3.Two Cases of Epithelioid Sarcoma with Immunohistochemical Study.
Duk Kyu CHUN ; Kwang Hyun CHO ; Seong Hoe PARK
Annals of Dermatology 1992;4(1):26-31
Two typical cases of epithelioid sarcoma were examined by immunohistochemical stain using antibodies to epithelial membrane antigen, carcinoembryonic antigen, vimentin and cytokeratin. Both cases showed positive reactivity for the four kinds of antibodies. These results point to the fact that epithelioid sarcoma simultaneously expresses epithelial markers and characteristic mesenchymal phenotypes. Epithelioid sarcoma appears to be a tumor derived from a multipotential mesenchymal cell with multidirectional differentiation.
Antibodies
;
Carcinoembryonic Antigen
;
Keratins
;
Mucin-1
;
Phenotype
;
Sarcoma*
;
Vimentin
4.Immunohistochemical Study on Acral Lentiginous Melanoma.
You Chan KIM ; Min Geol LEE ; Sang Ho CHO ; Sung Whan CHOE ; Hyang Joon PARK ; Yong Woo CINN
Korean Journal of Dermatology 2002;40(6):620-625
BACKGROUND: Although clinicopathologic characteristics of acral lentiginous melanoma (ALM) is well established, immunohistochemical study on ALM has rarely been reported. OBJECTIVE: Our purpose is to evaluate the usefulness of several immune markers in the diagnosis of ALM. METHODS: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, vimentin, epithelial membrane antigen (EMA), and CAM 5.2. RESULTS: 1. Nineteen (95%) and 16 (80%) out of 20 ALM showed reactivity with S-100 protein and HMB-45, respectively. 2. Melanin bleaching was useful for diagnosing heavily pigmented ALM using both S-100 protein and HMB-45. 3. The immunoreactivity of S-100 protein and HMB-45 did not correlate with tumor thickness or level of invasion of ALM. The intensity of HMB-45 correlated well with the melanin content. 4. One and 2 out of 20 cases stained focally with EMA and CAM5.2 respectively, but these cases stained also with HMB-45 and/or S-100 protein. CONCLUSION: S-100 protein and HMB-45 were relatively sensitive markers for diagnosing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, S-100 protein and HMB-45 are very useful markers for diagnosing ALM.
Biomarkers
;
Diagnosis
;
Melanins
;
Melanoma*
;
Mucin-1
;
Paraffin
;
S100 Proteins
;
Vimentin
5.Microcystic Meningioma - Unusual Variant of Meningiomas.
Jun Hyung CHO ; Kook Hee YANG ; Ho Yeal ZHANG ; Jeong Hae KIE
Journal of Korean Neurosurgical Society 2003;34(4):382-385
Microcystic meningioma is a rare variant of meningiomas. This unusual variant was originally described by Masson, who labeled it "humid". The computed tomographic scan or magnetic resonance images of these tumors resemble those of a glial or metastatic tumor with cystic or necrotic changes. There is no definitive method for differentiating cystic meningiomas from these more common tumors. But immunohistochemically, they share a similar pattern of positive staining for epithelial membrane antigen and vimentin with other meningiomas. Our case was a 34-year-old woman with a tumor mass on the right frontal area. She was admitted to hospital because of generalized tonic seizure. Grossly all of the tumor could be removed, and histopathologically this tumor was revealed to be a microcystic meningioma.
Adult
;
Female
;
Humans
;
Meningioma*
;
Mucin-1
;
Seizures
;
Vimentin
6.Expression of MUC1 and MUC4 and Its Prognostic Significance in Non-Small Cell Lung Carcinoma.
Ji Min JEON ; Hye Won LEE ; Ji Young PARK ; Hye Ra JUNG ; Ilseon HWANG ; Sun Young KWON ; Mi Sun CHOE ; Yu Na KANG ; Sang Pyo KIM ; Sang Sook LEE ; Won Il CHOI ; Kun Young KWON
Korean Journal of Pathology 2010;44(4):397-403
BACKGROUND: Mucin (MUC)1 and MUC4 (MUC1, 4) are high molecular weight glycoproteins expressed in normal and malignant epithelial cells, and these expressions are related to the prognosis of some carcinomas. In non-small cell lung carcinoma (NSCLC), the relationship between MUC1, 4 expressions and their prognostic significance is not well known. We evaluated these relationships in a series of NSCLC: 1) between MUC1, 4 expression levels and histologic subtypes, and 2) between high expression of MUC1, 4 and their prognostic significance. METHODS: We performed immunohistochemical staining for MUC1, 4 in paraffin-embedded tissues from 165 NSCLC cases arranged in a tissue microarray. RESULTS: We found a significant correlation between MUC1, 4 expressions and NSCLC histologic subtypes (p < 0.05). High MUC1 expression was characteristic of adenocarcinoma. Low MUC1, 4 expressions were characteristic of squamous cell carcinoma. In adenocarcinoma, we found significant association between diffuse MUC1 expression and short patient survival (p = 0.005). In squamous cell carcinoma, diffuse MUC4 expression showed long patient survival trend (p = 0.128). CONCLUSIONS: MUC1, 4 expression levels were significantly correlated with NSCLC histologic subtypes. Diffuse MUC1 expression was significantly associated with shortened survival in NSCLC patients, especially in adenocarcinoma.
Adenocarcinoma
;
Carcinoma, Squamous Cell
;
Epithelial Cells
;
Glycoproteins
;
Humans
;
Lung
;
Molecular Weight
;
Mucin-1
;
Mucin-4
;
Mucins
;
Prognosis
7.The Expression and Distribution of MUC1 in Human Corneal Epithelium.
