1.Application of computer-assisted telephone intervewing in the follow-up study of Keshan disease
Chinese Journal of Endemiology 2012;31(4):448-451
Objective To explore the application of computer-assisted telephone interviewing in the follow-up study of endemic diseases,and to provide a reference and basis for wide range application of this method.Methods In this investigation,146 patients with Keshan disease from 2 provinces were recruited in.The study mainly included two questionnaire surveys:disease follow-up questionnaire survey and cardiac insufficiency quality of life scale survey.Survey methods included computer-assisted telephone survey and face-to-face questionnaire survey.In the 18-month of follow-up study,one computer-assisted telephone survey was done in the 3ed,6th,12th,15th month,and one face-to-face questionnaire survey in 9th and 18th month.Time-consuming and quality of the two survey methods were eompaired.The computer-assisted telephone survey database was designed using Epi Info 3.32software.Results The computer-assisted telephone survey took an average of (13.10 t 1.80)min,and face-to-facequestionnaire survey took an average of (22.04 ± 2.28)min,the difference was statistically significant (t =- 36.214,P < 0.05).The error rate of computer-assisted telephone survey was 6.80% (37/544),and that of face-to-face questionnaire survey was 11.07%(28/253),the difference was statistically significant(x2 =4.195,P < 0.05).The quality of computer-assisted telephone survey was better than that of face-to-face questionnaire survey.Conclusions Through the computer-assisted telephone interviewing,the work efficiency of investigators is improved during the follow-up study of Keshan disease.The validation function improves the accuracy rate during the process of data entry.Because of the application of telephone contact,a lot of research funding is saved.
2.Study and evaluation of preparation of silybin PLGA microspheres by stainless steel membrane emulsification technique.
Kun RONG ; Bin-Li LIU ; Mu-Zi LI ; Liang-Liang XU ; Xin YI ; Cheng-Ke CAI
China Journal of Chinese Materia Medica 2014;39(7):1229-1233
OBJECTIVEThe aim of the present study was to prepare uniform-sized silybin loaded poly (lactic-co-glycolic acid) (PLGA) microspheres in study of silybin with stainless steel membrane.
METHODSilybin PLGA microspheres were prepared by stainless steel membrane emulsification. The preparation conditions were optimized by single-factor test and orthogonal experiment, and evaluating the mean diameters, the particle size distribution, drug loading, entrapment efficiency and morphology of microsphere.
RESULTPrepared microspheres were round and surface was smooth. The mean diameter was (4.961 +/- 0.56) microm. The span was (1.75 +/- 0.18). The entrapment efficiency was (54.997 +/- 4.05)% and the average drug loading was (23.6 +/- 1.70)%.
CONCLUSIONThe stainless steel membrane emulsification can be used to prepare the silybin PLGA microspheres. The mean diameters of the silybin PLGA microspheres can be controlled in certain level. Stainless steel membrane emulsification has great potentiality exploitation and utilization.
Drug Compounding ; methods ; Emulsions ; chemistry ; Lactic Acid ; chemistry ; Microspheres ; Particle Size ; Polyglycolic Acid ; chemistry ; Silymarin ; chemistry ; Stainless Steel ; chemistry
3.Co-delivery of Tim-3 mAb and sorafenib enhanced chemoimmunotherapy for hepatocellular carcinoma using "responsive shell-peeling" mesoporous silica nanoparticles
Sheng-jun MU ; Xiao SANG ; Wei-wei MU ; Tian-qi WANG ; Rui YANG ; Zi-peng ZHANG ; Yong-jun LIU ; Na ZHANG
Acta Pharmaceutica Sinica 2022;57(1):200-210
Chemoimmunotherapy has attracted much attention as an emerging therapy pattern for the treatment of cancers. Exploring effective drug combination schemes and reasonable delivery methods remained the key issue in current research. Herein, we designed sorafenib (SF) and anti-Tim-3 monoclonal antibody (Tim-3 mAb) co-loaded MMP2-responsive mesoporous silica nanoparticles (ST-MSNs) for combined chemoimmunotherapy of hepatocellular carcinoma (HCC). The shell of ST-MSNs was fabricated by Tim-3 mAb through matrix metalloproteinase 2 (MMP2) sensitive peptides as "gatekeepers" to prevent drug release during the blood circulation. In tumor microenvironment, the high levels of MMP2 caused the responsive shedding of Tim-3 mAb, leading to the triggerred release of SF and Tim-3 mAb. Then, SF could be delivered to tumor cells and Tim-3 mAb could be delivered to T cells, respectively.
