Microbial transformation of (±)-6-(1,1-dimethylallyl)naringenin (6-DMAN, 1) and (±)-5-(O-prenyl) naringenin-4′,7-diacetate (5-O-PN, 2) was performed by using fungi. Scale-up fermentation studies with Mucor hiemalis, Cunninghamella elegans var. elegans, and Penicillium chrysogenum led to the isolation of five microbial metabolites. Chemical structures of the metabolites were determined by spectral analyses as (±)-8-prenylnaringenin (3), (2S)-5,4′-dihydroxy-7,8-[(R)-2-(1-hydroxy-1-methylethyl)-2,3-dihydrofurano]flavanone (4), (±)-5-(O-prenyl)naringenin-4′-acetate (5), (±)-naringenin-4′-acetate (6), and (±)-naringenin (7), of which 5 was identified as a new compound.
Cunninghamella ; Fermentation ; Fungi ; Mucor ; Penicillium chrysogenum

Aspergillosis ; Humans ; Mucor ; Mucormycosis/drug therapy*

During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.
DNA, Ribosomal ; Fresh Water ; Korea* ; Mucor* ; Mucorales ; Phylogeny ; Soil

Agrocybe aegerita is an important mushroom cultivated in Korea, with good feel and a peculiar fragrance. A. aegerita can be cultivated throughout the year using culture bottles but is more susceptible to contamination than other mushrooms. Twenty-two pathogens were isolated from the fruiting bodies and compost of A. aegerita, and seven isolates were isolated from Pleurotus ostreatus to compare with the A. aegerita isolates, collected from Gimje, Iksan, Gunsan of Chonbuk, and Chilgok of Gyeongbuk Province in 2009. These isolates were identified based on morphological and molecular characteristics. Of the 29 isolates, 26 were identified as Trichoderma spp. and the remaining three were Aspergillus spp., Mucor spp., and Penicillium spp. A phylogenetic analysis revealed that the 26 isolates of Trichoderma were divided into four taxa, namely T. harzianum, T. pleuroticola, T. longibrachiatum, and T. atroviride. Among the Trichoderma spp., 16 isolates (55.2%) were identified as T. harzianum, six as T. pleuroticola (20.7%), two as T. longibrachiatum, and the remaining two were T. atroviride.
Agaricales ; Agrocybe ; Aspergillus ; Fruit ; Korea ; Mucor ; Penicillium ; Pleurotus ; Soil ; Trichoderma

Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.
Agaricales* ; Cladosporium ; DNA ; Fungi ; Mucor ; Pleurotus* ; Polymerase Chain Reaction ; Trichoderma

Rhino-orbital mucormycosis is the most frequently fatal fungal infection commonly occurring in patients with poorly controlled diabetes mellitus and and those who are immunocompromised, and requires prompt treatment. We describe a case of rhino-or-bital mucormycosis with orbital cellulitis and paranasal sinusitis, as seen on initial MR images, which on follow-up images had evol ved to orbitofacial infarction. MR imaging was useful for the demonstration of orbitofacial infarction, seen as areas of lack of enhancement and thus suggesting vascular invo l vement by mucor hyphae.
Diabetes Mellitus ; Follow-Up Studies ; Humans ; Hyphae ; Infarction* ; Magnetic Resonance Imaging* ; Mucor ; Mucormycosis* ; Orbital Cellulitis ; Sinusitis

