treated at Severance Hospital, Yonsei University from October 1964 to September 1975. Of these, six cases were subluxations and eighteen cases were dislocations. Acromioclavicular joint injury is relatively rare and there are many methods of treatment. In our cases, all the six subluxations and seven of the eighteen dislocations were treated by conservative method, while eleven of the eighteen dislocations were treated by operative method. The results of all the subluxated cases were satisfactory. In cases of dislocation, the results were considerably better in operated cases with Weavers method.
Acromioclavicular Joint ; Dislocations ; Methods

No abstract available.
Dopaminergic Neurons* ; Retina*