1.Tissue-engineered reconstitution of oral mucosa using polydioxanone mesh.
Seon Jae MOON ; So Yeon JOO ; Jin KIM ; Hak Yong KIM ; Jung Keug PARK ; In Ho CHA
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2003;29(4):249-256
The lack of sufficient oral mucosa available for intra-oral reconstruction has been dealt with by the use of skin or oral mucosa grafts harvested from donor sites but grafts requires more than one surgical procedures and could cause donor site morbidity. Many investigators have attempted to increase available soft tissue by tissue engineered skin or oral mucosa replacements for clinical applications. But, reconstructed mucosa by several methods have low physical properties such as rolling and contraction. The aims of this study were to develope an in vitro experimental model that maintains an epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally cultured oral mucosa embedded with Polydioxanone mesh by histological and immunohistochemical analysis. The results were as follows; 1. Oral mucosa reconstructed by three-dimensional organotypic culture revealed similar morphologic characteristics to equvalent normal oral mucosa in the point that they show stratification and differentiation. 2. The expression of cytokeratin 10/13 and involucrin in the cultured tissue showed the same pattern with normal oral mucosa suggesting that organotypic co-culture condition is able to induce cellular differentiation. 3. After insertion of polydioxanone mesh, increased tensile strength were observed. These results suggest that three-dimensional organotypic co-culture of the oral mucosa cell lines with the dermal equvalent consisting type I collagen and fibroblasts reproduce the morphologic and immunohistochemical characteristics similar to those in vivo condition. And increased physical properties by use of polydioxanone mesh will helpful for clinical applications.
Cell Line
;
Coculture Techniques
;
Collagen Type I
;
Fibroblasts
;
Humans
;
Keratins
;
Models, Theoretical
;
Mouth Mucosa*
;
Mucous Membrane
;
Polydioxanone*
;
Research Personnel
;
Skin
;
Tensile Strength
;
Tissue Donors
;
Transplants
2.Clinical evaluation of lung cancer in patients younger than 40 years.
Jun Ho MOON ; Kyoung Tae CHA ; Yong HUR ; Wook Su AHN ; Byung Yul KIM ; Jung Ho LEE
The Korean Journal of Thoracic and Cardiovascular Surgery 1993;26(11):861-865
No abstract available.
Humans
;
Lung Neoplasms*
;
Lung*
3.Two Cases of Angiodysplasia in Small Bowel : Intra - operative Endoscopic Transillumination Technique.
Jong Seo LEE ; Chang Ryeol CHA ; Won Il CHO ; In Sung MOON ; Sang Yong CHOO ; In Sik CHUNG ; Kyo Young LEE
Korean Journal of Gastrointestinal Endoscopy 1993;13(4):707-711
Angiodysplasia of small bowel is uncommon and frequently undiagnosed and presents a taxing surgical problem. It is usually diagnosed for unexplained gastrointestinal bleeding. For the surgeon, the main technical problem is that the lesion is impalpable, and invisible to the naked eye, so it usually cannot be identified unless bleeds actively at the time of surgery. Arteriography gives a little information about wax and wane pattern of bleeding in the lesion. Endoscopy is often unfruitful because the majority of lesions are submucosal and rarely exceed a few millimeters in diameter. Transillumination of the intestinal wall from inside of the lumen to the outside in a dark room can define the precise vascular anatomy of the wall. The delicate lesion of the angiodysplasia can be identified by this transillumination method. We described a simple intraoperstive endoscopic translllumination technique used successfully to identify an angiodysplasia in the small bovwel prior to the bowel resecion. This report summarized our experience and review of literature.
Angiodysplasia*
;
Angiography
;
Endoscopy
;
Hemorrhage
;
Taxes
;
Transillumination*
4.Treatment of tibial shaft fractures using unreamed intramedullary nailing.
Pjil Hyun CHUNG ; Moon Jib YOO ; Suk KANG ; Eung Nam CHA ; Yong Min KIM ; Jong Won KIM ; Hyung Ho OH
The Journal of the Korean Orthopaedic Association 1993;28(5):1725-1735
No abstract available.
Fracture Fixation, Intramedullary*
5.Application of Rapid Prototyping Technique and Intraoperative Navigation System for the Repair and Reconstruction of Orbital Wall Fractures.
