Aim To study the effect of Bay k8644 on cardiac function with the model of myocardial ischemia-reperfusion injury(MIRI)in Rats.Methods Rat MIRI was induced by occluding the left anterior descending coronary artery for 40 min followed by 90 min reperfusion.Eighteen rats were divided randomly into 3 groups: saline,Bay k8644 and 0.1% dimethyl sulfoxide(DMSO)intravenously injected 30 min after ischemia.Heart functions were assessed before ischemia and 5,20 and 40 min after initiation of ischemia and 5,30,60 and 90 min after reperfusion,by measuring heart rate(HR),the left ventricular systolic pressure(LVSP),left ventricular end diastolic pressure(LVEDP) and ?dp/dt_(max).Results After ischemia,LVSP and +dp/dt_(max) decreased(P

Objective To study the antioxidant effects of casein peptides with different molecular weights on liver tissue in mice.Methods Forty-eight mice were divided into four groups,and fed ig casein peptides in different molecular weights,3ku and distilled water as control.Results Casein peptides could improve the antioxidation ability of the mice,as to increase glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities of liver tissue,and reduce malondialdehyde (MDA).The antioxidant enzymes activities after giving casein peptides for 30d and 15d were significantly different.Conclusions Casein peptides can enhance the antioxidation of the mice and especially more effect with the molecular weight

Objective To discuss the pressure level of nurses in Shenzhen special administrative region (SAR) and its influencing factors and adopt selective psychological intervention to alleviate their working pressure,increase working efficiency and nursing quality. Methods The investigation by questionnaires was carried out by reference of working stress scale in 250 nurses and the results were analyzed.Results The main sources of working pressure include being afraid of making mistakes during work,frquent shift-work, low wages and other benefits,the character and social status of nursing work,less chances of advanced study and continuing education,lower recognition by patients,family and society.The working pressure was different in nurses with different position,time length of nursing work and education background. Conclusion Great working pressure existed in nurses in Shenzhen special administrative region(SAR).It is suggested that the management department should lessen or eliminate nurses'working pressure sourcs.Besides, they should practice measures to alleviate nurses'working pressure according to selective conditions.

Objective To study the association between metastatic ratio of lymph node and clinical pathological features and its prognosis in elderly patients with gastric cancer.Methods The first postoperative pathological data of 219 patients with gastric cancer aged ≥ 60 years were retrospectively analyzed.The relationships of metastatic ratio of lymph node with gender,differentiation degree,depth of tumor invasion,vascular invasion,perineural invasion were analyzed.We used Kaplan-Meier survival analysis and Cox regression model to analyze this relationship.Results The metastasis rate of lymph node was higher in gastric cancer patients with higher degree of differentiation,deeper tumor-infiltration,vascular invasion or neural invasion,and the metastasis rate of lymph node was increased in females than in males (all P＜0.05).Kaplan-Meier survival analysis and Cox regression model analysis suggested that the metastasis rate of lymph node was an independent factor for postoperative survival rate in the elderly with gastric cancer.Conclusions The metastatic ratio of lymph node could be used to estimate the clinical prognosis of elderly gastric cancer.

Objective To explore the changes of Th1/Th2 cytokine in patients with Graves disease(GD) and the regulation effect of Shengmai Injection on its balance.Methods The patients with GD were randomly divided into 2 groups,the Shengmai treated group and the Methimazole treated group,30 cases per group and 30 healthy subjects were enrolled in this study.The serum concentrations of their Th1 cytokine(IFN-?) and Th2 cytokine(IL-10) were assayed by ELISA before and after treatment.Results The serum levels of INF-? and IL-10 in patients with GD were significant higher and the ratio of INF-?/IL-10 were significant lower than healthy subjects(P

