1.The Expression of P53 and Phosphorylation of H2AX in Germ Cells of Varicocele Rats.
Won Jin CHO ; Moon Hwan YEO ; Ho Jin YOU ; In Youb CHANG ; Hyung Yoon MOON ; Chul Sung KIM
Korean Journal of Urology 2006;47(12):1354-1360
PURPOSE: To explore the expressions of P53 and phosphorylation-H2AX in varicocele-induced rat testes. MATERIALS AND METHODS: A total of 16 adult male Sprague-Dawley rats underwent an operation; 12 underwent an experimental varicocele and 4, as controls, were sham-operated. Groups of 4 varicocele-induced rats underwent a left orchiectomy after 2 or 3 weeks, or both orchiectomies after 4 weeks. The sham-operated rats underwent both orchiectomies after 4 weeks. Sections of both testes from each animal were studied. The changes in the expressions of P53 and phosphorylation of H2AX were determined using immunohistochemistry and western blot. RESULTS: Immunohistochemical staining of the left testes in the varicocele- induced rats showed that the expressions of P53 and phosphorylation of H2AX had not begun 2 weeks postoperatively, but remarkable results were observed after 3 and 4 weeks. Both testes of the varicocele-induced rats showed the expressions of P53 and phosphorylation of H2AX after 4 weeks, with the left testes being more distinctive in immunohistochemical staining compared to the right. Western blot of the left testes in the varicocele- induced rats also showed unclear expressions of P53 and gamma-H2AX after 2 weeks. Considerable distinction was seen after 3 and 4 weeks compared to the control group. CONCLUSIONS: Our results suggest that experimental varicocele is associated with increased sperm DNA damage. These changes may be related to abnormal spermatogenesis.
Adult
;
Animals
;
Blotting, Western
;
DNA Damage
;
Germ Cells*
;
Humans
;
Immunohistochemistry
;
Male
;
Orchiectomy
;
Phosphorylation*
;
Rats*
;
Rats, Sprague-Dawley
;
Spermatogenesis
;
Spermatozoa
;
Testis
;
Varicocele*
2.A Case of Lung Metastasis of Mesoblastic Nephroma in Adulthood.
Jin Wook MOON ; Kil Dong KIM ; Dong Hwan SHIN ; Chang Hoon HAHN ; Jae Ho JUNG ; Mu Suk PARK ; Sang Youn JUNG ; Jae Hyuk LEE ; Young Sam KIM ; Se Kyu KIM ; Sung Kyu KIM ; Joon CHANG
Tuberculosis and Respiratory Diseases 2003;55(4):402-407
Mesoblastic nephroma is a neoplasm of the kidney which is characterized by interlacing bundles of spindle mesenchymal cells. It is usually diagnosed during the first six months of life and is mostly benign. Incidence in adults is exceedingly rare. In most cases, only total excision is required without postoperative adjuvant therapy, and the rare cases of local recurrence have usually been related to incomplete removal. However, mesoblastic nephroma may behave aggressively, in contrast to a congenital mesoblastic nephroma. Several cases of metastatic mesoblastic nephroma have been previously described. We report herein a case of a 42-year-old woman with mesoblastic nephroma which recurred as a large metastatic lung mass seven years after the nephrectomy. The patient presented with chest wall discomfort for four days. Seven years previously, total nephrectomy had been performed because of a right renal tumor which had been diagnosed as a mesoblastic nephroma. There had been no evidence of recurrence for five years, after which she discontinued follow-up. On readmission two years later, chest X-ray and CT scan revealed a large lung mass in the left upper lobe. It was completely excised and the pathologic examination was identical with that of the original renal tumor. Synovial sarcoma was excluded because the fusion transcripts of the SYT-SSX fusion gene associated with the t(X;18) translocation were negative. The final diagnosis was a lung metastasis of mesoblastic nephroma and the patient remained free of disease for 7 months postoperatively.
Adult
;
Diagnosis
;
Female
;
Follow-Up Studies
;
Humans
;
Incidence
;
Kidney
;
Lung*
;
Neoplasm Metastasis*
;
Nephrectomy
;
Nephroma, Mesoblastic*
;
Recurrence
;
Sarcoma, Synovial
;
Thoracic Wall
;
Thorax
;
Tomography, X-Ray Computed
3.The Results of Nation-Wide Registry of Age-related Macular Degeneration in Korea.
