Objective To study the clinical significance of partial splenic embolization (PSE) for the treatment of hypersplenism and the relationship between the therapeutic effect and the area of embolization. Methods Sixteen patients with hypersplenism were divided randomly into two groups: Group 1 (n=5) and Group 2 (n=11). The embolization area in Group 1 was 40%-59% and that in Group 2 was 60%-80%. A follow-up for 1 year was conducted in all patients. Results The short-term effective rates (CR+PR) of thrombocyte count were 81.3% in Group 1 and 93.8% in Group 2, respectively, whereas the long-term effective rates were 62.5% and 87.5%, respectively, and the corresponding one-year recurrence rates were 23.1% and 6.7%. The long-term effective rates of WBC count were 71.4% and 82.2% in groups 1 and 2, respectively, and the one-year recurrence rates were 28.6% and 17.8%, respectively. The long-term effective rates of RBC count were 70% in Group 1 and 83.3% in Group 2, respectively, and the one-year recurrence rates were 22.2% and 11.8 %, respectively. Conclusion PSE, resulting in a low recurrence rate, is effective in treating hypersplenism.

Objective To investigate the prevention and treatment of the complications due to CT-guided percutaneous transthoracic biopsy. Methods CT-guided percutaneous transthoracic needle biopsy was performed in 168 patients aged from 14 to 79 years old. The complications and the contributing factors were analyzed. The methods for the prevention and treatment of the complicaitons were suggested. Results CT-guided percutaneous transthoracic needle biopsy was succeeded in 168 cases. The major complications associated with CT-guided needle biopsy included pneumothorax (8.33%) and pulmonary hemorrhage (20.24%). Pneumothorax was associated with the lesion size, lesion location, and times of puncture. Pulmonary hemorrhage was associated with the lesion size as well as the pre-operative evaluation of the vascular structures with contrast CT. Chest tube placement was performed in 1 out of 14 cases of pneumothorax. Other cases were not particularly treated. Conclusion The prevention of complications is important for CT-guided needle biopsy. The key factors to prevention the complications include accurate location of the lesions and correct evaluation of the vascular structures before the operation and skillful intraoperative puncture.

Purpose:The current study analyzed the polymorp hisms of DNA repair gene XRCC1 and relationship between genetic variations and s usceptibility to lung cancer Methods:A case-control study of 111 patients with lung cancer a nd 210 normal residents as controls was conducted to detect XRCC1 polymorphisms at Arg399Gln loci . Genotypes were analyzed by PCR-based restriction fragment le ngth polymorphism(RLFP) techniques. Results:XRCC1 codon 399 heterozygous genotype Arg/Gln might hav e weaker protection effect on squamous cell carcinoma and reduce the risk of lun g cancer on smokers. Homozygous genotype Gln/Gln of XRCC1 codon 399 might increa se the risk of lung cancer and have synergistic effect with smoking. Conclusions:The results demonstrated that genetic polymorphism of XRCC1 DNA repair gene might contribute to the susceptibility to lung cancer a nd have synergistic effect with smoking.

BacKground:Human pancreatic cancer is a highIy maIignant tumor of digestive system. CurrentIy,gemcitabine based conventionaI chemotherapy has onIy very Iimited efficacy on metastasis of pancreatic cancer. Studies have shown that thymoquinone has remarkabIe effect of inhibiting proIiferation and enhancing apoptosis on a variety of cancer ceIIs. Aims:To investigate the effect and mechanism of thymoquinone on inhibiting the migration and invasion of human pancreatic cancer BxPC-3 ceIIs in vitro. Methods:Human pancreatic cancer BxPC-3 ceIIs were conventionaIIy cuItured and treated with different concentrations of thymoquinone. The migration and invasion of BxPC-3 ceIIs were determined by Boyden chamber assay. The expressions of FAK,Akt and phosphoryIation of Akt were measured by Western bIotting,and immunofIuorescence was used to detect expression of FAK,focaI adhesions and F-actin. Results:The inhibitory rates of 10,25μmoI/L thymoquinone on migration of BxPC-3 ceIIs were 43. 4% and 73. 8%,respectiveIy,and the inhibitory rates of invasion were 60. 5% and 75. 6%,respectiveIy. The reduction of migration and invasion of pancreatic cancer BxPC-3 ceIIs by thymoquinone was in a dose-dependent manner( P < 0. 05 ). Thymoquinone obviousIy down-reguIated the expression of FAK and suppressed the phosphoryIation of Akt in BxPC-3 ceIIs. Thymoquinone induced the dispersed distribution of FAK in cytopIasm and inhibited the formation of focaI adhesions and assembIy of F-actin. Conclusions:Thymoquinone inhibits the migration and invasion of human pancreatic cancer BxPC-3 ceIIs in a dose-dependent manner in vitro through suppression of FAK/PI3K/Akt signaIing pathway and activity of kinase.