So Young KIM ; Seung Eun JUNG ; Kyoung Yul SEO ; Ja Hyun BAIK ; Jong Eun LEE ; Eung Kweon KIM
Journal of the Korean Ophthalmological Society 2001;42(1):145-151
Corneal and conjunctival squamous epithelial cells have been known to express the mucin MUC1. We attempted to reveal the expression and localizational characteristics of the membrane-spanning mucin MUC1 as a component of the mucous layer in the human corneal epithelium. An antibody to the MUC1 was used to detect the MUC1 on the corneal epithelium by immunohistochemistry and immunofluorescent staining. In situ hybridization was performed to determine the distribution of MUC1 mRNA in the ocular surface. Immunohistochemically, the MUC1 mucin was observed along the apical membranes of the corneal epithelium. According to immunofluorescent staining, cells varied in the amount of mucin MUC1. Expression of MUC1 mRNA was observed in all layers of the corneal epithelium. The MUC1 mucin synthesized by the corneal epithelia exists on the apical membrane of the superficial cells. The amount of MUC1 may vary with the vertical migration and the activity of the cells.
Epithelial Cells
;
Epithelium, Corneal*
;
Humans*
;
Immunohistochemistry
;
In Situ Hybridization
;
Membranes
;
Mucin-1
;
Mucins
;
RNA, Messenger
8.Immunohistochemical Analysis for the Origin of Mammary Paget's DiseaseSang.
Yoon LEE ; Woo Chan PARK ; Young Jin SEO ; Seung Hye CHOI ; Sang Seol JUNG
Journal of the Korean Surgical Society 1998;55(1):28-34
James Paget issued the first report that nipple symptoms were related to breast carcinoma; however, the histogenesis of mammary Paget cells is still controversial. Toker presented evidence for epidermotropic migration of Paget cells. An other main theory hypothesizes that the Paget cell originates in the intraepidermis and is to be considered an independent in situ carcinoma, possibly as a multicentric breast cancer. The authors examined fifteen cases of Paget's disease in the breast for the immunohistochemical epithelial membrane antigen, glandular low-molecular-weight cytokeratin, epidermal high- molecular-weight cytokeratin, and carcinoembryonic antigen in order to determine the origin of the histogenesis of the Paget cell. All specimens revealed Paget cells strongly reactive with the epithelial membrane antigen. Thirteen out of the fifteen cases showed a strong reaction with the glandular cytokeratin. Four out of the fifteen cases showed a moderate reaction with the carcinoembryonic antigen. All specimens showed a negative reaction with the epidermal cytokeratin. With the above results, the authors conclud that mammary Paget cells may originate from the underlying breast tissue and suggest a radical mastectomy for Paget's disease with the underlying invasive cancer and conservative surgery for the disease with intraductal cancer in the breast.
Breast
;
Breast Neoplasms
;
Carcinoembryonic Antigen
;
Keratins
;
Mastectomy, Radical
;
Mucin-1
;
Nipples
9.A Case of Extraocular Sebaceous Carcinoma Arising from Nevus Sebaceus.
Jae Hui NAM ; Young Jun CHOI ; Tae Hwan KIM ; Ga Young LEE ; Won Serk KIM ; Kea Jeung KIM
Korean Journal of Dermatology 2009;47(3):326-329
Sebaceous carcinoma (SC) is a slow-growing, locally aggressive tumor with the high potency of metastatic spread. This neoplasm frequently arises from the sebaceous glands of the eyelid and exceptionally from extraocular sites. Approximately one-quarter of all reported cases of SC are extraocular. We observed a 42-year-old woman with an crusted erythematous to brownish plaque on her scalp. The biopsy specimen showed that multiple lobules were composed of foamy-cytoplasmic cells and sebocytes which show pleomorphism and atypia. For the immunohistochemistry, abundant tumor cells (with the expressions of epithelial membrane antigens and cytokeratin) were found within the masses. We present here a case of SC of the scalp arising from a previous lesion clinically compatible with a nevus sebaceous.
Adult
;
Biopsy
;
Eyelids
;
Female
;
Humans
;
Immunohistochemistry
;
Mucin-1
;
Nevus
;
Scalp
;
Sebaceous Glands
10.A Case of Malignant Eccrine Poronta.
Joung Soo KIM ; Young Suck RO ; Chan Kum PARK
Korean Journal of Dermatology 1998;36(4):717-721
Malignant eccrine poroma is a rare malignant tumor arising from the intraepidermal ductal portion of the eccrine sweat gland, which develops either spontaneously or from a long-standing benign eccrine poroma. It usually affects older people and is located most commonly on the lower extremities. We describe a typical case of malignant eccrine poroma developing from benign eccrine poroma, which had been present for 10 years or more, with subsequent regional lymph node metastasis. A biopsy specimen revealed many well-defined intraepidermal nests of poroma cells with dermal invasion and lymphatic involvement of tumor cells. Immunohistochemical studies showed staining with cocktail cytokeratin, carcino-embryonic antigen, epithelial membrane antigen in intracellular vacuoles of tumor cells and early embryonic stages of eccrine duct formation. Local excision with postoperative adjuvant radiation therapy showed only a temporary response.
Biopsy
;
Eccrine Porocarcinoma
;
Keratins
;
Lower Extremity
;
Lymph Nodes
;
Mucin-1
;
Neoplasm Metastasis
;
Poroma
;
Sweat Glands
;
Vacuoles