Kyu Hyung PARK ; Su Jeong SONG ; Won Ki LEE ; Hee Sung YOON ; Hyoung Jun KOH ; Chul Gu KIM ; Yun Young KIM ; Se Woong KANG ; Ha Kyoung KIM ; Byung Ro LEE ; Dong Heun NAM ; Pill Young LEE ; Hyoung Su KIM ; Hyeoung Chan KIM ; Si Yeol KIM ; In Young KIM ; Gwang Su KIM ; Kuhl HUH ; Jae Ryung OH ; Shin Dong KIM ; Sang Jun LEE ; Young Duk KIM ; Soon Hyun KIM ; Tae Gon LEE ; Sung Won JOE ; Dong Cho LEE ; Mu Hwan CHANG ; Si Dong KIM ; Young Wok JOE ; Sun Ryang BAE ; Ju Eun LEE ; Boo Sup OUM ; Ill Han YOON ; Soon Ill KWON ; Jae Hoon KANG ; Jong In KIM ; Sang Woong MOON ; Hyeong Gon YU ; Young Hee YOON ; Gwang Yul CHANG ; Sung Chul LEE ; Sung Jin LEE ; Tae Kwan PARK ; Young Hun OHN ; Oh Woong KWON ; Woo Hyok CHANG ; Yeon Sik YANG ; Suk Jun LEE ; Su Young LEE ; Chang Ryong KIM ; Jung Hee LEE ; Yeon Sung MOON ; Jae Kyoun AHN ; Nam Chun JOE ; Gwang Ju CHOI ; Young Joon JOE ; Sang Kook KIM ; Sung Pyo PARK ; Woo Hoo NAM ; Hee Yoon JOE ; Jun Hyun KIM ; Hum CHUNG
Journal of the Korean Ophthalmological Society 2010;51(4):516-523
PURPOSE: To evaluate the incidence and clinical features of age-related macular degeneration (AMD) in Korea. METHODS: Web-based (www.armd-nova.or.kr) registration was conducted for AMD patients aged 50 or more who were newly diagnosed by retinal specialists in Korea from August 20, 2005 to August 20, 2006. Patient data including ophthalmologic examination, fundus photography, fluorescein angiogram and/or indocyanin green angiogram (ICG), past medical history, behavioral habit, combined systemic diseases were up-loaded. RESULTS: Among finally enrolled 1,141 newly diagnosed AMD patients, 690 patients (60.5%) were male and 451 patients (39.5%) were female. The average age of AMD patients was 69.7+/-8.0. Early AMD was observed in 190 patients and 951 patients had late AMD. Classic choroidal neovascular membrane (CNVM) was observed in 18.6% of exudative AMD patients and 63.4 % had occult CNVM. Subfoveal CNVM was observed in 80.4% of the patients with CNVM. Among the 580 exudative AMD eyes that performed indocyanin green angiography (ICG), 184 eyes (31.7%) had polypoidal choroidal vasculopathy (PCV) and 36 eyes (6.2%) showed retinal angiomatous proliferation (RAP). Age, male gender, smoking, diabetes and hypertension significantly increased the risk of the AMD among Koreans. CONCLUSIONS: Because of the low rate of participation by retinal specialists, definite incidence of AMD was not obtainable. However, the estimated 1-year AMD incidence in the Pusan area of Korea is at least 0.4%. In contrast to Western people, 31.7% of exudative AMD cases were revealed to be PCV and 6.2% were revealed to be RAP. This discrepancy between ethnic groups should be considered in the diagnosis and treatment modality selection of Korean AMD patients.
Aged
;
Angiography
;
Choroid
;
Ethnic Groups
;
Eye
;
Female
;
Fluorescein
;
Humans
;
Hypertension
;
Incidence
;
Korea
;
Macular Degeneration
;
Male
;
Membranes
;
Photography
;
Retinaldehyde
;
Smoke
;
Smoking
;
Specialization