1.Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces.
A Dilek NALBANT ; Ayse KALKANCI ; Banu FILIZ ; Semra KUSTIMUR
Yonsei Medical Journal 2008;49(4):647-654
PURPOSE: The aim of this study is to examine the effect Klorhex and Fittydent, which are used as cleaning agents on the adhesion of Candida on the surfaces of acrylic denture and palatal mucosa. In addition, ability of yeasts to adhere to acrylic strips was evaluated after applying these agents in vitro. MATERIALS and METHODS: Each group of 15 patients cleaned their dentures with either Klorhex or with Fittydent. The control group cleaned their dentures with water. RESULTS: It was found that 62.2% of the patients had colonies of Candida species on their palatal mucosa which was reduced to 51.1% after using these cleaning agents. The colonization rate with Candida spp on their dentures was reduces from 82.2% to 68.8% using these cleaning agents. The mean adhesion value of the Candida strains isolated from the acrylic strips were found to be 75cell/strip prior to applying the Klorhex and Fittydent and 37.5cell/strip and 15cell/strip after applying these agents, respectively. CONCLUSION: These results showed that Klorhex and Fittydent have a certain preventive effect on the colonization rate of Candida spp on the surface of these dentures, the palatal mucosa, as well as on the acrylic strips in vitro.
*Acrylates
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Adhesiveness/drug effects
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Antifungal Agents/*pharmacology
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Candida/*cytology/*drug effects
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Cell Adhesion/drug effects
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Denture Cleansers/*pharmacology
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*Dentures
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Mouth Mucosa/drug effects/microbiology
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Polyvinyls/pharmacology
2.Inhibitory effect of baicalin on germ tube formation and adhesion of Candida albicans.
Changzhong WANG ; Xin FENG ; Xiaolu ZHANG ; Qian ZHU ; Chuanqi XIE ; Huijuan CHENG ; Yan WANG ; Yun YUN
China Journal of Chinese Materia Medica 2010;35(23):3216-3218
OBJECTIVETo investigate the effects of baicalin against Candida albicans germ tube formation, and adherence to buccal epitherial and vaginal epitherial cells.
METHODVarious concentrations of baicalin (100, 50, 10 mg x L(-1)) were incubated with C. albicans suspension, the mixed suspension of C. albicans and human buccal epitherial cells, the mixed suspension of C. albicans and vaginal epitherial cells, respectively. The effects of baicalin on C. albicans germ tube formation, and adherence to buccal epitherial and vaginal epitherial cells were then assessed microscopically.
RESULTAll concentrations of baicalin could inhibit C. albicans germ tube formation, and adherent to buccal epitherial and vaginal epitherial cells,while there was no significant difference between standard and clinical strains.
CONCLUSIONBaicalin could inhibit C. albicans germ tube formation, and adherence to buccal epitherial and vaginal epitherial cells.
Adult ; Anti-Infective Agents ; pharmacology ; Candida albicans ; drug effects ; growth & development ; physiology ; Candidiasis ; drug therapy ; microbiology ; Cheek ; microbiology ; Epithelial Cells ; microbiology ; Female ; Flavonoids ; pharmacology ; Humans ; Mouth Mucosa ; microbiology ; Vagina ; microbiology ; Young Adult
3.Effect of fibrinogen on the adherence of Porphyromonas gingivalis to human oral epithelial cells.
Song GE ; Ya-fei WU ; Tian-jia LIU ; Lei ZHAO ; Xiao-lu FEI
Chinese Journal of Stomatology 2006;41(2):69-73
OBJECTIVETo study the role of fibrinogen molecule in the pathogenesis of periodontal diseases.
METHODSAn in vitro cell culture model was used. Methyl-(3)H Thymidine radiolabeled Porphyromonas gingivalis (Pg) ATCC 33277 were examined for their ability to adhere to and invade the confluent monolayers of human oral epithelial KB cells with or without exogenous human fibrinogens by scintillation spectrometry.
RESULTSThe addition of exogenous fibrinogens made more amount of and higher ratios of adhesive and invasive Pg, in contrast to the group without exogenous fibrinogen (P < 0.001). At different concentrations of exogenous fibrinogen, the amount and ratios of adhesive and invasive Pg varied significantly (P < or = 0.007). The higher concentrations of exogenous fibrinogen was added, the greater amount and ratios of adhesive and invasive Pg were found.
CONCLUSIONSFibrinogen promotes the adherence of Pg to human oral epithelial cells and may play an important role in the pathogenesis of periodontal diseases.
Bacterial Adhesion ; drug effects ; Fibrinogen ; administration & dosage ; pharmacology ; Humans ; KB Cells ; Mouth Mucosa ; drug effects ; microbiology ; Periodontitis ; etiology ; Porphyromonas gingivalis ; pathogenicity
4.Oral mucosal lipids are antibacterial against Porphyromonas gingivalis, induce ultrastructural damage, and alter bacterial lipid and protein compositions.
Carol L FISCHER ; Katherine S WALTERS ; David R DRAKE ; Deborah V DAWSON ; Derek R BLANCHETTE ; Kim A BROGDEN ; Philip W WERTZ
International Journal of Oral Science 2013;5(3):130-140
Oral mucosal and salivary lipids exhibit potent antimicrobial activity for a variety of Gram-positive and Gram-negative bacteria; however, little is known about their spectrum of antimicrobial activity or mechanisms of action against oral bacteria. In this study, we examine the activity of two fatty acids and three sphingoid bases against Porphyromonas gingivalis, an important colonizer of the oral cavity implicated in periodontitis. Minimal inhibitory concentrations, minimal bactericidal concentrations, and kill kinetics revealed variable, but potent, activity of oral mucosal and salivary lipids against P. gingivalis, indicating that lipid structure may be an important determinant in lipid mechanisms of activity against bacteria, although specific components of bacterial membranes are also likely important. Electron micrographs showed ultrastructural damage induced by sapienic acid and phytosphingosine and confirmed disruption of the bacterial plasma membrane. This information, coupled with the association of treatment lipids with P. gingivalis lipids revealed via thin layer chromatography, suggests that the plasma membrane is a likely target of lipid antibacterial activity. Utilizing a combination of two-dimensional in-gel electrophoresis and Western blot followed by mass spectroscopy and N-terminus degradation sequencing we also show that treatment with sapienic acid induces upregulation of a set of proteins comprising a unique P. gingivalis stress response, including proteins important in fatty acid biosynthesis, metabolism and energy production, protein processing, cell adhesion and virulence. Prophylactic or therapeutic lipid treatments may be beneficial for intervention of infection by supplementing the natural immune function of endogenous lipids on mucosal surfaces.
Anti-Bacterial Agents
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pharmacology
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Bacterial Proteins
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drug effects
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Colony Count, Microbial
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Fatty Acids
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pharmacology
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Humans
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Lipids
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pharmacology
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Microscopy, Electron
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Mouth Mucosa
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chemistry
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immunology
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microbiology
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Porphyromonas gingivalis
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chemistry
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drug effects
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ultrastructure
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Saliva
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chemistry
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microbiology
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Sphingolipids
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pharmacology
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Virulence
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drug effects