BACKGROUND: Because of recent concern about occurrence of otomycosis in otolaryngologic practice, we studied them. OBJECTIVE: The purpose of this study was to investigate clinical and mycological features of otomycosis. METHOD: We evaluated the clinical and mycological aspects of otomycosis in 38 patients from March 1996 to February 1998 in Dongguk University Hospital. RESULTS AND CONCLUSION: Otomycosis showed high incidence in the twenties (23.7%). The ratio of male to female patient was 1:1.5. The seasonal prevalence was highest in autumn. Most common symptoms were otorrhea (39.4%) followed by itching, obstruction sensation, otalgia, and hearing disturbance. The common causative organisms of otomycosis were Aspergillus(A.) niger (36.8%) followed by A. terreus (26.3%), A. flavus (21.1%), A. fumigatus (7.9%), Candida albicans (5.3%), and Mucor sp. (2.6%).
Candida albicans ; Earache ; Female ; Hearing ; Humans ; Incidence ; Male ; Mucor ; Niger ; Otomycosis* ; Prevalence ; Pruritus ; Seasons ; Sensation

The mycotic flora of the conjunctival sac was investigated in the following fhie groups: Group I: Control group: 60 cases. Group II: Children group (3 to 4 years of age): 80 eyes. Group III: Young adult group (40 to 70 years of age): 60 eyes. Group IV: Old aged group(40 to 70 years of age): 60 eyes. Group V: Young adult group treated with 0.5% cortisone acetate solution and 0.5% chloramphenicol solution, q.i.d., for three weeks: 104 eyes. Conjunctival stroke was made at the lower conjunctival fornix of each eye and inoculated on Sabouraud's glucose media. In group I. the cotton stick was not contacted with the conjunctiva, but the similar procedure was performed, as in the other groups, remaining in the air for the same duration to rule out air contamination. The fungi cultured for at least four weeks were identified by macroscopic appearance the colonies and microscopic findings of the mycelia and spores. The following results were obtained: 1) No positive culture was noted in Group I. This will rule out contamination during the procedure. 2) Positive cultures were observed in 16.2% of normal adults, and in 6.2% of normal children. 3) In steroid and antibiotic treated group, the incidence of positive cultures increased to 31.7%. 4) The fungi cultured were identified as the following eight species: Penicllium sp., Aspergillus sp., Mycelia sterila., Hormodendrum sp., Mucor sp., Cephalosporium sp., and Oospora sp.
Acremonium ; Adult ; Aspergillus ; Child ; Chloramphenicol ; Conjunctiva* ; Cortisone ; Fungi ; Glucose ; Humans ; Incidence ; Mucor ; Spores ; Stroke ; Young Adult

While surveying undiscovered fungal taxa in Korea, three rare zygomycetous fungal strains, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were isolated from soil, leaf, and freshwater samples, respectively. The strains were analyzed morphologically as well as phylogenetically based on the internal transcribed spacer region and 28S rDNA sequences. Sequence analysis of the two loci revealed that the isolates, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were identified as Backusella circina, Circinella muscae, and Mucor ramosissimus, respectively. These species have not yet been previously described in Korea.
Classification ; DNA, Ribosomal ; Fresh Water ; Fungi* ; Korea* ; Mucor* ; Sequence Analysis ; Soil

A soft rot of fruits caused by Mucor racemosus occurred on cherry tomato collected in Agricultural Products Wholesale Market in Jinju, Korea. The disease infection usually occurred wounded areas after cracking of fruits. At first, the lesions started with water soaked and rapidly softened and diseased lesion gradually expanded. Colonies were white to brownish to gray in color. Sporangia were 32~54 microm in size and globose in shape. Sporangiophores were 8~14 microm in width. Sporangiospores were 5~12 x 4~8 microm in size, ellipsoidal to subglobose in shape. Columella was 27~42 microm in size, obovoid, ellipsoidal, cylindrical-ellipsoidal, slightly pyriform in shape. Chlamydospores were numerous in sporangiophores and barrelshaped when young, subglobose in old cultures. Optimum growth temperature was about 25degrees C. The fungus was identified as M. racemosus Fres. This is the first report of soft rot on cherry tomato caused by M. racemosus in Korea.
Fruit ; Fungi ; Gyeongsangnam-do ; Korea* ; Lycopersicon esculentum* ; Mucor* ; Prunus ; Sporangia ; Water ; Wounds and Injuries