ObjectiveTo evaluate the impacts of pereutaneous nephrolithotomy (PCNL) on perioperative renal hemodynamics.MethodsThe hemodynamics of operated renal arteries of 30 patients who underwent unilateral PCNL with single pole access were observed 1 day before and 5-7 days after operation with CDFI.Parameters were analyzed statistically.ResultsAfter operation,resistance index (RI) of renal arteries decreased (P＜0.05).The diastolic flow statistically increased at main renal artery (MRA) of renal hilus,interlobar renal artery and interlobular renal artery (all P＜0.05).After PCNL,in serious hydronephrosis patients,RI decreased (P＜0.05) at segmental renal artery (SRA) and interlobar artery,end-diastolic flow velocity (Vmin) increased at interlobar renal artery (P＜0.05).In moderate hydronephrosis patients,RI decreased at all renal arteries (P＜0.05) after PCNL,Vmin increased at MRA and interlobular renal artery (P＜0.05).In minor hydronephrosis patients,RI decreased at MRA and SRA,Vmin increased at SRA.In patients without hydronephrosis,RI changeed like serious hydronephrosis patients.ConclusionAfter PCNL,ipsilateral renal perfusion improves,renal diastolic flow increases and RI decreases.CDFI can be used to observe the blood perfusion of kidney,and provide quantitative information of renal hemodynamics.

Objective To observe the in vivo degradation of poly-DL-lactic acid ureteral stent in canine and the pathological change in canine ureter.Methods Poly-DL-lactic acid ureteral stent was inserted into 19 canine ureters,respectively.The stents were taken out every two weeks with their degradation observed with electronic microscopy and their molecular weight change detected by gel permeation chromatography.Pathological change in ureteral tissue specimens stained with hematoxylin and eosin was observed by light microscopy.Results The molecular weight of poly-DL-lactic acid ureteral stent was decreased to 8 033 seven weeks after degradation from 68 900 before degradation,with a degradation rate of 88.3%.Electronic microscopy showed that the number of pores in stent was gradually increased and their diameter became larger.The stent was degraded.The main pathological change observed in canine ureter tissue was non-specific inflammation.Conclusion Poly-DL-lactic acid ureteral stent is good in degradation property and tissue compatibility in vivo.

Objective To study the effect of authorization theory on patients with new-diagnosed type 2 diabetes with impaired glucose regulation.Methods Authorization education on diabetes were carried out in 54 new-dhgnosed type 2 diabetes patients with impaired glucose regulation for 1 year.The blood glucose,body weight,BML,systolic and diastolic blood pressure,waist circumference,waist-hip ratio and adiponectin were examined before and after authorization education.Courses of education were divided into five steps:identify the problem;express feelings;Set goals;make a plan;evaluate the result.Results All the above factors alleviated after authorization education,P＜0.05.Conclusions Authorization education for patients can make them change their living style actively and achieve the goal of behavior change.

Objective To explore the clinical application of CT-guided automated needle biopsy in the diagnosis of pulmonary lesions. Methods CT-guided automated needle biopsy was performed on 168 patients in which the pulmonary lesions were not confirmed by clinical and imaging diagnoses. The success rate of puncture, accurate diagnosis rate, and complications were analyzed. Results The operation was performed successfully in all cases (100%). Accurate diagnosis was made in 158 out of 168 cases (94.05%), including 93 cases of lung cancer, 4 cases of metastatic lesions, 28 case of tuberculosis, and 33 cases of chronic inflammatory masses. The major complications due to the operation were pneumothorax (8.33%) and pulmonary hemorrhage (20.24%). Conclusion CT-guided percutaneous transthoracic automated needle biopsy, a feasible and accurate diagnostic method for the treatment of pulmonary lesions, and resulting in low occurrence of complications, can be used in clinical practice more widely.

Objective To investigate the role of RNA-binding protein La protein in the invasion and migration of cervical cancer cells.Methods RNAi technology was used to silence the La protein in HeLa cell,a cell line of cervical cancer,and then screened by G418.Finally the stably expressed HeLa-shLa cell line was constructed and then wound healing,Transwell,Western blot and gelatin zymography assay were performed. Results After La protein HeLa was silenced by RNAi,the invasion and migration capabilities of HeLa cells were decreased significantly compared with those of the controls.Meanwhile,SiRNA-mediated depletion of La reduced the expression of MMP-2 and increased the expression of TIMP-2.Meanwhile the activity of MMP-2 was reduced too.Conclusion RNA-binding protein La promotes the invasion and migration of cervical cancer cells,which may be related to regulating its matrix metalloproteinases and inhibitors.

Schistosomiasis is a kind of zoonosis with serious hazard,which is popular in many countries and regions in the world. One of the efforts for schistosomiasis prevent and control is developing new drugs and vaccines,and knowing the tran?scription regulation mechanism and the function of transcription factors will help us find the targets of new drugs and vaccines as soon as possible. This article reviews the progress of Schistosoma transcription factors and research methods.