AIM To examine antithrombotic effects of arecoline on the arterial thrombosis induced by carrageenin in mice through modulating the functions of endothelium and determine its mechanisms from hemostatic system, the platelet aggregative functions and the bioactive factors released by vascular endothelial cells. METHODS Kappa carrageenin was given ip in mice and mice were fed at the temperature of 20 to 21 degrees and at the humidity of 30 percent to 50 percent. RESULTS On the foregoing models of thrombosis, arecoline could antagonize the formation of thrombosis through activating the endothelial target for acetylcholine in a dose dependent manner and its antithrombotic potency was 250 to 500 times greater than aspirin; while under the same conditions, pilocarpine could not antagonize the formation of thrombosis. The levels of TT, PT, KPTT and MAR had no prominent changes compaired with control groups. The levels of t-PA became higher greatly than normal and the levels of PAI 1 became lower greatly than normal 2 hours after intravenous injection of arecoline in rats. Arecoline could decrease the higher plasma levels of thromboxane A2 and increase the lower plasma levels of prostacyclin in a dose dependent manner in the mice tail thrombosis induced by carrageenin. CONCLUSION The antithrombotic effects of arecoline are associated with activating the endothelial target for acetylcholine closely, but are not associated with muscarinic receptors,and not relevant to hemostatic systems or functions of platelet aggregation directly.

The environmental pollution caused by organo tin attracted much concern,at the same time, the methodological study on determination of organo tin in environment made a desirable development, the chromatograph technique was used widely.A more detailed review on application of gas chromatograph and its coupled techniques in the environment organo tin compounds analysis was presented in this paper.

Objective:To explore a good method which might be used to reconstruct the nasal deformity after unilateral complete cleft lip has been repaired. Methods: After being folded into a small column, the conchal cartilage was transplanted into nasal columella to reconstruct the frame of the nasal columella so that the nasal tip was supported strongly. Then the bilateral columella cartilages were raised to its normal and symmetrical position and then sutured to the conchal cartilage column. After that, a "Z" plasty was made on the ridge of nasal mucosa under nostril on the side of the cleft. Results: A total of 44 cases were treated by the method above, and above 90% of cases were evaluated as satisfactory. Conclusion:It is a good method to reconstruct the nasal deformity by transplanting conchal cartilage into nasal columella.

Red cell isoenzymes EsD and PGM and serum Hp are of genetic polymorphism which is inheritedsccording to the Law of Meqdelian Segregation. The distribution of their gene frequencies varies withraces. In this paper,the phenotype frequencies of the EsD,PGM and Hp have been investigated by theemthod of mixed starch/agarose gel electrophoresis and polyacrylamide gel electrophoresis among fivenationalities in Inner Mongolia, namely Creqen, Ewenki, Dawuor, Northeast Mongolian and Buryat.The gene Oroqen,Ewenki,Dawuor,Northeast Mongolian and Buryat. The gene frequencies were alsocalculated. The phenotype frequencies of EsD,PGM and Hp are compared not only among the five nationalities but with those reported by other countries.

Objective To evaluate the efficacy and safety of 0.75% ropivacaine versus 0.75% bupivicaine for combined spinal and epidural anesthesia(CSEA) for cesarean section. Methods Forty primiparae(ASA Ⅰ-Ⅱ) scheduled for elective cesarean section were divided into two groups: ropivacaine group received 0.75% ropivacaine 1 .2-1 . 4ml(9-10. 5mg) for spinal anesthesia and bupivicaine group received same amount of 0.75% bupivicaine. During operation when spinal analgesia was inadequate, 1.6% lidocaine was supplemented via epidural catheter. Blood pressure , heart rate and SpO2 were monitored. Sensory block(pin prick), motor block(modified Bromage scale), quality of analgesia and relaxation of abdominal wall were assessed. Apgar score of the neonates and umbilical artery blood gas as well as side effects were recorded. Results There was no significant difference between the two groups in age, height, body weight of the patients and duration of operation. The height of block was comparable between the two groups but the onset time was longer and duration of block was shorter in ropivacaine group. Analgesia and muscle relaxation were satisfactory and Apger score was 10 at 1 mm and 5mm in both groups. Blood gas values were within normal range in both groups. Motor block was weaker with ropivacaine than that with bupivacaine(P