Akt plays pivotal roles in many physiological responses including growth, proliferation, survival, metabolism, and migration. In the current studies, we have evaluated the isoform-specific role of akt in lysophosphatidic acid (LPA)-induced cell migration. Ascites from ovarian cancer patients (AOCP) induced mouse embryo fibroblast (MEF) cell migration in a dose-dependent manner. On the other hand, ascites from liver cirrhosis patients (ALCP) did not induce MEF cell migration. AOCP-induced MEF cell migration was completely blocked by pre-treatment of cells with LPA receptor antagonist, Ki16425. Both LPA- and AOCP-induced MEF cell migration was completely attenuated by PI3K inhibitor, LY294002. Furthermore, cells lacking Akt1 displayed defect in LPA-induced cell migration. Re-expression of Akt1 in DKO (Akt1(-/-)Akt2(-/-)) cells restored LPA-induced cell migration, whereas re-expression of Akt2 in DKO cells could not restore the LPA-induced cell migration. Finally, Akt1 was selectively phosphorylated by LPA and AOCP stimulation. These results suggest that LPA is a major factor responsible for AOCP-induced cell migration and signaling specificity of Akt1 may dictate LPA-induced cell migration.
1-Phosphatidylinositol 3-Kinase/physiology ; Adult ; Aged ; Animals ; Ascites/pathology ; Cell Movement/*drug effects ; Cells, Cultured ; Embryo, Mammalian ; Enzyme Activation/drug effects ; Female ; Humans ; Liver Cirrhosis/pathology ; Lysophospholipids/isolation & purification/*pharmacology ; Mice ; Middle Aged ; Ovarian Neoplasms/pathology ; Pregnancy ; Proto-Oncogene Proteins c-akt/*agonists/*metabolism ; Substrate Specificity