4.Thoughts on Application of Evaluation Index System on Human Research Ethics Management in Beijing Medical Institutions
Zhengjuan HU ; Yiting LI ; Fang LIU ; Xiaoxia PENG ; Yingwei SUN ; Xiaolong MA ; Mingjie ZI ; Zhuoran ZHANG ; Meixia WANG ; Shuang MU
Chinese Medical Ethics 2017;30(6):737-741
Objective:This thesis aims to construct the evaluation index system ethics management in Beijing medical institutions for application research.Method:The author applied the evaluation system which was agreed and adjusted by expert groups in four medical and health institutions.Result:After three rounds of expert groups have reached the agreement,the Evaluation Index System on Human Research Ethics Management in Beijing Medi-cal Institutions is established,which contains 6 first-class indicators,16 second-class indicators and 39 third-class indicators.The application on four medical and health institutions show that the evaluation index system is practical,operable and is of high degree of distinction.Conclusion:The evaluation index system is scientific,rea-sonable,practical and is of high degree of validity,credibility and distinction.
5.Giant fibrothecoma arising in an accessory ovary of a pregnant woman.
Chun-Yan LI ; Shao QIAN ; Hui ZHANG ; Ming LIU ; Min SHI ; Yu-Lan MU ; Zi-Jiang CHEN
Chinese Medical Journal 2013;126(6):1195-1195
Adult
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Female
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Humans
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Ovarian Diseases
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diagnosis
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Ovarian Neoplasms
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diagnosis
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Pregnancy
6.Regulation of nitric oxide donor JS-K on tumor energy metabolism in H22 tumor-bearing mice
Ling LIU ; le Zi HUANG ; na Hui SHEN ; Jing WANG ; chen Mu LIU ; lin Chang HUA
Chinese Journal of Pharmacology and Toxicology 2017;31(7):730-735
OBJECTIVE To investigate the regulation of{O2 (2,4-dinitrophenyl)1-[(4-ethoxycarbonyl) piperazin-1-yl] diazen-1-ium-1, 2-diolate} (JS-K), a nitric oxide donor, on tumor energy metabolism in H22 tumor-bearing mice. METHODS The hepatoma animal model in BALB/c mice was established with H22 cell line. The inoculated mice were randomly divided into four groups. The JS-K group and model group received JS-K (0.75 and 1.50 mg?kg-1) and saline via tail the vein intravenously once every 3 d for 14 d, and 5 injections, respectively. The fluorouracil (5-FU) group received 5-FU 20 mg·kg-1 by intra-peritoneal injection once a day for 14 d. On the 15th day after the first administration, mice were sacri-ficed and the tumor, thymus and spleen were isolated and weighed immediately. The tumor growth inhibitory rate and organ index were calculated. The activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), succinate dehydrogenase (SDH), adenosinetriphosphatase (ATPase), and the levels of lactic acid (LD) and adenosine triphosphate (ATP) in tumor tissues were determined by colorimetric method. The expression of hypoxia-inducible factor 1 alpha (HIF-1α) and hexokinaseⅡ(HKⅡ) in the tumor tissue was analyzed by Western blotting. RESULTS Compared with model group, the tumor mass of JS-K 0.75 and 1.50 mg · kg-1 groups was significantly reduced (P<0.01), and the tumor growth inhibitory rate was 23.9%and 50.3%, respectively. There was no diffrence in thymic and splenic indexes between JS-K group and model group. The activity of