Jong Hyun CHA ; Yong Hae LEE ; Wan Chul RUY ; Young ROE ; Myung Ho MOON ; Sung Gyun JUNG
Archives of Craniofacial Surgery 2016;17(3):146-153
BACKGROUND: Restoring the orbital cavity in large blow out fractures is a challenge for surgeons due to the anatomical complexity. This study evaluated the clinical outcomes and orbital volume after orbital wall fracture repair using a rapid prototyping (RP) technique and intraoperative navigation system. METHODS: This prospective study was conducted on the medical records and radiology records of 12 patients who had undergone a unilateral blow out fracture reconstruction using a RP technique and an intraoperative navigation system from November 2014 to March 2015. The surgical results were assessed by an ophthalmic examination and a comparison of the preoperative and postoperative orbital volume ratio (OVR) values. RESULTS: All patients had a successful treatment outcome without complications. Volumetric analysis revealed a significant decrease in the mean OVR from 1.0952±0.0662 (ranging from 0.9917 to 1.2509) preoperatively to 0.9942±0.0427 (ranging from 0.9394 to 1.0680) postoperatively. CONCLUSION: The application of a RP technique for the repair of orbital wall fractures is a useful tool that may help improve the clinical outcomes by understanding the individual anatomy, determining the operability, and restoring the orbital cavity volume through optimal implant positioning along with an intraoperative navigation system.
Computer Simulation
;
Humans
;
Medical Records
;
Orbit*
;
Orbital Fractures
;
Prospective Studies
;
Surgeons
;
Treatment Outcome
6.A Study on Air Pollution and Thermal Factors in Underground Shopping Center of Pusan Area.
Sung Yong CHOI ; Deog Hwan MOON ; Jong Tae LEE ; In Hyuk SONG ; Cha Eun LEE ; Sung Min LEE
Korean Journal of Preventive Medicine 1994;27(3):505-516
For the purpose of preparing the fundamental data on air pollution in underground shopping center and also contributing to the health improvement of resident, the authors measured the level of SO2, NO2, TSP, CO, CO2 and also some related factors as air temperature, air movement, relative humidity and mean radiation temperature at inside and outside of underground shopping center in Pusan from January to February and from July to August 1994. The results were as follows: 1. The mean concentration of CO within the underground shopping center was 3.1+/-1.3ppm in winter and 2.1+/-0.9 ppm in summer. There was a negative correlation (p<0.01) between inner CO concentration and temperature in summer and no correlation between inner CO concentration and outer CO concentration in underground shopping center 2. The mean concentration of C02 within the underground shopping center was 876+/-353 ppm in winter and 757+/-125 ppm in summer. There was a negative correlation (P<0.01) between inner CO2 concentration and air movement in summer and positive correlation (p<0.05) between inner CO2 oncentration and outer CO2 concentration in underground shopping center. 3. The mean concentration SO2 within a underground shopping center was 0.036+/-0.019ppm in winter and 0.040+/-0.013ppm in summer. There was a positive correlation(p<0.01) between inner SO2 concentration and positive correlation between inner SO2 concentration and outer SO2 concentration in summer and winter in Underground shopping center. 4. The mean concentration of NO2 within a underground shopping center was 0.052+/-0.038ppm in winter and 0.042+/-0.016ppm in summer. There was a no correlation between inner NO2 concentration and thermal factors in summer and winter and low correlation between inner NO2 concentration in underground shopping center. 5. The mean concentration of TSP within a underground shopping center was 430+/-214 microgram/m3 in winter, 366+/-73 microgram/m3 in summer, and very in excess of the atmospheric environmental quality standards of Korea(150 microgram/m3). There was low correlation between inner TSP concentration and temperature in summer and high correlation between inner TSP concentration and outer TSP concentration in underground shopping center.
Air Movements
;
Air Pollution*
;
Busan*
;
Humidity
7.Clinical analysis of completion pneumonectomy: Report of 11 cases.
Yong HUR ; Jae Hong PARK ; Joon Ho MOON ; Kyong Tae CHA ; Wook Soo AHN ; Byung Yul KIM ; Jung Ho LEE ; Hoe Sung YU
The Korean Journal of Thoracic and Cardiovascular Surgery 1993;26(11):851-855
No abstract available.
Pneumonectomy*
8.Measles Hepatitis in Children.
