1.Genetic polymorphism of DXS7423, DXS7424, DXS7132, DXS7133, DXS6804 loci on X-chromosome in Ewenke population.
Shan-zhi GU ; Mu-er TONG ; Xiao-zhong LI
Journal of Forensic Medicine 2005;21(3):192-194
OBJECTIVE:
To study the genetic polymorphism of five STR loci on X-chromosome in 98 Ewenke population of China and establish the related genetic database.
METHODS:
The sequence variation of DXS7423, DXS7424, DXS7132, DXS7133, DXS6804 loci on X-chromosome were analyzed by PCR following PAG electrophoresis and silver staining.
RESULTS:
The genetic data of the 5 X-chromosome STRs in Ewenke ethnic group of China is in accordance with Hard-Weinberg equilibrium.
CONCLUSION
Five X-STR loci could be used in forensic identification and population genetic research.
Chi-Square Distribution
;
China/ethnology*
;
Chromosomes, Human, X/genetics*
;
DNA/genetics*
;
Electrophoresis, Polyacrylamide Gel
;
Ethnicity/genetics*
;
Female
;
Forensic Medicine
;
Gene Frequency
;
Genetics, Population
;
Genotype
;
Humans
;
Male
;
Polymerase Chain Reaction
;
Polymorphism, Genetic/genetics*
;
Tandem Repeat Sequences
2.Genetic structure of X-STR loci of Ewenki nationality and its affinity with other nationalities.
Qiao-Fang HOU ; Xiao-Zhong LI ; Mu-Er TONG ; Liao-Jiang RONG ; Ran ZHANG ; Xu-Jun WANG ; Sheng-Bin LI
Journal of Central South University(Medical Sciences) 2007;32(2):276-281
OBJECTIVE:
To determine the genetic diversity of X-TR loci, and to evaluate the genetic structure X chromosome's of Ewenki nationality and its affinity with other nationalities.
METHODS:
We chose 9 X-TR (DXS6804, DXS7133, DXS101, DXS6789, DXS6799, DXS7423, HPRTB, DXS8378, DXS7132) as genetic markers from 99 irrelative individules to determine the genetic diversity of Ewenki in Inner Mongolian. Cluster analysis and phylogenic trees was applied to show the genetic distance among the nationalities.
RESULTS:
We got 51 alleles in the studied population, with the frequency diverse between 0.0109 and 0.6863. Genotype frequency was from 0.0217 to 0.3778. Heterozygosity(H),the power of discrimination(PD) and the polymorphism information conten (PIC) were consistent with the forensic application. Cluster analysis and phylogenic trees revealed that Ewenki nationality had estrangement genetic affinity with the other 3 major nationalities in inner mongolia and Han nationality in Xi'an.
CONCLUSION
The genetic information demonstrates that the 9 chosen gene makers were highly informative loci and are suitable for population genetics research and forensic application.
China
;
Chromosomes, Human, X
;
genetics
;
Cluster Analysis
;
Ethnic Groups
;
classification
;
genetics
;
Female
;
Gene Frequency
;
Genetic Markers
;
genetics
;
Genetic Variation
;
Genotype
;
Humans
;
Male
;
Microsatellite Repeats
;
genetics
;
Phylogeny
;
Polymorphism, Genetic
3.Genetic variation of 9 X-linked short tandem repeat loci among four populations of Inner Mongolian.
Xiao-zhong LI ; Mu-er TONG ; La OU ; Ran ZHANG ; Liao-jiang RONG ; Qiao-fang HOU ; Bin YU ; Sheng-bin LI
Chinese Journal of Medical Genetics 2008;25(1):89-92
OBJECTIVETo investigate the genetic structure of X chromosome in Mongolia, Ewenki, Elunchun and Dawoer in Inner Mongolia.
METHODSNine short tandem repeat (STR) markers on the X chromosome (DXS6789, DXS101, DXS8378, DXS7132, DXS7133, DXS7423, DXS6804, DXS6799 and HPRTB) were analyzed in the four populations from Inner Mongolian (Mongol, Ewenki,Oroqen and Daur) for their genetic diversity, forensic suitability and possible genetic affinities of the populations. Frequencies and other parameters of forensic interest were computed.
RESULTSThe results revealed that the nine markers described here have a moderate degree of variability in the population groups. And there are significant differences in the genetic variability among the populations. Genetic distance and cluster analyses show very low genetic distance between Mongol and Han (Xi'an) communities. The results based on genetic distance analyses are consistent with earlier studies based on linguistic as well as immigration history and origin of these populations.
CONCLUSIONThe nine STR loci studied here were found not only useful in studying genetic variations between populations but also suitable for human identity testing.
China ; ethnology ; Chromosomes, Human, X ; genetics ; Cluster Analysis ; Ethnic Groups ; genetics ; Female ; Genetic Variation ; Humans ; Male ; Microsatellite Repeats ; genetics