Objective To survey the point prevalence of healthcare-associated infection(HAI) in 13 tertiary hospitals in Dongguan,understand the occurrence of HAI in tertiary hospitals,so as to provide guidance for the prevention and control of HAI.Methods Through bedside investigation and medical record reviewing,HAI among inpatients in 13 tertiary hospitals in Dongguan at 0:00-24:00 of September 24,2014 were investigated.Results A total of 11 344 patients were investigated,379 patients developed 404 times of HAI,HAI rate was 3.34％,HAI case rate was 3.56％.Incidence of HAI in the intensive care unit was highest (12.82％).The main infection site was respiratory tract(n =173,42.48％).The usage rate of antimicrobial agents was 30.50％,most were for therapeutic use (including therapeutic+ prophylaxis) (n =2 545,73.55 ％)and single use (n =2 689,77.72％),pathogenic microorganism detection of specimens from patients receiving therapeutic antimicrobial use was 53.79％.A total of 299 strains of pathogenic orgamisms were detected from infected patients,the major were Escherichia coli (n =56,18.73 ％),Pseudomonas aeruginosa (n =54,18.06 ％),and Klebsiella pneumoniae (n =35,11.71 ％),92 strains of multidrug resistant organisms(MDROs) were isolated,accounting for 30.77％ of total isolated pathogens.Conclusion The basic characteristics of HAI in tertiary hospitals in this city were preliminarily investigated,identification of high risk departments,high risk population,and high risk links should be strengthened,HAI prevention and control measures should be implemented.

Objective To survey the resistance profile of clinical isolates to antibiotics across the hospitals in Dongguan,Guangdong Province during 2015.Methods Kirby-Bauer method or automated system was used to test the susceptibility of clinical isolates to selected antimicrobial agents.Results were analyzed according to CLSI 2015 breakpoints.The susceptibility data were analyzed using WHONET 5.6 software.Results A total of 29 665 strains of microorganisms were isolated,of which gram positive cocci accounted for 32.1％ (9 509/29 665) and gram negative bacilli accounted for 67.9％ (20 156/29 665),respectively.The prevalence of methicillinresistant Staphylococcus was 23.3％ (705/3 024) in S.aureus and 43.6％ (1 054/2 419) in coagulase-negative Staphylococcus.No vancomycin-resistant staphylococcal strain was found.ESBLs-producing strains accounted for 36.4％ (2 554/7 020) in E.coli and 24.5％(792/3 227) in Klebsiella isolates.The prevalence of carbapenem-resistant Enterobacteriaceae was 0.2％ (30/13 077).The prevalence of carbapenem-resistant Pseudomonas aeruginosa (CRPA) and carbapenem-resistant Acinetobacter baumannii (CRAB) was 16.0％ (500/3 116) and 53.9％ (827/1 533),respectively.The prevalence of penicillin-resistant S.pneumoniae (PRSP) strains was 10.1％ (142/1 404).Beta-lactamase was produced in 30.6％ (276/902) of the H.influenzae strains.The prevalence of vancomycin-resistant Enterococcus (VRE) strains was 0.7％ (10/1 441).Conclusions Periodic surveillance of antimicrobial resistance is valuable for rational antimicrobial therapy,formulation of treatment guidelines and infection control and prevention measures,as well as preventing the spread of drug-resistant strains.