HK, PFK, SDH, PK and ATPase of tumor tissue in model group was 22.6±3.7, 14.4±2.6, (10.5±2.6) U·g-1protein, (12.9±3.2) kU·g-1 protein and (0.70 ± 0.10) mmolPi · g-1protein · h-1, respectively, which dropped by 42.0%, 26.6%, 22.7%, 23.3%and 21.7%respectively (P<0.01, P<0.05) in JS-K 1.50 mg?kg-1 group. Compared with the model group, the level of ATP of tumor tissue in JS-K 1.50 mg?kg-1 groups dropped by 16.6%(P<0.01) and the level of LD in JS-K 0.75 and 1.50 mg?kg-1 groups dropped by 38.7%and 59.4%(P<0.01), respectively. In addi-tion, the expression of HIF-1αof tumor tissue in JS-K 1.50 mg?kg-1 group was decreased (P<0.01), and the expression of HKⅡ of tumor tissue in JS-K 0.75 and 1.50 mg?kg-1 groups was decreased signifi-cantly (P<0.05, P<0.01). CONCLUSION JS-K can inhibit the growth of tumor in H22 tumor-bearing mice and its mechanism may be related to regulating the tumor energy metabolism by inhibiting glycolysis and aerobic oxidation.
7.Case-control study on close reduction combined with minimally invasive percutaneous plate osteosynthesis for the treatment of distal fracture of tibial shaft.
Yin-Wen LIU ; Yu-Xin ZHENG ; Xue-Zong WANG ; Hu ZHANG ; Mu-Zheng SUN ; Wei XIAO-EN ; Gu XIN-FENG ; Yong KUANG ; Lei ZHANG ; Zi-Liang SHEN ; Hong-Sheng ZHAN ; Yin-Yu SHI
China Journal of Orthopaedics and Traumatology 2015;28(3):230-234
OBJECTIVETo compare the effects of close reduction combined with minimally invasive percutaneous plate osteosynthesis for distal fracture of tibial shaft.
METHODSFrom March 2009 to May 2013, there were 124 patients (89 males and 35 females, 30 of them were injured in a traffic accident and 94 were falling down in daily life, the ages ranging from 21 to 81 years old) who suffered from distal fracture of tibial shaft. Sixty-six patients (48 males and 18 females, 45 cases of type A, 12 cases of type B and 9 cases of type C) were treated with close manipulative reduction combined with minimally invasive percutaneous plate fixation. After close reduction, a minimal incision was made and a anatomic plate was inserted just along the medial tibia periostea, and then the fracture was fixed without fracture exposure. The other 58 patients (41 males and 17 females, 41 cases of type A, 10 cases of type B and 7 cases of type C) were treated with conventional open reduction and internal fixation. Length of the incision, operating time, early postoperative pain (recorded using the Visual Analog Scale score) and the outcome results (recorded using the evaluation standard of Johner-Wruhs) were compared.
RESULTSThe length of incision was meanly (7.34 ± 1.42) cm in MIPPO group and (21.82 ± 2.35) cm in ORIF group; operation time was (44.48 ± 10.00) min in MIPPO group and (59.42 ± 11.84) min in ORIF group. The postoperative radiographs verified good position of all screws and satisfactory bone fracture reduction in both groups. All the patients were followed up, and the duration ranged from 10 to 24 months (mean 15.2 months) in both groups. In MIPPO group,only one patient had delayed union and got union after Chinese herb therapy. The other 65 patients got bony union during 15 to 20 weeks. While in ORIF group, 3 patients suffered from nonunion and received reoperation with bone grafting, and 4 patients got bone infection.
CONCLUSIONTreatment of distal fracture of tibial shaft, combined with close reduction and MIPPO technique, has the advantages such as less invasion, less damage of blood supply, simplified procedure of operation and higher union rate, which is an ideal methods and is accordant to the biological demand.