Sang Gil AN ; Ji Eun WUON ; Ki Moon CHA ; Yong Min CHUNG ; Hee Sup KIM ; Hann TCHAH ; Ho Jin PARK
Journal of the Korean Pediatric Society 1996;39(10):1371-1377
PURPOSE: Measles infection in children has been known to have many complications including hepatitis. But there has been no appropriate study about measles hepatitis in children. So we studied to facilitate the understanding of measles hepatitis in children by analyzing their clinical features and laboratory findings. METHODS: We investigated 164 patients who were admitted to the Department of Pediatrics, Seoul Red Cross Hospital due to measles from January 1985 to December 1994. We excluded other factors such as hepatitis A, B, C virus, Epstein-Barr virus and cytomegalovirus which could also result in hepatitis. According to the presence and the absence of coexistent complications 22 measles hepatitis patients were divided into two groups in which clinical characteristics and laboratory findings were analyzed as well. RESULTS: 1)Of total 164 patients there were 26 patients(15.9%) with measles hepatitis. 2)The ratio of male to female was 1.8:1. The range of age distribution was 6 months to thirteen years, and patients from 6 months to 3 years of age occupied 50% of the total patients. 3)The mean values of serum AST and ALT levels were 151+/-17.6 IU/L and 185+/-36.6 IU/L, espectively. And they were detected at 4.4+/-2.4 days after the first symptoms of measles were developed. 4)Major clinical characteristics were diarrhea in 22.7%, vomiting in 13.6%, hepatomegaly in 9.1%, and splenomegaly in 4.5%. Jaundice was not detected. 5)There was no difference between two groups in terms of serum albumin, globulin, bilirubin, AST and ALT(p>0.05). 6)Elevated serum AST and ALT levels returned to normal values within 2 weeks in 87.5%. CONCLUSIONS: Measles hepatitis develops with favorable outcome. Liver function test may well be done in cases of measles infection because hepatic dysfunction does not uncommonly occur in measles infection. Further study for the difference of the incidence of measles hepatitis according to the measles epidemics should be performed.
Age Distribution
;
Bilirubin
;
Child*
;
Cytomegalovirus
;
Diarrhea
;
Female
;
Hepatitis A
;
Hepatitis*
;
Hepatomegaly
;
Herpesvirus 4, Human
;
Humans
;
Incidence
;
Jaundice
;
Liver Function Tests
;
Male
;
Measles*
;
Pediatrics
;
Red Cross
;
Reference Values
;
Seoul
;
Serum Albumin
;
Splenomegaly
;
Vomiting
9.Induction of Metallothionein and Manganese-Containing Superoxide Dismutase by Paraquat in Peripheral White Blood Cells of Patients with Gastric Cancer.
Sung Pyo MOON ; Cheol Hee CHOI ; Yoon Jung CHA ; Jung Hwan CHANG ; Cheong Yong KIM ; Young Don MIN
Journal of the Korean Surgical Society 2000;59(6):751-758
PURPOSE: It is known that deficiency of metallothionein (MT) and manganese-containing superoxide dismutase (Mn-SOD), scavengers of reactive oxygen species, results in aging and carcinogenesis by inducing DNA damage. Paraquat can produce reactive oxygen species and induce antioxidants in human. In this study, an attempt was made to verify the relation between gastric carcinogensis and the induction rates of these antioxidants. METHODS: Peripheral blood of 24 randomly selected patients with gastric cancer, who were treated at Chosun University Hospital between Febuuary 1999 and December 1999, was examined. 3 male and 3 female patients in each decade from 40 to 70 years were selected. Twenty-four (24) volunteers with no laboratory, chemical, radiologic and endoscopic abnormalities during the same period were used for the normal control group. White blood cells were isolated from peripheral blood and incubated in culture media, including paraquat, for 18 hours at 37oC. MT and Mn-SOD mRNA expressions were examined using the reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: The induction rates of MT and Mn-SOD mRNA in the cancer group due to paraquat were lower than those in the control group. Also, the rates decreased in both groups with age. CONCLUSION: The inducibilities of MT and Mn-SOD mRNA by paraquat may play a role in the carcinogenesis of gastric cancer and in the aging process. Based on this result, patients with a high risk of gastric cancer should be screened actively for early detection.
Aging
;
Antioxidants
;
Carcinogenesis
;
Culture Media
;
DNA Damage
;
Female
;
Humans
;
Leukocytes*
;
Male
;
Metallothionein*
;
Paraquat*
;
Reactive Oxygen Species
;
RNA, Messenger
;
Stomach Neoplasms*
;
Superoxide Dismutase*
;
Superoxides*
;
Volunteers
10.MRI Findings of Extramedullary Hematopoiesis of the Spleen in Patient with Idiopathic Myelofibrosis: 2 case report.
Hyoung Seuk KIM ; Cheol Min PARK ; In Ho CHA ; Aeree KIM ; Moon Gyu LEE ; Yong Ho AUH
Journal of the Korean Radiological Society 1998;38(3):507-510
MRI findings of extramedullary hematopoiesis of the spleen have not been described in the literature. Wereport the MRI features of this condition, as seen in two patients and confirmed by fine needle biopsy. Threesmall masses(< or =3cm) were isointense on T1WI, hyperintense on T2WI, and enhanced after the injection of gadolinium.Two 6cm-sized masses were hypointense on both T1WI and T2WI, and showed no contrast enhancement.
Biopsy, Fine-Needle
;
Hematopoiesis, Extramedullary*
;
Humans
;
Magnetic Resonance Imaging*
;
Primary Myelofibrosis*
;
Spleen*