Adult ; Aged ; Aged, 80 and over ; Bone Plates ; Case-Control Studies ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Male ; Manipulation, Orthopedic ; methods ; Middle Aged ; Tibial Fractures ; therapy
8.Investigation and Analysis of Current Situation of Human Research Ethical Management in Beijing Medical and Health Institutions
Zhengjuan HU ; Shuang MU ; Meixia WANG ; Xueqin WANG ; Mingjie ZI ; Zhuoran ZHANG ; Yingwei SUN ; Xiaolong MA ; Fang LIU ; Yiting LI
Chinese Medical Ethics 2018;31(2):230-235
Through the questionnaire survey on the current situation of human research ethical management in Beijing medical and health institutions, this paper analyzed the problems existing in ethical management of Beijing medical and health institutions and put forward corresponding countermeasures and suggestions. The results showed that overall status of human research ethical management in Beijing medical and health institutions was satisfying. But the ethical management levels of different types of medical and health institutions were quite different, and medical and health institutions, health family planning administration departments and academic teams should all make corresponding contributions.
9.Determination of two main components in bark of Paeonia suffruticosa by HPLC.
Yuan-zi TIAN ; Yan WANG ; Jian-zhen LIU ; Bei-bei YANG ; Jian-gong SHI ; Mu-zou WANG
China Journal of Chinese Materia Medica 2005;30(16):1265-1268
OBJECTIVETo establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.
METHODThe reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.
RESULTThe assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.
CONCLUSIONThe method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.
Acetophenones ; analysis ; Chromatography, High Pressure Liquid ; methods ; Gallic Acid ; analysis ; Paeonia ; chemistry ; Plant Bark ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control
10.Increased pretreatment levels of serum LDH and ALP as poor prognostic factors for nasopharyngeal carcinoma.
Guo LI ; Jin GAO ; Ya-Lan TAO ; Bing-Qing XU ; Zi-Wei TU ; Zhi-Gang LIU ; Mu-Sheng ZENG ; Yun-Fei XIA
Chinese Journal of Cancer 2012;31(4):197-206
Serum enzymes that play potential roles in tumor growth have recently been reported to have prognostic relevance in a diverse array of tumors. However, prognosis-related serum enzymes are rarely reported for nasopharyngeal carcinoma(NPC). To clarify whether the level of serum enzymes is linked to the prognosis of NPC, we reviewed the pretreatment data of lactate dehydrogenase(LDH), alkaline phosphatase (ALP), and glutamyl transferase (GGT) in 533 newly diagnosed NPC patients who underwent radical radiotherapy between May 2002 and October 2003 at Sun Yat-sen University Cancer Center. Patients were grouped according to the upper limit of normal values of LDH, ALP, and GGT. The Kaplan-Meier method and log-rank test were used for selecting prognostic factors from clinical characteristics and serum enzymes, and the chi-square test was applied to analyze the relationships of clinical characteristics and serum enzymes. Finally, a Cox proportional hazards model was used to identify the independent prognostic factors. We found that increased levels of LDH had poor effects on both overall survival and distant metastasis-free survival (P = 0.009 and 0.035, respectively), and increased pretreatment level of serum ALP had poor effects on both overall survival and local recurrence-free survival (P = 0.037 and 0.039, respectively). In multivariate analysis, increased LDH level was identified as an independent prognostic factor for overall survival. Therefore, we conclude that increased pretreatment serum LDH and ALP levels are poor prognostic factors for NPC.
Adolescent
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Adult
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Aged
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Alkaline Phosphatase
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blood
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Child
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Cisplatin
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administration & dosage
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Female
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Fluorouracil
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administration & dosage
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Humans
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Kaplan-Meier Estimate
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L-Lactate Dehydrogenase
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blood
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Male
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Middle Aged
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Nasopharyngeal Neoplasms
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blood
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drug therapy
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pathology
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radiotherapy
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Neoplasm Metastasis
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Neoplasm Recurrence, Local
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Prognosis
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Proportional Hazards Models
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Radiotherapy, Computer-Assisted
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Radiotherapy, Conformal
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Radiotherapy, Intensity-Modulated
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Survival Rate
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Young Adult
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gamma-Glutamyltransferase
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blood